The DOI matches its Crossref record; 69 of the 69 references it cites were found in a registry or an index.
Regulation of placental amino acid transporter activity by mammalian target of rapamycin
Abstract
The activity of placental amino acid transporters is decreased in intrauterine growth restriction (IUGR), but the underlying regulatory mechanisms have not been established. Inhibition of the mammalian target of rapamycin (mTOR) signaling pathway has been shown to decrease the activity of the system L amino acid transporter in human placental villous fragments, and placental mTOR activity is decreased in IUGR. In the present study, we used cultured primary trophoblast cells to study mTOR regulation of placental amino acid transporters in more detail and to test the hypothesis that mTOR alters amino acid transport activity by changes in transporter expression. Inhibition of mTOR by rapamycin significantly reduced the activity of system A (−17%), system L (−28%), and taurine (−40%) amino acid transporters. mRNA expression of isoforms of the three amino acid transporter systems in response to mTOR inhibition was measured using quantitative real-time PCR. mRNA expression of l-type amino acid transporter 1 (LAT1; a system L isoform) and taurine transporter was reduced by 13% and 50%, respectively; however, mTOR inhibition did not alter the mRNA expression of system A isoforms (sodium-coupled neutral amino acid transporter-1, -2, and -4), LAT2, or 4F2hc. Rapamycin treatment did not significantly affect the protein expression of any of the transporter isoforms. We conclude that mTOR signaling regulates the activity of key placental amino acid transporters and that this effect is not due to a decrease in total protein expression. These data suggest that mTOR regulates placental amino acid transporters by posttranslational modifications or by affecting transporter translocation to the plasma membrane.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In addition, the mTOR pathway regulates the activity of system A, system L and taurine amino acid transporters in the placenta at the post-translational level, either through modifications or by influencing translocation to the apical membrane (469).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [469]
Citations
Cites
TOR signaling in growth and metabolism
Wullschleger, S., Loewith, R., & Hall, M. N. (2006) · Cell · doi:10.1016/j.cell.2006.01.016 · Cited by 5,787
Prolonged Rapamycin Treatment Inhibits mTORC2 Assembly and Akt/PKB
Sarbassov, D. D., Ali, S. M., Sengupta, S., Sheen, J.-H., Hsu, P. P., Bagley, A. F., Markhard, A. L., & Sabatini, D. M. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.03.029 · Cited by 2,365
Boney, C. M., Verma, A., Tucker, R., & Vohr, B. R. (2005) · Pediatrics · doi:10.1542/peds.2004-1808 · Cited by 2,303
Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive
Jacinto, E., Loewith, R., Schmidt, A., Lin, S., Rüegg, M. A., Hall, A., & Hall, M. N. (2004) · Nature Cell Biology · doi:10.1038/ncb1183 · Cited by 2,097
Kliman, H. J., Nestler, J. E., SERMASI, E., Sanger, J. M., & Strauss, J. F. (1986) · Endocrinology · doi:10.1210/endo-118-4-1567 · Cited by 1,572
Birth Weight and Adult Hypertension, Diabetes Mellitus, and Obesity in US Men
Curhan, G. C., Willett, W. C., Rimm, E. B., Spiegelman, D. L., Ascherio, A., & Stampfer, M. J. (1996) · Circulation · doi:10.1161/01.cir.94.12.3246 · Cited by 917
Nobukuni, T., Joaquin, M., Roccio, M., Dann, S. G., Kim, S. Y., Gulati, P., Byfield, M. P., Backer, J., Natt, F., Bos, J. L., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506925102 · Cited by 775
