The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Genetic Engineering Using Homologous Recombination
Abstract
▪ Abstract In the past few years, in vivo technologies have emerged that, due to their efficiency and simplicity, may one day replace standard genetic engineering techniques. Constructs can be made on plasmids or directly on the Escherichia coli chromosome from PCR products or synthetic oligonucleotides by homologous recombination. This is possible because bacteriophage-encoded recombination functions efficiently recombine sequences with homologies as short as 35 to 50 base pairs. This technology, termed recombineering, is providing new ways to modify genes and segments of the chromosome. This review describes not only recombineering and its applications, but also summarizes homologous recombination in E. coli and early uses of homologous recombination to modify the bacterial chromosome. Finally, based on the premise that phage-mediated recombination functions act at replication forks, specific molecular models are proposed.
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Cites
Yang, X. W., Model, P., & Heintz, N. (1997) · Nature Biotechnology · doi:10.1038/nbt0997-859 · Cited by 544
Cited by
Nebert, D. W., & Dalton, T. P. (2006) · Nature Reviews Cancer · doi:10.1038/nrc2015 · Cited by 958
Phage therapy: From biological mechanisms to future directions
Strathdee, S. A., Hatfull, G. F., Mutalik, V. K., & Schooley, R. T. (2023) · Cell · doi:10.1016/j.cell.2022.11.017 · Cited by 817
Recombineering: a homologous recombination-based method of genetic engineering
Sharan, S. K., Thomason, L. C., Kuznetsov, S. G., & Court, D. L. (2009) · Nature Protocols · doi:10.1038/nprot.2008.227 · Cited by 785
Self-citationFu, J., Bian, X., Hu, S., Wang, H., Huang, F., Seibert, P. M., Plaza, A., Xia, L., Müller, R., Stewart, A. F., et al. (2012) · Nature Biotechnology · doi:10.1038/nbt.2183 · Cited by 448
Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N., & Court, D. L. (2014) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb0116s106 · Cited by 372
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Metadata
- Authors
- Court, D. L., Sawitzke, J. A., & Thomason, L. C.
- Venue
- Annual Review of Genetics
- Publisher
- Annual Reviews
- Published
- 2002-12
- Volume
- 36
- Issue
- 1
- Pages
- 361-388
- ISSN
- 0066-4197 (ISSN-L)
- 1545-2948
- OpenAlex
- W2155690692
- Cited by
- 505
- References
- 1
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- closed
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- Court, D. L., Sawitzke, J. A., & Thomason, L. C. (2002). Genetic Engineering Using Homologous Recombination. Annual Review of Genetics. https://doi.org/10.1146/annurev.genet.36.061102.093104
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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