Lipolytic response of fat cells to catecholamines in sedentary and exercise-trained women
Abstract
It has been shown that adipose tissue lipolytic activity is increased in endurance-trained subjects. In women, adipose tissue is extensive and it was thought interesting to confirm that endurance training increases the capacity of female adipose tissue to mobilize lipids, and moreover to more fully understand the mechanisms involved. So, biopsies of fat were obtained from the periumbilical region of 13 trained female runners (T) and 17 sedentary women (S) and the in vitro response to catecholamines of the collagenase-isolated fat cells was studied. Glycerol release, chosen as adipocyte lipolysis indicator, was measured by bioluminescence for various epinephrine and norepinephrine concentrations. In both groups, these substances provoked an increase in lipolysis, but the response was significantly higher in T. In both groups, isoproterenol increased the lipolytic activity above basal concentrations at 10(-8) M and above. Lipolytic activity in T was significantly higher (P less than 0.01) than the S control at 10(-7) M and above. Epinephrine plus propranolol decreased lipolysis in both groups, but at 10(-5) M, lipolytic activity was significantly lower in S than in T (P less than 0.05). It is concluded that in female subjects, endurance training increases the sensitivity of subcutaneous abdominal adipose tissue to the lipolytic action of catecholamines; this effect seems to be related both to a decreased efficiency of the alpha 2-adrenergic pathway and to an increased efficiency of the beta-adrenergic pathway. This latter effect seems to take place at a step beyond the receptor-adenylate cyclase system in the lipolytic cascade.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Lipolytic activity within adipose tissue and intramuscular fat is highly active during moderate-intensity endurance exercise by the action of catecholamines, leading to elevated circulating glycerol levels.
“Lipolytic activity within adipose tissue and intramuscular fat is highly active during moderate-intensity endurance exercise by the action of catecholamines, leading to elevated circulating glycerol levels [134,188].”
From Regulation of Energy Substrate Metabolism in Endurance Exercise (2021) · cites this paper as [188]
Cited by
Sorted by citations
Catecholamines and the Effects of Exercise, Training and Gender
Zouhal, H., Jacob, C., Delamarche, P., & Gratas-Delamarche, A. (2008) · Sports Medicine · doi:10.2165/00007256-200838050-00004 · Cited by 553 · see citing works
High-Intensity Intermittent Exercise and Fat Loss
Boutcher, S. H. (2010) · Journal of Obesity · doi:10.1155/2011/868305 · Cited by 559 · see citing works
Physical Activity and Exercise in the Regulation of Human Adipose Tissue Physiology
Thompson, D., Karpe, F., Lafontan, M., & Frayn, K. N. (2012) · Physiological Reviews · doi:10.1152/physrev.00012.2011 · Cited by 403 · see citing works
“Cross-sectional studies indicate that isolated adipocytes from active men and women examined ex vivo have a greater response to lipolytic agents per cell or per gram of lipid than sedentary controls (42, 43, 169, 204).”
BackgroundLipid metabolism during endurance exercise
Horowitz, J. F., & Klein, S. (2000) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/72.2.558s · Cited by 397 · see citing works
You, T., Berman, D. M., Ryan, A. S., & Nicklas, B. J. (2004) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-031310 · Cited by 228 · see citing works
Fatty acid mobilization from adipose tissue during exercise
Horowitz, J. F. (2003) · Trends in Endocrinology and Metabolism · doi:10.1016/s1043-2760(03)00143-7 · Cited by 227 · see citing works
Effects of exercise intensity and training on lipid metabolism in young women.
