Muscle function and protein metabolism after initiation of eccentric contraction-induced injury
Abstract
This study was designed to determine the relationship between skeletal muscle function and protein metabolism after initiation of eccentric contraction-induced injury. Mouse anterior crural muscles were injured in vivo, and then either immediately or 3, 6, 24, 48, 72, 120, or 336 h after injury muscles were isolated and studied for indexes of muscle function, injury, phagocyte infiltration, and protein metabolism. A group of mice were administered anti-polymorphonuclear cell and anti-macrophage antisera in an attempt to reduce phagocytic infiltration into injured muscle. Force production in extensor digitorum longus muscles was reduced 55% immediately after injury induction and did not recover significantly until 120 h postinjury (28% below baseline). However, rates of protein degradation were not elevated until 48 h postinjury (60% above normal) and were not correlated with the changes in force production (r = -0.37; P = 0.24). Phagocytic infiltration was evident 24-120 h postinjury and was correlated with the elevated protein degradation rates (r = 0.75; P < 0.01). Protein synthesis rates began to increase approximately 48 h after injury was induced and were elevated by 83% 5 days postinjury. Fourteen days after injury, muscle protein degradation and synthesis rates had returned to normal, as well as specific force production, and phagocytic infiltration was not detected. However, muscle mass, protein content, and absolute force production were lower than normal. Antisera-treated mice were rendered neutropenic, but there was no difference in any variable measured between muscles from these mice and muscles from normal mice.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Functional capacity is significantly impaired immediately and for several days after 150 eccentric contractions.
“Here in this study, as with many others (34, 35, 42–44), we reaffirm that functional capacity is significantly impaired immediately, and for several days after, 150 eccentric contractions.”
From Phytoecdysteroids Accelerate Recovery of Skeletal Muscle Function Following in vivo Eccentric Contraction-Induced Injury in Adult and Old Mice (2021) · cites this paper as [34]
Alterations in skeletal muscle protein metabolism may be responsible for the remaining strength deficits in the 1-2 weeks after eccentric damage.
“Furthermore, there is sufficient evidence that alterations in skeletal muscle protein metabolism may be responsible for the remaining strength deficits in the 1–2 weeks after eccentric damage (34, 42).”
From Phytoecdysteroids Accelerate Recovery of Skeletal Muscle Function Following in vivo Eccentric Contraction-Induced Injury in Adult and Old Mice (2021) · cites this paper as [34]
There is a dissociation between muscle function and histological markers of muscle damage that disputes the causal relationship that disruption of sarcomeric structures causes loss of muscle function after eccentric damage.
“However, there is a dissociation between muscle function and histological markers of muscle damage that disputes the latter causal relationship.”
From Phytoecdysteroids Accelerate Recovery of Skeletal Muscle Function Following in vivo Eccentric Contraction-Induced Injury in Adult and Old Mice (2021) · cites this paper as [34]
The greatest declines in muscle function occur immediately following eccentric contraction-induced injury, yet markers of muscle damage are not fully apparent until 1-2 days after injury.
“Recall that the greatest declines in muscle function occur immediately following eccentric contraction-induced injury, yet markers of muscle damage are not fully apparent until 1–2 days after injury (34, 47, 52).”
From Phytoecdysteroids Accelerate Recovery of Skeletal Muscle Function Following in vivo Eccentric Contraction-Induced Injury in Adult and Old Mice (2021) · cites this paper as [34]
Force-producing capabilities of muscle usually return to baseline between 2 and 4 weeks post-eccentric contraction-induced injury.
“The force-producing capabilities of the muscle usually return to baseline (pre-injury) levels between 2 and 4 weeks post-eccentric contraction-induced injury (34, 42, 44, 46, 47).”
From Phytoecdysteroids Accelerate Recovery of Skeletal Muscle Function Following in vivo Eccentric Contraction-Induced Injury in Adult and Old Mice (2021) · cites this paper as [34]
The novel observation that 20E treatment fully recovers skeletal muscle function in just 7 days after eccentric damage is much earlier than other studies using similar eccentric damage protocols in mice.
“The novel observation that 20E treatment fully recovers skeletal muscle function in just 7 days after eccentric damage is much earlier than other studies using similar eccentric damage protocols in mice (34, 35, 42, 47).”
From Phytoecdysteroids Accelerate Recovery of Skeletal Muscle Function Following in vivo Eccentric Contraction-Induced Injury in Adult and Old Mice (2021) · cites this paper as [34]
The citation lists haven’t been fetched yet
References 31 · Cited by 170
- Authors
- Lowe, D. A., Warren, G. L., Ingalls, C. P., Boorstein, D. B., & Armstrong, R. B.
- Affiliations
- Texas A&M University (US)
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 1995-10-01
- Volume
- 79
- Issue
- 4
- Pages
- 1260-1270
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2107614370
- Cited by
- 170
- References
- 31
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 1 · 2023: 5 · 2022: 5 · 2021: 7 · 2020: 6 · 2019: 6 · 2018: 5 · 2017: 3 · 2016: 8 · 2015: 4 · 2014: 6 · 2013: 3 · 2012: 8
- FWCI
- 1.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ar042761-03)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR-42761)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- skeletal muscle function, muscle protein metabolism, protein degradation, protein synthesis, neutrophils, macrophages, muscle force production, extensor digitorum longus, muscle mass, antisera
- MeSH terms
- Animals, DNA, Female, Isometric Contraction, Kinetics, L-Lactate Dehydrogenase, Macrophages, Mice, Inbred ICR, Muscle Contraction, Muscle Proteins, Peroxidase, Neutrophils, Phagocytosis, RNA, Muscle, Skeletal, Muscle Fibers, Skeletal, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
