The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
TRPV4-mediated endothelial Ca2+ influx and vasodilation in response to shear stress
Open accessAbstract
The transient receptor potential vallinoid type 4 (TRPV4) channel has been implicated in the endothelial shear response and flow-mediated dilation, although the precise functions of this channel remain poorly understood. In the present study, we investigated the role of TRPV4 in shear stress-induced endothelial Ca 2+ entry and the potential link between this signaling response and relaxation of small resistance arteries. Using immunohistochemical analysis and RT-PCR, we detected strong expression of TRPV4 protein and mRNA in the endothelium in situ and endothelial cells freshly isolated from mouse small mesenteric arteries. The selective TRPV4 agonist GSK1016790A increased endothelial Ca 2+ and induced potent relaxation of small mesenteric arteries from wild-type (WT) but not TRPV4−/− mice. Luminal flow elicited endothelium-dependent relaxations that involved both nitric oxide and EDHFs. Both nitric oxide and EDHF components of flow-mediated relaxation were markedly reduced in TRPV4−/− mice compared with WT controls. Using a fura-2/Mn 2+ quenching assay, shear was observed to produce rapid Ca 2+ influx in endothelial cells, which was markedly inhibited by the TRPV4 channel blocker ruthenium red and TRPV4-specific short interfering RNA. Flow elicited a similar TRPV4-mediated Ca 2+ entry in HEK-293 cells transfected with TRPV4 channels but not in nontransfected cells. Collectively, these data indicate that TRPV4 may be a potential candidate of mechanosensitive channels in endothelial cells through which the shear stimulus is transduced into Ca 2+ signaling, leading to the release of endothelial relaxing factors and flow-mediated dilation of small resistance arteries.
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Cites
Flow-mediated endothelial mechanotransduction
Davies, P. F. (1995) · Physiological Reviews · doi:10.1152/physrev.1995.75.3.519 · Cited by 2,802
Liedtke, W., Choe, Y., Martí-Renom, M. A., Bell, A. M., Denis, C. S., AndrejŠali, Hudspeth, A. J., Friedman, J. M., & Heller, S. (2000) · Cell · doi:10.1016/s0092-8674(00)00143-4 · Cited by 1,389
Heat-Evoked Activation of the Ion Channel, TRPV4
Güler, A. D., Lee, H., Iida, T., Shimizu, I., Tominaga, M., & Caterina, M. (2002) · The Journal of Neuroscience · doi:10.1523/jneurosci.22-15-06408.2002 · Cited by 1,031
Anandamide and arachidonic acid use epoxyeicosatrienoic acids to activate TRPV4 channels
Watanabe, H., Vriens, J., Prenen, J., Droogmans, G., Voets, T., & Nilius, B. (2003) · Nature · doi:10.1038/nature01807 · Cited by 1,027
OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity
Strotmann, R., Harteneck, C., Nunnenmacher, K., Schultz, G., & Plant, T. D. (2000) · Nature Cell Biology · doi:10.1038/35036318 · Cited by 1,023
Impaired Pressure Sensation in Mice Lacking TRPV4
Suzuki, M., Mizuno, A., Kodaira, K., & Imai, M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m302561200 · Cited by 747
Self-citationHydrogen peroxide is an endothelium-derived hyperpolarizing factor in mice
Matoba, T., Shimokawa, H., Nakashima, M., Hirakawa, Y., Mukai, Y., Hirano, K., Kanaide, H., & Takeshita, A. (2000) · Journal of Clinical Investigation · doi:10.1172/jci10506 · Cited by 722
Watanabe, H., Vriens, J., Suh, S. H., Benham, C. D., Droogmans, G., & Nilius, B. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m208277200 · Cited by 692
Activation of TRPV4 Channels (hVRL-2/mTRP12) by Phorbol Derivatives
Watanabe, H., Davis, J. B., Smart, D., Jerman, J. C., Smith, G. D., Hayes, P., Vriens, J., Cairns, W., Wissenbach, U., Prenen, J., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m200062200 · Cited by 605
Flow activates an endothelial potassium channel to release an endogenous nitrovasodilator.
Cooke, J. P., Rossitch, E., Andon, N. A., Loscalzo, J., & Dzau, V. J. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115481 · Cited by 533
Cited by
Endothelial dysfunction and vascular disease - a 30th anniversary update
Vanhoutte, P. M., Shimokawa, H., Feletou, M., & Tang, E. H. C. (2017) · Acta Physiologica · doi:10.1111/apha.12646 · Cited by 937
Elementary Ca 2+ Signals Through Endothelial TRPV4 Channels Regulate Vascular Function
Sonkusare, S. K., Bonev, A. D., Ledoux, J., Liedtke, W., Kotlikoff, M. I., Heppner, T. J., Hill-Eubanks, D. C., & Nelson, M. T. (2012) · Science · doi:10.1126/science.1216283 · Cited by 552
TRPV4: molecular conductor of a diverse orchestra
White, J. P. M., Cibelli, M., Urban, L., Nilius, B., McGeown, J. G., & Nagy, I. (2016) · Physiological Reviews · doi:10.1152/physrev.00016.2015 · Cited by 405
Transient Receptor Potential Channels in the Vasculature
Earley, S., & Brayden, J. E. (2015) · Physiological Reviews · doi:10.1152/physrev.00026.2014 · Cited by 420
Thorneloe, K. S., Cheung, M., Bao, W., Alsaid, H., Lenhard, S., Jian, M.-Y., Costell, M., Maniscalco-Hauk, K., Krawiec, J. A., Olzinski, A., et al. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3004276 · Cited by 337
Metadata
- Authors
- Mendoza, S. A., Fang, J., Gutterman, D. D., Wilcox, D. A., Bubolz, A. H., Li, R., Suzuki, M., & Zhang, D. X.
- Venue
- American Journal of Physiology-Heart and Circulatory Physiology
- Publisher
- American Physiological Society
- Published
- 2010-02
- Volume
- 298
- Issue
- 2
- Pages
- H466-H476
- ISSN
- 0363-6135 (ISSN-L)
- 1522-1539
- OpenAlex
- W2053627692
- Cited by
- 340
- References
- 48
- FWCI
- 7.41
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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