Friedlander, A., Casazza, G., Horning, M., Buddinger, T. F., & Brooks, G. (1998) · American Journal of Physiology. Endocrinology and Metabolism · doi:10.1152/ajpendo.1998.275.5.e853 · Cited by 182 · see citing works
“However, results of studies comparing men and women suggest that training may lead to better subcutaneous abdominal lipid mobilization in women (6) and that in women the increased mobilization results from both an upregulation of the b-adrenergic stimulation pathway of FFA lipolysis and a downregulation of the inhibit…”
BackgroundInfluentialRegulation of Energy Substrate Metabolism in Endurance Exercise
Alghannam, A. F., Ghaith, M. M., & Alhussain, M. H. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph18094963 · Cited by 104 · see citing works
Interactions between insulin and exercise
Richter, E. A., Sylow, L., & Hargreaves, M. (2021) · Biochemical Journal · doi:10.1042/bcj20210185 · Cited by 99 · see citing works
Adrenergic receptor function in fat cells1-3
Arner, P. (1992) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/55.1.228s · Cited by 82 · see citing works
Nicklas, B. J., Rogus, E. M., & Goldberg, A. P. (1997) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1997.273.1.e149 · Cited by 76 · see citing works
Endurance training changes in lipolytic responsiveness of obese adipose tissue
de Glisezinski, I., Crampes, F., Harant, I., Berlan, M., Hejnová, J., Langin, D., Rivière, D., & Štich, V. (1998) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1998.275.6.e951 · Cited by 72 · see citing works
“agreement with previous data (24, 29) in crosssectional studies but in disagreement with others (12, 20).”
ResultSelf-citationStallknecht, B. M., Simonsen, L., Bülow, J., Vinten, J., & Galbo, H. (1995) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1995.269.6.e1059 · Cited by 72 · see citing works
van Aggel‐Leijssen, D. P., Saris, W. H. M., Hul, G. B. J., & van Baak, M. A. (2001) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.3.523 · Cited by 66 · see citing works
Enevoldsen, L. H., Stallknecht, B. M., Langfort, J., Petersen, L. N., Holm, C., Ploug, T., & Galbo, H. (2001) · The Journal of Physiology · doi:10.1111/j.1469-7793.2001.t01-1-00871.x · Cited by 64 · see citing works
“…with previous in vitro studies in which it was shown that training increases the adrenaline-stimulated lipolysis of periumbilical adipocytes in humans (Crampes et al. 1986; Riviere et al. 1989) and of parametrial and epididymal adipocytes in rats (Bukowiecki et al. 1980; Crampes et al. 1986).”
BackgroundBryner, R. W., Toffle, R. C., Ullrich, I. H., & YEATER, R. A. (1997) · Journal of the American College of Nutrition · doi:10.1080/07315724.1997.10718651 · Cited by 61 · see citing works
Influence of Acute and Chronic Exercise on Abdominal Fat Lipolysis: An Update
Laurens, C., de Glisezinski, I., Larrouy, D., Harant, I., & Moro, C. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.575363 · Cited by 42 · see citing works
Effect of training on GH and IGF-1 responses to a submaximal exercise in football players
Mejri, S., Bchir, F., Rayana, M. B., Hamida, J. B., & Slama, C. B. (2005) · European Journal of Applied Physiology · doi:10.1007/s00421-005-0007-6 · Cited by 45 · see citing works
Regulation of Extramuscular Fuel Sources During Exercise
Wasserman, D. H., & Cherrington, A. D. (1996) · Comprehensive physiology · doi:10.1002/cphy.cp120123 · Cited by 41 · see citing works
Exercise prescription for weight management
Hills, A. P., & Byrne, N. M. (1998) · Proceedings of The Nutrition Society · doi:10.1079/pns19980015 · Cited by 37 · see citing works
Lipid Mobilization and Utilization
B�low, J. (2003) · KARGER eBooks · doi:10.1159/000074369 · Cited by 36 · see citing works
de Glisezinski, I., Marion-Latard, F., Crampes, F., Berlan, M., Hejnová, J., Cottet-Émard, J. M., Štich, V., & Rivière, D. (2001) · Journal of Applied Physiology · doi:10.1152/jappl.2001.91.4.1760 · Cited by 30 · see citing works
“It was indicated previously, in vitro, that endurance training induces a decrease of the antilipolytic a2-mediated action of epinephrine, both in longitudinal studies (10) and in cross-sectional studies (28).”
