The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Arsenite stimulates plasma membrane NADPH oxidase in vascular endothelial cells
Abstract
Low-level arsenite treatment of porcine aortic endothelial cells (PAEC) stimulated superoxide accumulation that was attenuated by inhibitors of NAD(P)H oxidase. To demonstrate whether arsenite stimulated NADPH oxidase, intact PAEC were treated with arsenite for up to 2 h and membrane fractions were prepared to measure NADPH oxidase activity. Arsenite (5 μM) stimulated a twofold increase in activity by 1 h, which was inhibited by the oxidase inhibitor diphenyleneiodonium chloride. Direct treatment of isolated membranes with arsenite had no effect. Analysis of NADPH oxidase components revealed that p67 phox localized exclusively to membranes of both control and treated cells. In contrast, cytosolic Rac1 translocated to the membrane fractions of cells treated with arsenite or angiotensin II but not with tumor necrosis factor. Immunodepletion of p67 phox blocked oxidase activity stimulated by all three compounds. However, depleting Rac1 inhibited responses only to arsenite and angiotensin II. These data demonstrate that stimulus-specific activation of NADPH oxidase in endothelial cells was the source of reactive oxygen in endothelial cells after noncytotoxic arsenite exposure.
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Görlach, A., Brandes, R. P., Nguyen, K., Amidi, M., Dehghani, F., & Busse, R. (2000) · Circulation Research · doi:10.1161/01.res.87.1.26 · Cited by 598 · cited 2×
“(11) used gp91(2/2) mice to demonstrate that the endothelial NADPH oxidase is a major source of oxygen radical generation in the arterial wall.”
BackgroundPagano, P. J., Clark, J. K., Cifuentes-Pagano, M. E., Clark, S. M., Callis, G. M., & Quinn, M. T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.26.14483 · Cited by 455
Two cytosolic neutrophil oxidase components absent in autosomal chronic granulomatous disease
Volpp, B. D., Nauseef, W. M., & Clark, R. A. (1988) · Science · doi:10.1126/science.2848318 · Cited by 407
An NADPH oxidase superoxide-generating system in the rabbit aorta
Pagano, P. J., Ito, Y., Tornheim, K., Gallop, P. M., Tauber, A. I., & Cohen, R. A. (1995) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1995.268.6.h2274 · Cited by 302
Cited by
Arsenic Exposure and Toxicology: A Historical Perspective
Hughes, M. F., Beck, B. D., Chen, Y., Lewis, A. S., & Thomas, D. J. (2011) · Toxicological Sciences · doi:10.1093/toxsci/kfr184 · Cited by 1,312
Metal-induced oxidative stress and signal transduction
Leonard, S. S., Harris, G. K., & Shi, X. (2004) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.09.010 · Cited by 663
Jiang, F., Zhang, Y., & Dusting, G. J. (2011) · Pharmacological Reviews · doi:10.1124/pr.110.002980 · Cited by 572
Mechanism of arsenic carcinogenesis: an integrated approach
Rossman, T. G. (2003) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/j.mrfmmm.2003.07.009 · Cited by 559
Arsenic in the aetiology of cancer
Tapio, S., & Grosche, B. (2006) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2006.02.001 · Cited by 334
Metadata
- Authors
- Smith, K. R., Klei, L. R., & Barchowsky, A.
- Venue
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Publisher
- American Physiological Society
- Published
- 2001-03-01
- Volume
- 280
- Issue
- 3
- Pages
- L442-L449
- ISSN
- 1040-0605 (ISSN-L)
- 1522-1504
- OpenAlex
- W2183232481
- Cited by
- 189
- References
- 4
- FWCI
- 3.13
- Type
- journal article
- Language
- en
- Open access
- closed
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