Akt Maintains Cell Size and Survival by Increasing mTOR-dependent Nutrient Uptake
Edinger, A. L., & Thompson, C. B. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.01-12-0584 · Cited by 589
hVps34 Is a Nutrient-regulated Lipid Kinase Required for Activation of p70 S6 Kinase
Byfield, M. P., Murray, J. T., & Backer, J. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m507201200 · Cited by 539
Pregnancy Outcomes in Women With Gestational Diabetes Compared With the General Obstetric Population
Casey, B. M. (1997) · Obstetrics and Gynecology · doi:10.1016/s0029-7844(97)00542-5 · Cited by 434
Flagged first, then by citations
Cited by
Sorted by citations
Items 101–150 of 150
Labor inhibits placental mechanistic target of rapamycin complex 1 signaling
Lager, S., Aye, I. L. M. H., Gaccioli, F., Ramirez, V. I., Jansson, T., & Powell, T. L. (2014) · Placenta · doi:10.1016/j.placenta.2014.10.006 · Cited by 15
Self-citationViral model of maternal immune activation alters placental AMPK and mTORC1 signaling in rats
McColl, E. R., & Piquette‐Miller, M. (2021) · Placenta · doi:10.1016/j.placenta.2021.07.002 · Cited by 14
Faehling, M., Wienhausen-Wilke, V., Fallscheer, S., Trinajstic-Schulz, B., Weber, J., & Leschke, M. (2015) · Sarcoidosis vasculitis and diffuse lung diseases · Cited by 14
McIntyre, K. R., Hayward, C. E., Sibley, C. P., Greenwood, S. L., & Dilworth, M. R. (2019) · The Journal of Physiology · doi:10.1113/jp278226 · Cited by 14
LAT1-like transporters regulate dopaminergic transmission and sleep in Drosophila
Aboudhiaf, S., Alves, G., Parrot, S., Amri, M., Simonnet, M. M., Grosjean, Y., Manière, G., & Seugnet, L. (2018) · SLEEP · doi:10.1093/sleep/zsy137 · Cited by 16
Tanaka, K., Tanaka, H., Tachibana, R., Yoshikawa, K., Kawamura, T., Takakura, S., Takeuchi, H., & Ikeda, T. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23031474 · Cited by 13
Götzberger, M., Kaiser, C. A., Landauer, N., Dieterle, C., Heldwein, W., & Schiemann, U. (2008) · PubMed · Cited by 13
Li, Q., Zhang, Q., Su, S., Yang, S., Shao, J., Guan, W., & Zhang, S. (2024) · Journal of Advanced Research · doi:10.1016/j.jare.2024.12.029 · Cited by 11
Framarino‐dei‐Malatesta, M., Derme, M., Napoli, A., Iaria, G., Manzia, T. M., Orlando, G., Casorelli, A., & Berloco, P. B. (2014) · Transplantation Proceedings · doi:10.1016/j.transproceed.2014.07.047 · Cited by 11
Fahlbusch, F. B., Hartner, A., Menendez‐Castro, C., Nögel, S. C., Marek, I., Beckmann, M. W., Schleußner, E., Ruebner, M., Huebner, H., Dörr, H. G., et al. (2015) · Journal of Developmental Origins of Health and Disease · doi:10.1017/s2040174415001154 · Cited by 11
Glucocorticoid regulation of amino acid transport in primary human trophoblast cells
Vaughan, O. R., Powell, T. L., & Jansson, T. (2019) · Journal of Molecular Endocrinology · doi:10.1530/jme-19-0183 · Cited by 11
Self-citationJessel, R. H., Rosario, F. J., Chen, Y., Erickson, K., Teal, S. B., Kramer, A. W., Cotton, E., Ryan, S., Jansson, T., & Powell, T. L. (2019) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2019.108305 · Cited by 11
Self-citationVitamin D stimulates placental L-type amino acid transporter 1 (LAT1) in preeclampsia
Jia, X., Cao, Y., Ye, L., Liu, X., Huang, Y., Yuan, X., Lu, C., Xu, J., & Zhu, H. F. (2022) · Scientific Reports · doi:10.1038/s41598-022-08641-y · Cited by 11
Rosario, F. J., Chopra, A., Biggar, K. K., Powell, T. L., Gupta, M. B., & Jansson, T. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24087273 · Cited by 11
Self-citationInduction of amino acid transporters expression by endurance exercise in rat skeletal muscle
