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VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration

van Wetering, Sandra, van den Berk, Nadia, van Buul, Jaap D., Mul, Frederik P. J., Lommerse, Ingrid, Mous, Rogier, Klooster, Jean-Paul ten, Zwaginga, Jaap-Jan, Hordijk, Peter L.·2003·American Journal of Physiology-Cell Physiology
DOI 10.1152/ajpcell.00048.2003
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Abstract

Leukocyte adhesion is mediated totally and transendothelial migration partially by heterotypic interactions between the β 1- and β 2-integrins on the leukocytes and their ligands, Ig-like cell adhesion molecules (Ig-CAM), VCAM-1, and ICAM-1, on the endothelium. Both integrins and Ig-CAMs are known to have signaling capacities. In this study we analyzed the role of VCAM-1-mediated signaling in the control of endothelial cell-cell adhesion and leukocyte transendothelial migration. Antibody-mediated cross-linking of VCAM-1 on IL-1β-activated primary human umbilical vein endothelial cells (pHUVEC) induced actin stress fiber formation, contractility, and intercellular gaps. The effects induced by VCAM-1 cross-linking were inhibited by C3 toxin, indicating that the small GTPase p21Rho is involved. In addition, the effects of VCAM-1 were accompanied by activation of Rac, which we recently showed induce intercellular gaps in pHUVEC in a Rho-dependent fashion. With the use of a cell-permeable peptide inhibitor, it was shown that Rac signaling is required for VCAM-1-mediated loss of cell-cell adhesion. Furthermore, VCAM-1-mediated signaling toward cell-cell junctions was accompanied by, and dependent on, Rac-mediated production of reactive oxygen species and activation of p38 MAPK. In addition, it was found that inhibition of Rac-mediated signaling blocks transendothelial migration of monocytic U937 cells. Together, these data indicate that VCAM-1-induced, Rac-dependent signaling plays a key role in the modulation of vascular-endothelial cadherin-mediated endothelial cell-cell adhesion and leukocyte extravasation.

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Cited by

Metadata

Authors
van Wetering, S., van den Berk, N., van Buul, J. D., Mul, F. P. J., Lommerse, I., Mous, R., Klooster, J.-P. T., Zwaginga, J.-J., & Hordijk, P. L.
Venue
American Journal of Physiology-Cell Physiology
Publisher
American Physiological Society
Published
2003-08
Volume
285
Issue
2
Pages
C343-C352
ISSN
  • 0363-6143
  • 1522-1563
DOI
10.1152/ajpcell.00048.2003
Cited by
185
References
0
Language
en

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