Ca(2+)-insensitive sustained contraction of skinned smooth muscle after acidic ADP treatment
Abstract
After an acidic treatment in the presence of ADP, Triton X-100-skinned rabbit aortic smooth muscle strips were found to develop a large sustained, Ca(2+)-insensitive tension when returned to a relaxing solution with neutral pH. The presence of ADP during treatment was essential for the manifestation of the Ca(2+)-insensitive contraction. This contraction was reversibly eliminated by withdrawal of MgATP or addition of vanadate and was found to be accompanied by an extraordinarily high level of 20-kDa myosin light-chain (MLC20) phosphorylation. The rate constant for dephosphorylation of MLC20 in treated strips was about one-twenty-fifth that in untreated control, when determined after removal of Ca2+, Mg2+, and ATP. Two-dimensional phosphopeptide mapping of tryptic digests of MLC20 showed that most incorporated phosphate was in the peptides which would be phosphorylated by myosin light-chain kinase. These results provide strong evidence that ADP inactivates myosin light-chain phosphatase under acidic conditions.
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In apparently healthy subjects, production of hyperglycaemia by continuous glucose infusion for up to 42 h is associated with enhancements of insulin sensitivity and beta-cell function rather than insulin resistance and beta-cell dysfunction.
“This thesis is based on the observation that in apparently healthy subjects the production of hyperglycaemia by a continuous infusion of glucose for periods of up to 42-h is associated with enhancements of insulin sensitivity [141] and beta-cell function [141, 142, 143, 144], rather than the production of insulin resistance and beta-cell dysfunction.”
From The relative contributions of insulin resistance and beta-cell dysfunction to the pathophysiology of type 2 diabetes (2003) · cites this paper as [143]
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- Authors
- Morimoto, S., & Ogawa, Y.
- Affiliations
- Juntendo University (JP)
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 1995-01-01
- Volume
- 268
- Issue
- 1
- Pages
- C21-C29
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2700419483
- Cited by
- 18
- References
- 17
- Citations by year
- 2022: 1
- FWCI
- 0.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- sustained contraction, aortic smooth muscle, myosin light chain phosphorylation, myosin light chain kinase, vanadate, MgATP
- MeSH terms
- Acids, Adenosine Diphosphate, Adenosine Triphosphate, Animals, Calcium, Drug Resistance, Histological Techniques, Hydrogen-Ion Concentration, Isometric Contraction, Muscle, Smooth, Vascular, Myosins, Myosin-Light-Chain Kinase, Phosphorylation, Rabbits, Vasoconstriction
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