Murakami, T., & Yoshinaga, M. (2013) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2013.08.094 · Cited by 10
Placental Function in Maternofetal Exchange
Sibley, C. P., & Dilworth, M. R. (2020) · Elsevier eBooks · doi:10.1016/b978-0-7020-6956-7.00008-7 · Cited by 10
Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant Mice
Wang, X., Tan, D., Ma, J., Liang, H., Zhang, Q., Tan, Y., Wang, J., & Luo, W. (2016) · Nutrients · doi:10.3390/nu8110704 · Cited by 10
McCoard, S. A., Haack, N. A., Heiser, A., & Maclean, P. H. (2023) · Theriogenology · doi:10.1016/j.theriogenology.2023.03.009 · Cited by 9
Tain, Y., & Hsu, C. (2023) · Metabolites · doi:10.3390/metabo13030418 · Cited by 7
Roeca, C. M., Silva, E., Barentsen, C., Powell, T. L., & Jansson, T. (2020) · Molecular Human Reproduction · doi:10.1093/molehr/gaaa047 · Cited by 7
Self-citationThe Placenta and Developmental Origins of Health and Disease
Cleal, J. K., & Lewis, R. M. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-801383-0.00022-0 · Cited by 7
Viola, I., Accornero, P., Manenti, I., Miretti, S., Baratta, M., & Toschi, P. (2024) · Placenta · doi:10.1016/j.placenta.2024.09.011 · Cited by 7
Syncytin-1 deficiency impairs placental nutrient transport via PI3K/Akt/mTOR signaling
Chen, X., Gong, X.-X., Xiong, Z.-L., Zhou, D., Yang, J., Zhang, H., Xue, Y.-S., Wang, Y.-N., & Chen, H. (2025) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2025.110060 · Cited by 6
Metabolic consequences of altered kidney glucose reabsorption under normoglycemic conditions
Ahmad, M., Permyakova, A., Baraghithy, S., Sahu, N., Abramovich, I., Agranovich, B., Shalev, O., Kogot‐Levin, A., Nemirovski, A., Gottlieb, E., et al. (2025) · Molecular Metabolism · doi:10.1016/j.molmet.2025.102192 · Cited by 6
RISING STARS: Mechanistic insights into maternal–fetal cross talk and islet beta-cell development
Jo, S., & Alejandro, E. U. (2023) · Journal of Endocrinology · doi:10.1530/joe-23-0069 · Cited by 7
Orzabal, M. R., Naik, V. D., Lee, J., Wu, G., & Ramadoss, J. (2021) · Metabolism Open · doi:10.1016/j.metop.2021.100107 · Cited by 4
Placental Function in Intrauterine Growth Restriction
Chen, Y., & Jansson, T. (2016) · Elsevier eBooks · doi:10.1016/b978-0-323-35214-7.00017-2 · Cited by 4
Self-citationShao, X., Wu, P., Feng, L., Jiang, W., Liu, Y., Kuang, S., Tang, L. Y., & Zhou, X. (2020) · Aquaculture Nutrition · doi:10.1111/anu.13178 · Cited by 4
Luo, W., Zhang, H., Zhang, Y., Liang, P., Wang, X., Ma, J., Tan, D., Tan, Y., Song, J., Ji, P., et al. (2020) · PubMed Central · Cited by 4
Rosario, F. J., Urschitz, J., Razavy, H., Elston, M., Powell, T. L., & Jansson, T. (2025) · Clinical Science · doi:10.1042/cs20243293 · Cited by 5
Self-citationTLR5 Supports Development of Placental Labyrinthine Zone in Mice
Yiu, J. H. C., Cheung, S. W. M., Cai, J., Chan, K.-S., Chen, J., Cheong, L. Y., Chau, H.-T., Xu, A., Li, R., & Woo, C. W. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.711253 · Cited by 4
Placental amino acid transporters
Jansson, T., & Powell, T. L. (2010) · Cambridge University Press eBooks · doi:10.1017/cbo9780511933806.013 · Cited by 3
Self-citationBasic Experimental and Clinical Advances in the Mechanisms Underlying Abnormal Pregnancy Outcomes
Regnault, T. R. H., Nijland, M. J., Budge, H., & Morrison, J. L. (2013) · Journal of Pregnancy · doi:10.1155/2013/327638 · Cited by 3
Conduit, S., Zhang, C. X. W., Pearce, W., Guillermet-Guibert, J., Sferruzzi-Perri, A., & Vanhaesebroeck, B. (2025) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-025-05937-w · Cited by 2
Wang, D., Wu, X., Lü, D., Li, Y., & Zhang, P. (2020) · Current Molecular Medicine · doi:10.2174/1566524020666200726221735 · Cited by 2
