The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies
Abstract
The Na-K-Cl cotransporter (NKCC) is present in most animal cells where it functions in cell volume homeostasis and epithelial salt transport. We developed six monoclonal antibodies (designated T4, T8, T9, T10, T12, and T14) against a fusion protein fragment encompassing the carboxy-terminal 310 amino acids of the human colonic NKCC. These T antibodies selectively recognized putative NKCC proteins in a diverse variety of animal tissues. Western blot analysis of membranes isolated from 23 types of cells identified single bands of immunoreactive protein ranging in mass from 146 to 205 kDa. The amount of immunoreactive protein detected in these cells correlated with loop diuretic binding site density. Proteins identified previously as Na-K-Cl cotransporters by loop diuretic photoaffinity labeling were mutually recognized by multiple T antibodies. Most of the T antibodies effectively immunoprecipitated the denatured form of the NKCC protein. Immunocytochemical studies on the rabbit parotid gland demonstrated that NKCC is restricted to the basolateral margin of the acinar cells and absent from the ducts, in accord with the central role of Na-K-Cl cotransport in chloride secretion. In the rabbit kidney, NKCC was localized to the apical membrane of thick ascending limb cells, consistent with its role in chloride reabsorption.
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Molecular cloning and functional expression of the bumetanide-sensitive Na-K-Cl cotransporter.
Xu, J. C., Lytle, C., Zhu, T. T., Payne, J. A., Benz, E., & Forbush, B. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.6.2201 · Cited by 424
Self-citationPayne, J. A., & Forbush, B. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.10.4544 · Cited by 286
Self-citationPayne, J. A., Xu, J.-C., Haas, M., Lytle, C. Y., Ward, D., & Forbush, B. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.30.17977 · Cited by 257
Self-citationJennings, M. L., & al-Rohil, N. (1990) · The Journal of general physiology · doi:10.1085/jgp.95.6.1021 · Cited by 200
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Na+/K+/2Cl− cotransport in medullary thick ascending limb cells: kinetics and bumetanide binding
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Cited by
Guerrero‐Hue, M., García‐Caballero, C., Palomino‐Antolín, A., Rubio‐Navarro, A., Vázquez‐Carballo, C., Herencia, C., Martín‐Sanchez, D., Farré‐Alins, V., Egea, J., Cannata, P., et al. (2019) · The FASEB Journal · doi:10.1096/fj.201900077r · Cited by 268
Su, G., Kintner, D. B., Flagella, M., Shull, G. E., & Sun, D. (2002) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00538.2001 · Cited by 169
O'Donnell, M. E., Lam, T. I., Tran, L. Q., Foroutan, S., & Anderson, S. E. (2006) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600278 · Cited by 107
Foroutan, S., Brillault, J., Forbush, B., & O’Donnell, M. E. (2005) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00257.2005 · Cited by 81
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Metadata
- Authors
- Lytle, C., Xu, J. C., Biemesderfer, D., & Forbush, B.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 1995-12-01
- Volume
- 269
- Issue
- 6
- Pages
- C1496-C1505
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2287827025
- Cited by
- 367
- References
- 9
- FWCI
- 2.61
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lytle, C., Xu, J. C., Biemesderfer, D., & Forbush, B. (1995). Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies. American Journal of Physiology-Cell Physiology. https://doi.org/10.1152/ajpcell.1995.269.6.c1496
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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