Inflammatory Cells and Apoptosis in Respiratory and Limb Muscles of Patients with COPD
Abstract
Discrepancies exist regarding the involvement of cellular inflammation and apoptosis in the muscle dysfunction of chronic obstructive pulmonary disease (COPD) patients with preserved body composition. We explored whether levels of inflammatory cells and apoptosis were increased in both respiratory and limb muscles of COPD patients without nutritional abnormalities. In the vastus lateralis, external intercostals, and diaphragms of severe and moderate COPD patients with normal body composition, and in healthy subjects, intramuscular leukocytes and macrophage levels were determined (immunohistochemistry). Muscle structure was also evaluated. In the diaphragm and vastus lateralis of severe and moderate COPD patients and controls, apoptotic nuclei were explored using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, electron microscopy, and caspase-3 expression. In COPD patients compared with controls, diaphragm and intercostal levels of inflammatory cells were extremely low and not significantly different. However, in the vastus lateralis of the severe patients, inflammatory cell counts, although also very low, were significantly greater. In those patients, TUNEL-positive nuclei levels were also significantly greater in diaphragms and vastus lateralis. A significant inverse relationship was found between quadriceps TUNEL-positive nuclei levels and muscle force. Ultrastructural apoptotic nuclei revealed no differences in respiratory or limb m…
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Two studies failed to report increased caspase-3 expression in COPD patients.
“Noteworthy, these two studies failed to report an increased caspase-3 expression in COPD patients [112,113].”
From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [113]
Only one study has reported increased TUNEL-positive cells in quadriceps and diaphragm muscles of COPD patients, with no ultrastructural difference in apoptotic nuclei.
“only one study reported increased terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL)-positive cells in quadriceps and diaphragm muscles [113]. However, no ultrastructural difference in apoptotic nuclei was noticed.”
From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [113]
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Resistance exercise decreases skeletal muscle tumor necrosis factor α in frail elderly humans
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Skeletal Muscle Apoptosis and Weight Loss in Chronic Obstructive Pulmonary Disease
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Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting
Henrot, P., Dupin, I., Schilfarth, P., Esteves, P., Blervaque, L., Zysman, M., Gouzi, F., Hayot, M., Pomiès, P., & Berger, P. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24076454 · Cited by 85 · see citing works
“Since the early reports of Agusti and colleagues and Gosker and colleagues, who observed, respectively, an increased and unchanged myonuclear fragmentation in muscle cells [111,112], only one study reported increased terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL)-positive cells in quadri…”
BackgroundResultLong-Term PGC1β Overexpression Leads to Apoptosis, Autophagy and Muscle Wasting
Sopariwala, D. H., Yadav, V., Badin, P.-M., Likhite, N., Sheth, M., Lorca, S., Vila, I. K., Kim, E. R., Tong, Q., Song, M. S., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-10238-9 · Cited by 14 · see citing works
- Authors
- Barreiro, E., Ferrer, D., Sanchez, F., Minguella, J., Marin-Corral, J., Martinez-Llorens, J., Lloreta, J., & Gea, J.
- Affiliations
- Universitat Pompeu Fabra (ES)
- Universitat Autònoma de Barcelona (ES)
- Pulmonology Department-Muscle Research and Respiratory System Unit,
- Corresponding author
- Barreiro, Esther
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 2011-09
- Volume
- 111
- Issue
- 3
- Pages
- 808-817
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2082880730
- Cited by
- 79
- References
- 3
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 1 · 2020: 4 · 2019: 4 · 2018: 5 · 2017: 11 · 2016: 10 · 2015: 7 · 2014: 11 · 2013: 10 · 2012: 4
- FWCI
- 3.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Keywords
- chronic obstructive pulmonary disease, muscle inflammation, respiratory muscles, limb muscles, diaphragm, intercostal muscles, vastus lateralis, muscle dysfunction, caspase-3, TUNEL, muscle force, muscle ultrastructure, muscle remodeling
- MeSH terms
- Aged, Analysis of Variance, Biopsy, Humans, Immunohistochemistry, Leukocytes, Macrophages, Male, Microscopy, Electron, Middle Aged, Respiratory Muscles, Severity of Illness Index, Case-Control Studies, Apoptosis, In Situ Nick-End Labeling, Pulmonary Disease, Chronic Obstructive, Lower Extremity, Quadriceps Muscle, Muscle Strength
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