The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Mouse model of ischemic acute kidney injury: technical notes and tricks
Open accessAbstract
Renal ischemia-reperfusion leads to acute kidney injury (AKI), a major kidney disease associated with an increasing prevalence and high mortality rates. A variety of experimental models, both in vitro and in vivo, have been used to study the pathogenic mechanisms of ischemic AKI and to test renoprotective strategies. Among them, the mouse model of renal clamping is popular, mainly due to the availability of transgenic models and the relatively small animal size for drug testing. However, the mouse model is generally less stable, resulting in notable variations in results. Here, we describe a detailed protocol of the mouse model of bilateral renal ischemia-reperfusion. We share the lessons and experiences gained from our laboratory in the past decade. We further discuss the technical issues that account for the variability of this model and offer relevant solutions, which may help other investigators to establish a well-controlled, reliable animal model of ischemic AKI.
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Citations
Cites
Regulation of mitochondrial dynamics in acute kidney injury in cell culture and rodent models
Brooks, C., Wei, Q., Cho, S.-G., & Dong, Z. (2009) · Journal of Clinical Investigation · doi:10.1172/jci37829 · Cited by 735 · cited 1×
“We routinely conduct H&E staining to grade tubular damage (0, no damage; 1, 0–25% damaged tubules; 2, 25–50% damaged tubules; 3, 50–75% damaged tubules; 4, 75% damaged tubules) (10, 72).”
MethodSelf-citationPositive effect of the induction of p21WAF1/CIP1 on the course of ischemic acute renal failure
Megyesi, J., Andrade, L., Vieira, J. M., Safirstein, R. L., & Price, P. M. (2001) · Kidney International · doi:10.1046/j.1523-1755.2001.00044.x · Cited by 153
Cited by
Non-Transgenic Mouse Models of Kidney Disease
Rabe, M., & Schaefer, F. (2016) · Nephron · doi:10.1159/000445171 · Cited by 1,922
Tang, C., Han, H., Yan, M., Zhu, S., Liu, J., Liu, Z., He, L., Tan, J., Liu, Y., Liu, H., et al. (2018) · Autophagy · doi:10.1080/15548627.2017.1405880 · Cited by 309
Self-citationRenal ischemia/reperfusion injury: an insight on in vitro and in vivo models
Shiva, N., Sharma, N., Kulkarni, Y. A., Mulay, S. R., & Gaikwad, A. B. (2020) · Life Sciences · doi:10.1016/j.lfs.2020.117860 · Cited by 177
Fu, H., Zhou, D., Zhu, H., Liao, J., Lin, L., Hong, X., Hou, F. F., & Liu, Y. (2019) · Kidney International · doi:10.1016/j.kint.2018.11.043 · Cited by 65
mRNA lipid nanoparticle formulation, characterization and evaluation
Ma, Y., VanKeulen-Miller, R., & Fenton, O. S. (2025) · Nature Protocols · doi:10.1038/s41596-024-01134-4 · Cited by 54
Metadata
- Authors
- Wei, Q., & Dong, Z.
- Venue
- American Journal of Physiology-Renal Physiology
- Publisher
- American Physiological Society
- Published
- 2012-12-01
- Volume
- 303
- Issue
- 11
- Pages
- F1487-F1494
- ISSN
- 1522-1466 (ISSN-L)
- 1931-857X
- OpenAlex
- W2160807970
- Cited by
- 303
- References
- 2
- FWCI
- 6.55
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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