BackgroundSelf-citationHorowitz, J. F., Braudy, R. J., Martin, W. H., & Klein, S. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.277.2.e325 · Cited by 28 · see citing works
“Several studies have reported that the lipolytic response to epinephrine was more than twofold greater in adipocytes obtained from endurance-trained compared with untrained subjects (5, 23).”
BackgroundResultInfluentialPistor, K., Sepa-Kishi, D. M., Hung, S., & Ceddia, R. B. (2014) · Adipocyte · doi:10.4161/21623945.2014.955423 · Cited by 25 · see citing works
“In fact, there are studies in which the volume of adipocytes in endurance-trained subjects is significantly lower than sedentary controls and lipolysis is expressed per amount of lipid in each incubation.(8,9) In such cases, lipolysis values in the endurance trained condition could derive from a much higher number of…”
BackgroundThe effect of exercise training on β-adrenergic stimulation of fat metabolism in obese men
van Aggel-Leijssen, D., Saris, W. H. M., Homan, M., & van Baak, M. A. (2001) · International Journal of Obesity · doi:10.1038/sj.ijo.0801470 · Cited by 25 · see citing works
Effect of exercise on lipolysis in adipose tissue
Polak, J., Bajzová, M., & Štich, V. (2008) · Future Lipidology · doi:10.2217/17460875.3.5.557 · Cited by 20 · see citing works
Harant, I., Marion-Latard, F., Crampes, F., de Glisezinski, I., Berlan, M., Štich, V., & Rivière, D. (2002) · International Journal of Obesity · doi:10.1038/sj.ijo.0802072 · Cited by 16 · see citing works
Self-citationEpinephrine responsiveness is reduced in livers from trained mice
Dibe, H. A., Townsend, L. K., McKie, G. L., & Wright, D. C. (2020) · Physiological Reports · doi:10.14814/phy2.14370 · Cited by 15 · see citing works
“…EPI VEH EPI SED TR PDE4B Ponceau 83 kDa ~100 kDa 10 of 14 | DIBE Et al. bout of exercise increases catecholamine-induced lipolysis (Crampes, Beauville, Riviere, Garrigues, & Lafontan, 1988; Riviere et al., 1989; Wahrenberg, Bolinder, & Arner, 1991; Wahrenberg, Engfeldt, Bolinder, & Arner, 1987).”
BackgroundInfluentialEffect of endurance training on muscle fat metabolism during prolonged exercise:
Smekal, G., von DUVILLARD, S. P., Pokan, R., Tschan, H., Baron, R., Hofmann, P., Wonisch, M., & Bachl, N. (2003) · Nutrition · doi:10.1016/s0899-9007(03)00171-0 · Cited by 15 · see citing works
Stallknecht, B. M. (2004) · PubMed · Cited by 15 · see citing works
Mittendorfer, B., & Klein, S. (2003) · Nutrition Research Reviews · doi:10.1079/nrr200357 · Cited by 13 · see citing works
“…in adipocytes obtained from endurance-trained subjects than in those from untrained subjects (Despres et al. 1984; Crampes et al. 1986, 1989; Riviere et al. 1989), lipolytic activity is the same or slightly lower in adipocytes from endurance-trained subjects at physiological adrenaline…”
BackgroundLipolytic sensitivity to catecholamines in patients with human immunodeficiency virus infection
Heijligenberg, R., Romijn, J. A., Klein, S., Endert, E., & Sauerwein, H. P. (1997) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/66.3.633 · Cited by 10 · see citing works
Métabolisme lipidique et exercice musculaire chez le sujet obèse
Garrigue, E., de Glisezinski, I., Harant, I., Moro, C., Pillard, F., Crampes, F., & Rivière, D. (2006) · Science & Sports · doi:10.1016/j.scispo.2005.06.011 · Cited by 7 · see citing works
Self-citationAdipose Tissue Lipid Metabolism During Exercise
