The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Expression of the Na(+)-K(+)-2Cl- cotransporter BSC2 in the nervous system
Abstract
We used in situ hybridization and immunocytochemistry with polyclonal antibodies against the mouse bumetanide-sensitive Na(+)-K(+)-2Cl- cotransporter (mBSC2) to determine the location of this cotransporter in rat brain. Northern blots and in situ hybridization showed the presence of cotransporter mRNA in the brain, with an especially high level of expression in the choroid plexus (CP). Affinity-purified anti-BSC2 antibody identified proteins of 145-155 kDa on Western blot analysis and immunoprecipitation of brain and CP membrane protein. Indirect immunofluorescence demonstrated that BSC2 protein is located on the apical surface of the CP and is heterogeneously distributed in cell bodies and dendrites of neurons in the central and peripheral nervous system. The apical localization of BSC2 in the CP was confirmed by 86Rb+ uptakes in primary cultures of CP cells grown on permeable filters and confocal immunofluorescence microscopy. The apical localization of the cotransporter in CP epithelium suggests a role for the cotransporter in cerebrospinal fluid K+ homeostasis. In neurons, the cotransporter may help regulate intracellular Cl- concentration and thereby affect neuronal response to gamma-aminobutyric acid.
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Molecular cloning and functional expression of the bumetanide-sensitive Na-K-Cl cotransporter.
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Thyroxine transport to the brain: role of protein synthesis by the choroid plexus
Southwell, B. R. (1993) · Endocrinology · doi:10.1210/en.133.5.2116 · Cited by 140
Transferrin gene expression and synthesis by cultured choroid plexus epithelial cells
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Na+,K+-ATPase in the Choroid Plexus
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Physiology of cell volume regulation in vertebrates
Hoffmann, E. K., Lambert, I. H., & Pedersen, S. F. (2009) · Physiological Reviews · doi:10.1152/physrev.00037.2007 · Cited by 1,441
Estradiol and the Developing Brain
McCARTHY, M. M. (2008) · Physiological Reviews · doi:10.1152/physrev.00010.2007 · Cited by 885
Cation–chloride co-transporters in neuronal communication, development and trauma
Payne, J. A., Rivera, C., Voipio, J., & Kaila, K. (2003) · Trends in Neurosciences · doi:10.1016/s0166-2236(03)00068-7 · Cited by 832
The molecular basis of water transport in the brain
Amiry-Moghaddam, M., & Ottersen, O. P. (2003) · Nature Reviews Neuroscience · doi:10.1038/nrn1252 · Cited by 733
Cerebrospinal fluid secretion by the choroid plexus
Damkier, H. H., Brown, P. D., & Praetorius, J. (2013) · Physiological Reviews · doi:10.1152/physrev.00004.2013 · Cited by 606
Metadata
- Authors
- Plotkin, M. D., Kaplan, M. R., Peterson, L. N., Gullans, S. R., Hebert, S. C., & Delpire, E.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 1997-01-01
- Volume
- 272
- Issue
- 1
- Pages
- C173-C183
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2230118829
- Cited by
- 263
- References
- 12
- FWCI
- 3.38
- Type
- journal article
- Language
- en
- Open access
- closed
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- Plotkin, M. D., Kaplan, M. R., Peterson, L. N., Gullans, S. R., Hebert, S. C., & Delpire, E. (1997). Expression of the Na(+)-K(+)-2Cl- cotransporter BSC2 in the nervous system. American Journal of Physiology-Cell Physiology. https://doi.org/10.1152/ajpcell.1997.272.1.c173
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