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Skeletal muscle glucose uptake during contraction is regulated by nitric oxide and ROS independently of AMPK
Abstract
Reactive oxygen species (ROS) and nitric oxide (NO) have been implicated in the regulation of skeletal muscle glucose uptake during contraction, and there is evidence that they do so via interaction with AMP-activated protein kinase (AMPK). In this study, we tested the hypothesis that ROS and NO regulate skeletal muscle glucose uptake during contraction via an AMPK-independent mechanism. Isolated extensor digitorum longus (EDL) and soleus muscles from mice that expressed a muscle-specific kinase dead AMPKα2 isoform (AMPK-KD) and wild-type litter mates (WT) were stimulated to contract, and glucose uptake was measured in the presence or absence of the antioxidant N-acetyl-l-cysteine (NAC) or the nitric oxide synthase (NOS) inhibitor N G -monomethyl-l-arginine (l-NMMA). Contraction increased AMPKα2 activity in WT but not AMPK-KD EDL muscles. However, contraction increased glucose uptake in the EDL and soleus muscles of AMPK-KD and WT mice to a similar extent. In EDL muscles, NAC and l-NMMA prevented contraction-stimulated increases in oxidant levels (dichloroflourescein fluorescence) and NOS activity, respectively, and attenuated contraction-stimulated glucose uptake in both genotypes to a similar extent. In soleus muscles of AMPK-KD and WT mice, NAC prevented contraction-stimulated glucose uptake and l-NMMA had no effect. This is likely attributed to the relative lack of neuronal NOS in the soleus muscles compared with EDL muscles. Contraction increased AMPKα Thr 172 phosphorylation in EDL and soleus muscles of WT but not AMPK-KD mice, and this was not affected by NAC or l-NMMA treatment. In conclusion, ROS and NO are involved in regulating skeletal muscle glucose uptake during contraction via an AMPK-independent mechanism.
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“AMPK α2 KO and α2 KD and α2iTg mice have normal or only modest reductions in glucose uptake during contractions [4,26,31,32,118-120].”
From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [120]
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Molecular mechanisms of ROS production and oxidative stress in diabetes
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AMPK in skeletal muscle function and metabolism
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PGC-1α-mediated regulation of mitochondrial function and physiological implications
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Metadata
- Authors
- Merry, T. L., Steinberg, G. R., Lynch, G. S., & McConell, G. K.
- Affiliations
- The University of Melbourne (AU)
- McMaster University (CA)
- Exercise Physiology and Metabolism Laboratory,
- Corresponding author
- Merry, Troy L.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2010-03
- Volume
- 298
- Issue
- 3
- Pages
- E577-E585
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2103404817
- Cited by
- 122
- References
- 57
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 1 · 2023: 4 · 2022: 7 · 2021: 5 · 2020: 17 · 2019: 8 · 2018: 7 · 2017: 5 · 2016: 10 · 2015: 14 · 2014: 10 · 2013: 7 · 2012: 6
- FWCI
- 2.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Victoria University Research Repository (Victoria University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Exercise and Physiological Responses
- Keywords
- skeletal muscle glucose uptake, muscle contraction, nitric oxide, reactive oxygen species, AMPK, AMPK-independent mechanism, AMPKα2, nitric oxide synthase, neuronal nitric oxide synthase, oxidative stress, N-acetylcysteine, L-NMMA, extensor digitorum longus, soleus muscle
- MeSH terms
- AMP-Activated Protein Kinase Kinases, Animals, Female, Glucose, Male, Mice, Inbred C57BL, Mice, Transgenic, Muscle Contraction, Nitric Oxide, Protein Kinases, Signal Transduction, Reactive Oxygen Species, Muscle, Skeletal, Mice
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