Horowitz, J. F. (2022) · Physiology in health and disease · doi:10.1007/978-3-030-94305-9_7 · Cited by 5 · see citing works
The Effect of Combined Aerobic and Strength Training on a Weight Loss and Metabolic Profile
Muhammad, H. F. L., Sulistyoningrum, D. C., Apriliana, N. L., Putri, S. E., Larasati, A., Tsani, A. F. A., Kandarina, B. J. I., Sofro, Z. M., & Huriyati, E. (2017) · Topics in Clinical Nutrition · doi:10.1097/tin.0000000000000100 · Cited by 5 · see citing works
Yamashita, Y., Koyama, K., Kaya, M., Ishigaki, T., Tsujita, J., Hori, S., & Oku, Y. (2005) · Journal of PHYSIOLOGICAL ANTHROPOLOGY and Applied Human Science · doi:10.2114/jpa.24.535 · Cited by 3 · see citing works
Place des lipides dans l’alimentation du sportif
Bigard, A. X., Letout, A., Simler, N., Banzet, S., & Koulmann, N. (2004) · Science & Sports · doi:10.1016/j.scispo.2004.01.001 · Cited by 3 · see citing works
Modulations physiologiques et physiopathologiques de la lipolyse chez l’homme
Moro, C. (2012) · Cahiers de Nutrition et de Diététique · doi:10.1016/j.cnd.2012.08.001 · Cited by 2 · see citing works
Duclos, M. (2004) · Science & Sports · doi:10.1016/s0765-1597(03)00189-8 · Cited by 1 · see citing works
Harant, I., Marion-Latard, F., de Glisezinski, I., Pillard, F., Crampes, F., & Rivière, D. (2007) · Elsevier eBooks · doi:10.1016/b978-2-294-08871-1.50003-9
Self-citationWaldron, M. (2013) · ChesterRep (University of Chester) · doi:10.1080/17461391.2012.714405
Guezennec, C. Y., & Jouanin, J. (1997) · Revue Française des Laboratoires · doi:10.1016/s0338-9898(97)80207-7
EFFECTS OF ENDURANCE TRAINING UNDER HYPEROXIA ON CARBOHYDRATE AND FAT METABOLISM IN RATS
Fujise, T., Uchiyama, S., Terao, T., et al. (1991) · 体力科學 · doi:10.7600/jspfsm1949.40.208
Insulin Sensitivity is Enhanced by CGMP-mediated MAPK Inhibition in Rat Adipocytes
Thomas, G. (2010) · Library and Archives Canada (Government of Canada)
Lonac, M. C., Bell, C., Hickey, M. S., Nelson-Ceschin, T. L., & Melby, C. L. (2007) · Digital Collections of Colorado (Colorado State University) · doi:10.25675/3.022168
Joaquim, A. G. (2019) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.17.2019.tde-06092019-141545
Regulation of Extramuscular Fuel Sources During Exercise
Wasserman, D. H., & Cherrington, A. D. (1995) · Comprehensive physiology · doi:10.1002/j.2040-4603.1995.tb01720.x
“This concept is supported by the demonstration that dibutyryl cAMP-stimulated (34, 244) and cAMP-stimulated (139) lipolysis in normal and permeabilized adipocytes, respectively, is increased in the trained state.”
- Authors
- Riviere, D., Crampes, F., Beauville, M., & Garrigues, M.
- Affiliations
- Laboratoire de Physiologie Toulouse-Purpan, France.
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 1989-01-01
- Volume
- 66
- Issue
- 1
- Pages
- 330-335
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W141125801
- Cited by
- 50
- References
- 0
- Citations by year
- 2022: 2 · 2021: 2 · 2020: 2 · 2019: 1 · 2017: 2 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 0.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- adipocyte lipolysis, catecholamines, endurance training, epinephrine, norepinephrine, isoproterenol, propranolol, beta-adrenergic signaling, subcutaneous adipose tissue, glycerol release, sedentary women
- MeSH terms
- Adipose Tissue, Adult, Catecholamines, Bucladesine, Epinephrine, Female, Humans, Isoproterenol, Lipolysis, Norepinephrine, Physical Education and Training, Propranolol
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
