The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Hydrogen peroxide stimulates ubiquitin-conjugating activity and expression of genes for specific E2 and E3 proteins in skeletal muscle myotubes
Abstract
Reactive oxygen species (ROS) are thought to promote muscle atrophy in chronic wasting diseases, but the underlying mechanism has not been determined. Here we show that H 2 O 2 stimulates ubiquitin conjugation to muscle proteins through transcriptional regulation of the enzymes (E2 and E3 proteins) that conjugate ubiquitin to muscle proteins. Incubation of C 2 C 12 myotubes with 100 μM H 2 O 2 increased the rate of 125 I-labeled ubiquitin conjugation to muscle proteins in whole cell extracts. This response required at least 4-h exposure to H 2 O 2 and persisted for at least 24 h. Preincubating myotubes with cycloheximide or actinomycin D blocked H 2 O 2 stimulation of ubiquitin-conjugating activity, suggesting that gene transcription is required. Northern blot analyses revealed that H 2 O 2 upregulates expression of specific E3 and E2 proteins that are thought to regulate muscle catabolism, including atrogin1/MAFbx, MuRF1, and E2 14k. These results suggest that ROS stimulate protein catabolism in skeletal muscle by upregulating the ubiquitin conjugation system.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
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Identification of ubiquitin ligases required for skeletal muscle atrophy.
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Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy
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Mechanisms of Muscle Wasting — The Role of the Ubiquitin–Proteasome Pathway
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Self-citationReactive oxygen in skeletal muscle. I. Intracellular oxidant kinetics and fatigue in vitro
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Self-citation
Cited by
IKKβ/NF-κB Activation Causes Severe Muscle Wasting in Mice
Cai, D., Frantz, J. D., Tawa, N. E., Melendez, P. A., Oh, B.-C., Lidov, H. G. W., Hasselgren, P.-O., Frontera, W. R., Lee, J., Glass, D. J., et al. (2004) · Cell · doi:10.1016/j.cell.2004.09.027 · Cited by 1,367
Skeletal muscle hypertrophy and atrophy signaling pathways
Glass, D. J. (2005) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2005.04.018 · Cited by 1,106
The Molecular Basis of Skeletal Muscle Atrophy
Jackman, R. W., & Kandarian, S. C. (2004) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00579.2003 · Cited by 1,008
Myostatin reduces Akt/TORC1/p70S6K signaling, inhibiting myoblast differentiation and myotube size
Trendelenburg, A. U., Meyer, A., Rohner, D., Boyle, J., Hatakeyama, S., & Glass, D. J. (2009) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00105.2009 · Cited by 792
The E3 Ligase MuRF1 Degrades Myosin Heavy Chain Protein in Dexamethasone-Treated Skeletal Muscle
Clarke, B. A., Drujan, D., Willis, M. S., Murphy, L. O., Corpina, R. A., Burova, E., Rakhilin, S. V., Stitt, T. N., Patterson, C., Latres, E., et al. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.09.009 · Cited by 683
Metadata
- Authors
- Li, Y.-P., Chen, Y., Li, A. S., & Reid, M. B.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2003-10
- Volume
- 285
- Issue
- 4
- Pages
- C806-C812
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2058996281
- Cited by
- 313
- References
- 28
- FWCI
- 3.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- Li, Y.-P., Chen, Y., Li, A. S., & Reid, M. B. (2003). Hydrogen peroxide stimulates ubiquitin-conjugating activity and expression of genes for specific E2 and E3 proteins in skeletal muscle myotubes. American Journal of Physiology-Cell Physiology. https://doi.org/10.1152/ajpcell.00129.2003
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