Everolimus and Giant-Cell Astrocytomas in Tuberous Sclerosis
Jia, J., Xiong, L., & Chen, S. (2011) · New England Journal of Medicine · doi:10.1056/nejmc1013587 · Cited by 2
Rosario, F. J., Urschitz, J., Razavy, H., Elston, M., Powell, T. L., & Jansson, T. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.02.20.581180 · Cited by 2
Self-citationBidne, K. L., Erickson, K., Powell, T. L., & Jansson, T. (2025) · Biology of Reproduction · doi:10.1093/biolre/ioaf007 · Cited by 1
Self-citationRole of Amino Acid Transporters in Protein Metabolism
Taylor, P. M. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802167-5.00005-0 · Cited by 1
Roos, S. (2008) · Gothenburg University Publications Electronic Archive (Gothenburg University)
Self-citationEvaluation of Placental Transport Function
Dilworth, M. R., Sibley, C. P., & Glazier, J. D. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-394445-0.00058-8
Role of LAT-1 in trophoblast migration and embryo implantation
Chrostowski, M. K. (2009) · doi:10.7301/z02j693j
Shimada, H., Sakuragi, T., Barentsen, K., Erickson, K., Urschitz, J., Powell, T. L., Jansson, T., & Rosario, F. J. (2025) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2025.11.025
Self-citationSedlmeier, E.-M., Meyer, D. M., Stecher, L., Sailer, M., Daniel, H., Hauner, H., & Bader, B. (2021) · Figshare · doi:10.6084/m9.figshare.14157643
Sedlmeier, E.-M., Meyer, D. M., Stecher, L., Sailer, M., Daniel, H., Hauner, H., & Bader, B. (2021) · Figshare · doi:10.6084/m9.figshare.14157646
Sedlmeier, E.-M., Meyer, D. M., Stecher, L., Sailer, M., Daniel, H., Hauner, H., & Bader, B. (2021) · Figshare · doi:10.6084/m9.figshare.14157655
Sedlmeier, E.-M., Meyer, D. M., Stecher, L., Sailer, M., Daniel, H., Hauner, H., & Bader, B. (2021) · Figshare · doi:10.6084/m9.figshare.14157652
Sedlmeier, E.-M., Meyer, D. M., Stecher, L., Sailer, M., Daniel, H., Hauner, H., & Bader, B. (2021) · Figshare · doi:10.6084/m9.figshare.14157649
Nogues, P. (2019) · theses.fr (ABES) · doi:10.70675/c3ecfee5ze428z4395za563za025758f71a2
Graafland, N., Posthumus, A. G., Mulders, A. G. M. G. J., Gaillard, R., Koning, A. H. J., Jaddoe, V. W. V., Steegers, E. A. P., & Rousian, M. (2026) · Human Reproduction · doi:10.1093/humrep/deag125
Metadata
- Authors
- Roos, S., Kanai, Y., Prasad, P. D., Powell, T. L., & Jansson, T.
- Affiliations
- University of Gothenburg (SE)
- The University of Osaka (JP)
- University of Cincinnati (US)
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2009-01
- Volume
- 296
- Issue
- 1
- Pages
- C142-C150
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2131629100
- Cited by
- 150
- References
- 69
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 6 · 2023: 5 · 2022: 6 · 2021: 12 · 2020: 12 · 2019: 10 · 2018: 8 · 2017: 13 · 2016: 10 · 2015: 11 · 2014: 12 · 2013: 8 · 2012: 16
- FWCI
- 5.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Keywords
- mTOR signaling, fetal growth restriction, system L amino acid transporter, system A amino acid transporter, taurine transporter, LAT1, rapamycin, trophoblast, post-translational regulation, placenta
- MeSH terms
- Taurine Transporters, Cell Survival, Cells, Cultured, Female, Humans, Membrane Glycoproteins, Pregnancy, Protein Kinases, Protein Processing, Post-Translational, RNA, Messenger, Time Factors, Trophoblasts, Signal Transduction, Apoptosis, Sirolimus, Protein Transport, Membrane Transport Proteins, Amino Acid Transport Systems, Amino Acid Transport System A, Amino Acid Transport System L, TOR Serine-Threonine Kinases
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
