The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Mechanism(s) of butyrate transport in Caco-2 cells: role of monocarboxylate transporter 1
Abstract
The short-chain fatty acid butyrate was readily taken up by Caco-2 cells. Transport exhibited saturation kinetics, was enhanced by low extracellular pH, and was Na+independent. Butyrate uptake was unaffected by DIDS; however, α-cyano-4-hydroxycinnamate and the butyrate analogs propionate and l-lactate significantly inhibited uptake. These results suggest that butyrate transport by Caco-2 cells is mediated by a transporter belonging to the monocarboxylate transporter family. We identified five isoforms of this transporter, MCT1, MCT3, MCT4, MCT5, and MCT6, in Caco-2 cells by PCR, and MCT1 was found to be the most abundant isoform by RNase protection assay. Transient transfection of MCT1, in the antisense orientation, resulted in significant inhibition of butyrate uptake. The cells fully recovered from this inhibition by 5 days after transfection. In conclusion, our data showed that the MCT1 transporter may play a major role in the transport of butyrate into Caco-2 cells.
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Cites
Absorption of Short-Chain Fatty Acids by the Colon
Ruppin, H., Bar-Meir, S., Soergel, K. H., Wood, C. M., & Schmitt, M. G. (1980) · Gastroenterology · doi:10.1016/s0016-5085(19)30508-6 · Cited by 736
PRICE, T. N., JACKSON, N. V., & HALESTRAP, P. A. (1998) · Biochemical Journal · doi:10.1042/bj3290321 · Cited by 361
Mechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon
Mascolo, N., Rajendran, V. M., & Binder, H. J. (1991) · Gastroenterology · doi:10.1016/0016-5085(91)90008-9 · Cited by 155
Kinetic studies on the metabolism of short-chain fatty acids and glucose by isolated rat colonocytes
Clausen, M. R., & Mortensen, P. B. (1994) · Gastroenterology · doi:10.1016/0016-5085(94)90601-7 · Cited by 119
Cited by
den Besten, G., van Eunen, K., Groen, A. K., Venema, K., Reijngoud, D.-J., & Bakker, B. M. (2013) · Journal of Lipid Research · doi:10.1194/jlr.r036012 · Cited by 5,048
Park, J., Kim, M., Kang, S. G., Jannasch, A. H., Cooper, B., Patterson, J., & Kim, C. H. (2015) · Mucosal Immunology · doi:10.1038/mi.2014.44 · Cited by 1,307
Dietary metabolism, the gut microbiome, and heart failure
Tang, W. H. W., Li, D. Y., & Hazen, S. L. (2019) · Nature Reviews Cardiology · doi:10.1038/s41569-018-0108-7 · Cited by 789
Physiology of Intestinal Absorption and Secretion
Kiela, P. R., & Ghishan, F. K. (2016) · Best Practice & Research Clinical Gastroenterology · doi:10.1016/j.bpg.2016.02.007 · Cited by 752
Gut microbiota-derived short-chain Fatty acids, T cells, and inflammation
Kim, C. H., Park, J., & Kim, M. (2014) · Immune Network · doi:10.4110/in.2014.14.6.277 · Cited by 704
Metadata
- Authors
- Hadjiagapiou, C., Schmidt, L., Dudeja, P. K., Layden, T. J., & Ramaswamy, K.
- Venue
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Publisher
- American Physiological Society
- Published
- 2000-10-01
- Volume
- 279
- Issue
- 4
- Pages
- G775-G780
- ISSN
- 0193-1857 (ISSN-L)
- 1522-1547
- OpenAlex
- W2187950387
- Cited by
- 181
- References
- 4
- FWCI
- 1.49
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hadjiagapiou, C., Schmidt, L., Dudeja, P. K., Layden, T. J., & Ramaswamy, K. (2000). Mechanism(s) of butyrate transport in Caco-2 cells: role of monocarboxylate transporter 1. American Journal of Physiology-Gastrointestinal and Liver Physiology. https://doi.org/10.1152/ajpgi.2000.279.4.g775
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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