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EPITOPE TAGGING
Abstract
▪ Abstract Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. This review discusses the major advantages and limitations of epitope tagging and describes a number of recent applications. Major areas of application include monitoring protein expression, localizing proteins at the cellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Recently the method has also found use in transgenic and gene therapy studies and in the emerging fields of functional genomics and proteomics.
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Cited by
[16] Purification of proteins using polyhistidine affinity tags
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Diatoms, Biomineralization Processes, and Genomics
Hildebrand, M. (2008) · Chemical Reviews · doi:10.1021/cr078253z · Cited by 400
Affinity-purification coupled to mass spectrometry: basic principles and strategies
Dunham, W. H., Mullin, M., & Gingras, A. (2012) · PROTEOMICS · doi:10.1002/pmic.201100523 · Cited by 368
A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila
Bischof, J., Björklund, M., Furger, E., Schertel, C., Taipale, J., & Basler, K. (2013) · Development · doi:10.1242/dev.088757 · Cited by 323
Li, L., Fierer, J. O., Rapoport, T. A., & Howarth, M. (2014) · Journal of Molecular Biology · doi:10.1016/j.jmb.2013.10.021 · Cited by 317
Metadata
- Authors
- Jarvik, J. W., & Telmer, C. A.
- Venue
- Annual Review of Genetics
- Publisher
- Annual Reviews
- Published
- 1998-12
- Volume
- 32
- Issue
- 1
- Pages
- 601-618
- ISSN
- 0066-4197 (ISSN-L)
- 1545-2948
- OpenAlex
- W4240402038
- Cited by
- 163
- References
- 0
- FWCI
- 0.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: EPITOPE TAGGING. https://app.cochat.ai/papers/10.1146/annurev.genet.32.1.601
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- Jarvik, J. W., & Telmer, C. A. (1998). EPITOPE TAGGING. Annual Review of Genetics. https://doi.org/10.1146/annurev.genet.32.1.601
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