The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
A pipeline for multidimensional confocal analysis of mitochondrial morphology, function, and dynamics in pancreatic β-cells
Open accessAbstract
Live-cell imaging of mitochondrial function and dynamics can provide vital insights into both physiology and pathophysiology, including of metabolic diseases like type 2 diabetes. However, without super-resolution microscopy and commercial analysis software, it is challenging to accurately extract features from dense multilayered mitochondrial networks, such as those in insulin-secreting pancreatic β-cells. Motivated by this, we developed a comprehensive pipeline and associated ImageJ plugin that enables 2D/3D quantification of mitochondrial network morphology and dynamics in mouse β-cells and by extension other similarly challenging cell types. The approach is based on standard confocal microscopy and shareware, making it widely accessible. The pipeline was validated using mitochondrial photolabeling and unsupervised cluster analysis and is capable of morphological and functional analyses on a per-organelle basis, including in 4D ( xyzt). Overall, this tool offers a powerful framework for multiplexed analysis of mitochondrial state/function and provides a valuable resource to accelerate mitochondrial research in health and disease.
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Hernansanz-Agustín, P., Morales-Vidal, C., Calvo, E., Natale, P., Martí-Mateos, Y., Jaroszewicz, S. N., Cabrera-Alarcón, J. L., Acín-Pérez, R., López-Montero, I., Vázquez, J., et al. (2024) · Cell · doi:10.1016/j.cell.2024.08.045 · Cited by 34
Metadata
- Authors
- Chaudhry, A., Shi, R., & Luciani, D. S.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2020-02-01
- Volume
- 318
- Issue
- 2
- Pages
- E87-E101
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2995604272
- Cited by
- 423
- References
- 51
- FWCI
- 3.80
- Type
- journal article
- Language
- en
- Open access
- green
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- Chaudhry, A., Shi, R., & Luciani, D. S. (2020). A pipeline for multidimensional confocal analysis of mitochondrial morphology, function, and dynamics in pancreatic β-cells. American Journal of Physiology-Endocrinology and Metabolism. https://doi.org/10.1152/ajpendo.00457.2019
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