Cited by 302Nitric Oxide and Endothelin EffectsNeutrophil, Myeloperoxidase and Oxidative MechanismsRedox biology and oxidative stress

An NADPH oxidase superoxide-generating system in the rabbit aorta

Pagano, P. J., Ito, Y., Tornheim, K., Gallop, P. M., Tauber, A. I., Cohen, R. A.·1995·American Journal of Physiology-Heart and Circulatory Physiology

Abstract

Superoxide anion can modulate vascular smooth muscle tone and potentially affect the growth response in vascular disease. The present studies were undertaken to characterize the source of superoxide in rabbit aorta. Rings of aorta (5 mm) were incubated in physiological salt solution (PSS) for 30 min at 37 degrees C in the presence of 10 mM diethyldithiocarbamate (DDC) with or without inhibitors of superoxide-generating systems. Rings were then placed in PSS containing 250 microM lucigenin at 37 degrees C in the presence or absence of inhibitors, and changes in amounts of superoxide were determined by measuring chemiluminescence (units). The inhibitors of xanthine oxidase, oxypurinol (300 microM), and of mitochondrial NADH dehydrogenase, rotenone (50 microM), had no significant effect on superoxide levels. An inhibitor of NADPH oxidase, iodonium thiophen, caused a concentration-dependent inhibition of superoxide anion (12.49 +/- 1.48 vs 5.27 +/- 1.81 and 2.30 +/- 0.36 units, control vs 7 microM and 70 microM iodonium thiopen, respectively). A structurally related iodonium compound, diphenyleneiodonium (20 microM), caused a 78% reduction in basal and DDC-evoked superoxide levels. In the presence or absence of DDC, exogenous administration of NADPH (10 microM-1 mM), but not NADP (1 mM), elicited a concentration-dependent rise in superoxide levels that was inhibited by iodonium thiophen. Particulate fractions of whole aortic tissue exhibited NADPH-dependent superoxide production that was inhibited by 1 microM diphenyleneiodonium.(ABSTRACT TRUNCATED AT 250 WORDS)

Integrity signals

Registry

Crossref · 10.1152/ajpheart.1995.268.6.h2274

Cites

24 of 24 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • A large number of cytokines and hormones induce NOX2 expression, including angiotensin II, thrombin, and TNF-α.

    “A large number of cytokines and hormones have been shown to induce NOX2 expression, including angiotensin II (658, 714, 802), thrombin (725), and TNF-α (275, 964).”
  • NOX2 expression has been reported in other cell types including endothelial cells and adventitial fibroblasts.

    “NOX2 expression has been also reported in other cell types including endothelial cells and adventitial fibroblasts (376, 382, 657, 714, 715, 736, 876) (FIGURE 9, Table 5).”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Pagano, P. J., Ito, Y., Tornheim, K., Gallop, P. M., Tauber, A. I., & Cohen, R. A.
Affiliations
Robert Dawson Evans Department of Clinical Research, UniversityMedical Center, Boston 02118, USA.
Venue
American Journal of Physiology-Heart and Circulatory Physiology
Published
1995-06-01
Volume
268
Issue
6
Pages
H2274-H2280
ISSN
  • 0363-6135 (ISSN-L)
  • 1522-1539
OpenAlex
W2219200263
Cited by
302
References
24
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 6 · 2018: 6 · 2017: 4 · 2016: 3 · 2015: 7 · 2014: 7 · 2013: 6 · 2012: 5
FWCI
7.50
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Heart and Circulatory Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p50hl047124-04, 5r01hl038731-08)
  • National Heart, Lung, and Blood Institute (HL-38731, R01HL031607, HL 47124, HL31607)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
NADPH oxidase, superoxide, rabbit aorta, vascular smooth muscle, diphenyleneiodonium, diethyldithiocarbamate, chemiluminescence, xanthine oxidase, mitochondrial NADH dehydrogenase, vascular tone
MeSH terms
Animals, Aorta, Thoracic, Ditiocarb, Kinetics, Male, Muscle, Smooth, Vascular, NADH, NADPH Oxidoreductases, NADP, Onium Compounds, Oxypurinol, Rabbits, Rotenone, Subcellular Fractions, Superoxides, Thiophenes, NADPH Oxidases, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review