Embryonic Cord Transplants in Peripheral Nerve Restore Skeletal Muscle Function
Abstract
The rapid atrophy of skeletal muscle after denervation severely compromises efforts to restore muscle function. We have transplanted embryonic day 14-15 (E14-E15) ventral spinal cord cells into adult Fischer rat tibial nerve stump to provide neurons for reinnervation. Our aim was to evaluate medial gastrocnemius reinnervation physiologically because this transplant strategy will only be effective if the reinnervated muscle contracts, generates sufficient force to induce joint movement, and is fatigue resistant enough to shorten repeatedly. Twelve weeks posttransplantation, brief duration electrical stimuli applied to the transplants induced medial gastrocnemius contractions that were strong enough to produce ankle movement in 4 of 12 rats (33%). The force of these four "low-threshold" reinnervated muscles and control muscles declined only gradually during five hours of intermittent, supramaximal stimulation and without depression of EMG potential area, which is strong evidence of functional neuromuscular junctions and fatigue resistant muscles. Sectioning of the medial gastrocnemius nerves confirmed that these contractions were innervation dependent. Weakness in low-threshold reinnervated muscles (8% control force) related to incomplete reinnervation, reductions in muscle fiber size, specific tension, and/or the presence of nonfunctional neuromuscular junctions. Muscle reinnervation achieved using this novel transplantation strategy may salvage completely denervated muscle an…
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An animal model for a biohybrid approach to cure flaccid paralysis involves transplanting embryonic spinal cord cells into a container adapted to the distal nerve stump to restore skeletal muscle function.
“researchers have developed an animal model for a biohybrid approach to cure flaccid paralysis that involves embryonic spinal cord cell transplantation into a container adapted to the distal nerve stump to restore skeletal muscle function (Katsuki et al., 1997; Thomas et al., 2000; Klinge et al., 2001b)”
From A critical review of interfaces with the peripheral nervous system for the control of neuroprostheses and hybrid bionic systems (2005) · cites this paper as Thomas et al., 2000
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References 13 · Cited by 60
- Authors
- Thomas, C. K., Erb, D. E., Grumbles, R. M., & Bunge, R. P.
- Affiliations
- University of Miami (US)
- Venue
- Journal of Neurophysiology
- Publisher
- American Physiological Society
- Published
- 2000-07
- Volume
- 84
- Issue
- 1
- Pages
- 591-595
- ISSN
- 0022-3077 (ISSN-L)
- 1522-1598
- OpenAlex
- W2240953638
- Cited by
- 60
- References
- 13
- Citations by year
- 2022: 2 · 2021: 1 · 2018: 2 · 2017: 2 · 2016: 5 · 2015: 3 · 2014: 2 · 2013: 5 · 2012: 6
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurophysiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS-39098)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Genetics › Neurogenetic and Muscular Disorders Research
- Keywords
- denervation-induced muscle atrophy, skeletal muscle function, medial gastrocnemius, neuromuscular junction, muscle fatigue resistance, muscle force production, axon regeneration
- MeSH terms
- Animals, Axons, Female, Movement, Muscle Denervation, Nerve Regeneration, Pregnancy, Rats, Inbred F344, Spinal Cord, Tibial Nerve, Fetal Tissue Transplantation, Muscle, Skeletal, Muscle Fatigue, Muscle Weakness, Recovery of Function, Rats
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