Left ventricular volumes and function in the embryonic mouse heart
Abstract
This study describes miniaturized technology for the in vivo analysis of the volume and function of the embryonic mouse heart and the application of this technology to study the normal embryonic left ventricle (LV) at two stages of development. With the use of microsurgical techniques, embryos from embryonic day (ED) 10.5 (ED10.5) to ED16 were delivered individually from litters of normal dams, and cardiac visualization was achieved with the use of intravital microscopy by transillumination, with the umbilical circulation intact. At ED10.5-11, the heart could be imaged in color in the intact embryo, whereas at ED12.5 it was necessary to open the chest; at ED13.5-14.5, fluorescent imaging with the use of microinjection of fluorescein-conjugated albumin was necessary to visualize the LV chamber. At ED10.5-11, the LV end-diastolic volumes averaged 0.16 microliter (n = 14), and at ED13.5-14.5, they averaged 0.57 microliter (n = 16). At both ages there was a positive linear relationship between the LV end-diastolic volume and the stroke volume despite substantial variations in individual heart rates, reflecting the relative uniformity of the LV ejection fractions within each age group. The average of the individual ejection fractions was 27.4% at ED10.5-11 and 58.4% at ED13.5-14.5, the latter being within the normal range for the adult rodent heart. These methods will be useful for assessing in vivo cardiac function at ED10.5 and older murine embryos in litters of transgenic or ge…
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In the day 10.5e11 embryonic mouse LV, the reported EF is only about 27%.
“Considering these simplifications, it is noteworthy that in the day 10.5e11 embryonic mouse LV, in which the heart muscle also has a random organization, the reported EF is only about 27% [46], which may be a more appropriate comparison for our hvCOC than an adult EF of 70%.”
From Bioengineering an electro-mechanically functional miniature ventricular heart chamber from human pluripotent stem cells (2018) · cites this paper as [46]
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Cited by 13
- Authors
- Tanaka, N., Mao, L., DeLano, F. A., Sentianin, E. M., Chien, K. R., Schmid-Schonbein, G. W., & Ross, J.
- Affiliations
- University of California San Diego (US)
- Department of Medicine, University of California San Diego, La Jolla92093-0613, USA.
- Venue
- American Journal of Physiology-Heart and Circulatory Physiology
- Publisher
- American Physiological Society
- Published
- 1997-09-01
- Volume
- 273
- Issue
- 3
- Pages
- H1368-H1376
- ISSN
- 0363-6135 (ISSN-L)
- 1522-1539
- OpenAlex
- W2402962791
- Cited by
- 13
- References
- 0
- Citations by year
- 2018: 1
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Heart and Circulatory Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl051549-05, 5r01hl055926-06)
- National Heart, Lung, and Blood Institute (HL 46345, P01HL046345, HL51549, HL55926)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Cardiac Imaging and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Keywords
- left ventricle, left ventricular volume, left ventricular ejection fraction, stroke volume, end-diastolic volume, cardiac function, intravital microscopy, transillumination, embryonic development, transgenic mouse embryos
- MeSH terms
- Animals, Diastole, Female, Embryonic and Fetal Development, Fetal Heart, Gestational Age, Heart Rate, Fetal, Heart Ventricles, Mice, Inbred Strains, Pregnancy, Regression Analysis, Ventricular Function, Left, Coronary Angiography, Microscopy, Video, Mice
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