Cited by 18Cardiac electrophysiology and arrhythmiasPulmonary Hypertension Research and TreatmentsCardiovascular Function and Risk Factors

Pulmonary artery banding alters the expression of Ca2+transport proteins in the right atrium in rabbits

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Gupta, Subash C., Varian, Kenneth D., Bal, Naresh C., Abraham, Jessica L., Periasamy, Muthu, Janssen, Paul M. L.·2009·American Journal of Physiology-Heart and Circulatory Physiology

Abstract

Following pulmonary artery banding (PAB), the contractile function of right ventricle diminishes over time. Subsequently, the right atrium (RA) has to contract against a higher afterload, but it is unknown to what extent ventricular dysfunction has an effect on the atrial contractility. We hypothesized that right ventricular pressure overload may have an affect on atrial contractility and Ca 2+transport protein expression. Therefore, we induced pressure overload of the right ventricle by PAB for 10 wk in rabbits and examined the changes in the expression of Ca 2+transport proteins in the atrium. We demonstrate that PAB significantly decreased the expression of sarco(endo)plasmic reticulum Ca 2+-ATPase (Serca) 2a while expression of Na+/Ca 2+exchanger-1 was significantly upregulated in the RA but not in the left atria of rabbit hearts, indicating that pressure is the major trigger. A decrease in Serca2a expression was concomitant with a significant decrease in sarcolipin (SLN), possibly indicating a compensatory role of SLN. The decreased expression of SLN was unable to completely restore sarcoplasmic reticulum Ca 2+uptake function of Serca2a. Functional contractile assessments in isolated trabeculae showed no difference between PAB- and sham-operated rabbits at 1 Hz but displayed an enhanced force development at higher frequencies and in the presence of isoproterenol, while twitch timing was unaffected. Our results indicate that right ventricular mechanical overload due to PAB affects the expression of the Ca 2+-handling proteins in the RA in rabbits.

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Crossref · 10.1152/ajpheart.00026.2009

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  • Ten weeks of pulmonary artery banding is sufficient to induce right ventricular hypertrophy in rabbits without signs and symptoms of heart failure, and with altered right atrial calcium handling.

    “ten weeks of pulmonary artery banding (PAB) is sufficient to induce hypertrophy in rabbits without signs and symptoms of heart failure in the right ventricle (Varian, et al., 2009) and with altered right atrial calcium handling (Gupta, et al., 2009).”

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Authors
Gupta, S. C., Varian, K. D., Bal, N. C., Abraham, J. L., Periasamy, M., & Janssen, P. M. L.
Affiliations
The Ohio State University (US)
Venue
American Journal of Physiology-Heart and Circulatory Physiology
Published
2009-06
Volume
296
Issue
6
Pages
H1933-H1939
ISSN
  • 0363-6135 (ISSN-L)
  • 1522-1539
OpenAlex
W2130504757
Cited by
18
References
48
Citations by year
2025: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2013: 2 · 2012: 4
FWCI
1.73
Type
journal article
Language
en
Open access
Locations
  • American Journal of Physiology-Heart and Circulatory Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • American Heart Association
  • National Heart, Lung, and Blood Institute (HL-073816, K02HL083957, HL-64140, R01HL073816, HL-083957, R01HL088555, R01HL064140, HL088555)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Pulmonary Hypertension Research and Treatments
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
Keywords
pulmonary artery banding, right atrium, calcium transport proteins, SERCA2a, Na+/Ca2+ exchanger 1, sarcolipin, pressure overload, right ventricular dysfunction, atrial contractility, sarcoplasmic reticulum calcium uptake, isoproterenol, rabbit heart
MeSH terms
Animals, Calcium, Cardiotonic Agents, Heart Atria, Isoproterenol, Male, Myocardial Contraction, Myocardium, Pulmonary Artery, Rabbits, Sarcoplasmic Reticulum, Hypertrophy, Right Ventricular, Sodium-Calcium Exchanger, Sarcoplasmic Reticulum Calcium-Transporting ATPases

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