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Effect of reduced oxygen tension on chondrogenesis and osteogenesis in adipose-derived mesenchymal cells
Abstract
Recent studies have demonstrated that adipose-derived mesenchymal cells (AMCs) offer great promise for cell-based therapies because of their ability to differentiate toward bone, cartilage, and fat. Given that cartilage is an avascular tissue and that mesenchymal cells experience hypoxia during prechondrogenic condensation in endochondral ossification, the goal of this study was to understand the influence of oxygen tension on AMC differentiation into bone and cartilage. In vitro chondrogenesis was induced using a three-dimensional micromass culture model supplemented with TGF-β1. Collagen II production and extracellular matrix proteoglycans were assessed with immunohistochemistry and Alcian blue staining, respectively. Strikingly, micromasses differentiated in reduced oxygen tension (2% O 2) showed markedly decreased chondrogenesis. Osteogenesis was induced using osteogenic medium supplemented with retinoic acid or vitamin D and was assessed with alkaline phosphatase activity and mineralization. AMCs differentiated in both 21 and 2% O 2 environments. However, osteogenesis was severely diminished in a low-oxygen environment. These data demonstrated that hypoxia strongly inhibits in vitro chondrogenesis and osteogenesis in AMCs.
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Cited by
Oxygen in Stem Cell Biology: A Critical Component of the Stem Cell Niche
Mohyeldin, A., Garzón-Muvdi, T., & Quiñones-Hinojosa, A. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.07.007 · Cited by 1,535
Hypoxia enhances proliferation and tissue formation of human mesenchymal stem cells
Grayson, W. L., Zhao, F., Bunnell, B., & Ma, T. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.05.054 · Cited by 521
Demoor, M., Ollitrault, D., Gomez-Leduc, T., Bouyoucef, M., Hervieu, M., Fabre, H., Lafont, J., Denoix, J.-M., Audigié, F., Mallein-Gerin, F., et al. (2014) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2014.02.030 · Cited by 296
The Effect of Hypoxia on Mesenchymal Stem Cell Biology
Ejtehadifar, M., Shamsasenjan, K., Movassaghpour, A., Akbarzadehlaleh, P., Dehdilani, N., Abbasi, P., Molaeipour, Z., & Saleh, M. (2015) · Advanced Pharmaceutical Bulletin · doi:10.15171/apb.2015.021 · Cited by 235
Berniakovich, I., & Giorgio, M. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms14012119 · Cited by 103
Metadata
- Authors
- Malladi, P., Xu, Y., Chiou, M., Giaccia, A. J., & Longaker, M. T.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2006-04
- Volume
- 290
- Issue
- 4
- Pages
- C1139-C1146
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2043814008
- Cited by
- 301
- References
- 0
- FWCI
- 4.87
- Type
- journal article
- Language
- en
- Open access
- closed
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- Malladi, P., Xu, Y., Chiou, M., Giaccia, A. J., & Longaker, M. T. (2006). Effect of reduced oxygen tension on chondrogenesis and osteogenesis in adipose-derived mesenchymal cells. American Journal of Physiology-Cell Physiology. https://doi.org/10.1152/ajpcell.00415.2005
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