Effect of insulin and glucocorticoids on glucose transporters in rat adipocytes
Abstract
The ability of glucocorticoids to modify the effect of insulin on glucose transport was investigated in both intact isolated rat adipocytes and in membranes isolated from hormone-treated adipocytes. In intact adipocytes, dexamethasone, a potent synthetic glucocorticoid, inhibited insulin-stimulated 3-O-methylglucose transport at all concentrations of insulin tested (0-2,000 microU/ml). Insulin sensitivity, as well as the maximal response to insulin, was decreased by dexamethasone in the absence of a change in insulin binding. The inhibition was observed regardless of which hormone acted first, was blocked by actinomycin D, and resulted from a decrease in Vmax rather than an increase in Kt of transport. In plasma membranes isolated from insulin-treated adipocytes, glucose transport activity and the amount of glucose transporter covalently labeled with [3H]cytochalasin B were increased in parallel in a dose-dependent fashion. The amount of labeled transporter in a low-density microsomal fraction (LDMF) was decreased in a reciprocal fashion. In contrast, addition of dexamethasone to insulin-stimulated cells caused decreases in both transport activity and amount of labeled transporter in the plasma membranes. This was accompanied by a small increase in the amount of [3H]cytochalasin B incorporated into the glucose transporter in the LDMF. These results are consistent with both insulin and glucocorticoids altering the distribution of glucose transporters between the plasma membrane and LDMF, in opposite directions.
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Cortisol regulates glucose uptake by antagonising insulin-mediated translocation of GLUTs in fat and muscle.
“CORT regulates glucose uptake by antagonising the insulin-mediated translocation of GLUTs in fat and muscle ( 32-35 ),”
From Dynamic modulation of glucose utilisation by glucocorticoid rhythms in health and disease (2020) · cites this paper as [34]
Kinetic rates involved in GLUT translocation, including the effects of insulin and glucocorticoids, were estimated from prior studies.
“the kinetic rates involved in the translocation of GLUT transporters, including the effects of insulin and glucocorticoids, were estimated from ( 27, 34, 46 );”
From Dynamic modulation of glucose utilisation by glucocorticoid rhythms in health and disease (2020) · cites this paper as [34]
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Fisher, R. E. (2013) · The Atrium (University of Guelph)
- Authors
- Carter-Su, C., & Okamoto, K.
- Affiliations
- University of Michigan (US)
- ORCID
- Okamoto, K.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 1987-04-01
- Volume
- 252
- Issue
- 4
- Pages
- E441-E453
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2104991071
- Cited by
- 71
- References
- 2
- Citations by year
- 2024: 1 · 2023: 13 · 2022: 2 · 2020: 2 · 2019: 1 · 2018: 2 · 2013: 1 · 2012: 1
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (AM 30516)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- insulin, glucocorticoids, dexamethasone, glucose transport, glucose transporters, adipocytes, insulin sensitivity, plasma membrane, cytochalasin B, actinomycin D
- MeSH terms
- Adipose Tissue, Animals, Biological Transport, Cell Compartmentation, Cell Membrane, Cytochalasin B, Glucocorticoids, Insulin, Methylglucosides, Methylglycosides, Microsomes, Monosaccharide Transport Proteins, 3-O-Methylglucose, Rats, In Vitro Techniques
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