Hypotonic induction of SGK1 and Na+transport in A6 cells
Open accessAbstract
Serum and glucocorticoid-regulated kinase-1 (SGK1) is a serine-threonine kinase that is regulated at the transcriptional level by numerous regulatory inputs, including mineralocorticoids, glucocorticoids, follicle-stimulating hormone, and osmotic stress. In the distal nephron, SGK1 is induced by aldosterone and regulates epithelial Na+ channel-mediated transepithelial Na+ transport. In other tissues, including liver and shark rectal gland, SGK1 is regulated by hypertonic stress and is thought to modulate epithelial Na+ channel- and Na+-K+-2Cl- cotransporter-mediated Na+ transport. In this report, we examined the regulation of SGK1 mRNA and protein expression and Na+ currents in response to osmotic stress in A6 cells, a cultured cell line derived from Xenopus laevis distal nephron. We found that in contrast to hepatocytes and rectal gland cells, hypotonic conditions stimulated SGK1 expression and Na+ transport in A6 cells. Moreover, a correlation was found between SGK1 induction and the later phase of activation of Na+ transport in response to hypotonic treatment. When A6 cells were pretreated with an inhibitor of phosphatidylinositol 3-kinase (PI3K), Na+ transport was blunted and only inactive forms of SGK1 were expressed. Surprisingly, these results demonstrate that both hypertonic and hypotonic stimuli can induce SGK1 gene expression in a cell type-dependent fashion. Moreover, these data lend support to the view that SGK1 contributes to the defense of extracellular fluid vo…
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Both hyperosmotic and hypoosmotic stimuli can induce SGK1 gene expression in a cell-type-dependent manner.
“Interestingly, both hyperosmotic and hypoosmotic stimuli can induce the expression of the SGK1 gene in a cell-type-dependent manner”
From Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain (2024) · cites this paper as Rozansky et al., 2002
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Lang, F., Böhmer, C., Palmada, M., Seebohm, G., Strutz-Seebohm, N., & Vallon, V. (2006) · Physiological Reviews · doi:10.1152/physrev.00050.2005 · Cited by 689 · see citing works
PANCHAPAKESAN, U., POLLOCK, C., & SAAD, S. (2009) · Nephrology · doi:10.1111/j.1440-1797.2009.01089.x · Cited by 25 · see citing works
Chen, B.-Y., Huang, C.-C., Lv, X.-F., Zheng, H.-Q., Zhang, Y.-J., Sun, L., Wang, G.-L., Ma, M.-M., & Guan, Y.-Y. (2020) · Acta Pharmacologica Sinica · doi:10.1038/s41401-020-0357-y · Cited by 18 · see citing works
Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain
Howard, P. G., Zou, P., Zhang, Y., Huang, F., Tesic, V., Wu, C. Y.-C., & Lee, R. H.-C. (2024) · Experimental Neurology · doi:10.1016/j.expneurol.2024.114973 · Cited by 13 · see citing works
- Authors
- Rozansky, D. J., Wang, J., Doan, N., Purdy, T., Faulk, T., Bhargava, A., Dawson, K., & Pearce, D.
- Affiliations
- Division of Nephrology, Department of Pediatrics, and
- University of California, San Francisco (US)
- Oregon Health & Science University (US)
- Venue
- American Journal of Physiology-Renal Physiology
- Publisher
- American Physiological Society
- Published
- 2002-07-01
- Volume
- 283
- Issue
- 1
- Pages
- F105-F113
- ISSN
- 1522-1466 (ISSN-L)
- 1931-857X
- OpenAlex
- W2115774544
- Cited by
- 65
- References
- 0
- Citations by year
- 2024: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 1
- FWCI
- 2.43
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- American Journal of Physiology-Renal Physiology (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK 56695, R01-DK-51151, R01DK051151, R01DK056695)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Keywords
- SGK1, hypotonic stress, osmotic stress, sodium transport, A6 cells, distal nephron, epithelial Na+ channel, Na+-K+-2Cl- cotransporter, PI3K signaling, gene expression regulation, cell type-specific responses, extracellular volume, Xenopus laevis
- MeSH terms
- Phosphoinositide-3 Kinase Inhibitors, Serum-Glucocorticoid Regulated Kinases, Animals, Cell Line, Chromones, Enzyme Inhibitors, Hypertonic Solutions, Hypotonic Solutions, Morpholines, Nuclear Proteins, Osmotic Pressure, RNA, Messenger, Sodium, Transcription, Genetic, Water-Electrolyte Balance, Sodium Channels, Gene Expression Regulation, Enzymologic, Protein Serine-Threonine Kinases, Immediate-Early Proteins, Epithelial Sodium Channels
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