Molecular correlates for maximal oxygen uptake and type 1 fibers
Abstract
Maximal oxygen uptake (Vo(2max)) and the amount of type 1 fibers are interrelated, but the underlying unifying molecular mechanisms are poorly understood. To explore these mechanisms, we related gene expression profiles in skeletal muscle biopsies of 43 age-matched men from published datasets with Vo(2max) and the amount of type 1 fibers and replicated some of the findings in muscle biopsies from 154 young and elderly individuals using real-time PCR. We identified 66 probe sets (genes or expressed sequence tags) positively and 83 probe sets inversely correlated with Vo(2max) and 171 probe sets positively and 217 probe sets inversely correlated with percentage of type 1 fibers in human skeletal muscle. Genes involved in oxidative phosphorylation (OXPHOS) showed high expression in individuals with high Vo(2max), whereas the opposite was not the case in individuals with low Vo(2max). Instead, genes such as AHNAK and BCL6 were associated with low Vo(2max). Also, expression of the OXPHOS genes NDUFB5 and ATP5C1 increased with exercise training and decreased with aging. In contrast, expression of AHNAK in skeletal muscle decreased with exercise training and increased with aging. Eleven genes (NDUFB4, COX5A, UQCRB, ATP5C1, ATP5G3, ETHE1, FABP3, ISCA1, MYST4, C9orf3, and PKIA) were positively correlated with both Vo(2max) and the percentage of type 1 fibers. Vo(2max) closely reflects expression of OXPHOS genes, particularly that of NDUFB5 and ATP5C1, in skeletal muscle, suggesting good muscle fitness. In contrast, a high expression of AHNAK was associated with a low Vo(2max) and poor muscle fitness.
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In humans, maximal oxygen uptake is robustly correlated with OXPHOS gene expression.
“In humans, maximal oxygen uptake is robustly correlated with OXPHOS gene expression (133).”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [133]
Reduced physical fitness is associated with reduced muscle OXPHOS gene expression.
“Reduced physical fitness is associated with reduced muscle OXPHOS gene expression.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [133]
OXPHOS expression can be increased with exercise training, a potent insulin sensitizer.
“Conversely, OXPHOS expression can be increased with exercise training (133, 135), a potent insulin sensitizer.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [133]
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- Authors
- Parikh, H., Nilsson, E., Ling, C., Poulsen, P., Almgren, P., Nittby, H., Eriksson, K.-F., Vaag, A., & Groop, L. C.
- Affiliations
- Lund University (SE)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2008-06
- Volume
- 294
- Issue
- 6
- Pages
- E1152-E1159
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2135667395
- Cited by
- 36
- References
- 44
- Citations by year
- 2025: 1 · 2024: 2 · 2021: 6 · 2020: 1 · 2019: 4 · 2016: 2 · 2015: 3 · 2014: 1 · 2013: 5 · 2012: 1
- FWCI
- 9.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Physical Performance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- VO2max, type I muscle fibers, skeletal muscle gene expression, oxidative phosphorylation, exercise training, aging, muscular fitness, AHNAK, BCL6, cardiorespiratory fitness
- MeSH terms
- Adult, Age Factors, Aged, Biopsy, Exercise Test, Female, Gene Expression Regulation, Humans, Male, Middle Aged, Oxidative Phosphorylation, Oxygen, Oxygen Consumption, Physical Fitness, Twins, Muscle, Skeletal, Muscle Fibers, Slow-Twitch, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis
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