Cited by 88Cardiovascular Function and Risk FactorsCardiac electrophysiology and arrhythmiasCardiac Fibrosis and Remodeling

Influence of genetic background on ex vivo and in vivo cardiac function in several commonly used inbred mouse strains

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Barnabei, Matthew S., Palpant, Nathan J., Metzger, Joseph M.·2010·Physiological Genomics

Abstract

Inbred mouse strains play a critical role in biomedical research. Genetic homogeneity within inbred strains and their general amenability to genetic manipulation have made them an ideal resource for dissecting the physiological function(s) of individual genes. However, the inbreeding that makes inbred mice so useful also results in genetic divergence between them. This genetic divergence is often unaccounted for but may be a confounding factor when comparing studies that have utilized distinct inbred strains. Here, we compared the cardiac function of C57BL/6J mice to seven other commonly used inbred mouse strains: FVB/NJ, DBA/2J, C3H/HeJ, BALB/cJ, 129X1/SvJ, C57BL/10SnJ, and 129S1/SvImJ. The assays used to compare cardiac function were the ex vivo isolated Langendorff heart preparation and in vivo real-time hemodynamic analysis using conductance micromanometry. We report significant strain-dependent differences in cardiac function between C57BL/6J and other commonly used inbred strains. C57BL/6J maintained better cardiac function than most inbred strains after ex vivo ischemia, particularly compared with 129S1/SvImJ, 129X1/SvJ, and C57BL/10SnJ strains. However, during in vivo acute hypoxia 129X1/SvJ and 129S1/SvImJ maintained relatively normal cardiac function, whereas C57BL/6J animals showed dramatic cardiac decompensation. Additionally, C3H/HeJ showed rapid and marked cardiac decompensation in response to esmolol infusion compared with effects of other strains. These findings demonstrate the complex effects of genetic divergence between inbred strains on cardiac function. These results may help inform analysis of gene ablation or transgenic studies and further demonstrate specific quantitative traits that could be useful in discovery of genetic modifiers relevant to cardiac health and disease.

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Crossref · 10.1152/physiolgenomics.00071.2010

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  • Various cardiac functions, parameters, properties, and responses to stress vary depending on mouse strains.

    “various cardiac functions, parameters, properties, and response to stress vary depending on the mouse strains (Barnabei, Palpant, & Metzger, 2010; Hoit, et al., 2002; Howden, et al., 2008; A. P. Shah, et al., 2010; Stull, et al., 2006; van den Borne, et al., 2009).”

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Authors
Barnabei, M. S., Palpant, N. J., & Metzger, J. M.
Affiliations
University of Minnesota (US)
Corresponding author
Metzger, Joseph M.
Venue
Physiological Genomics
Published
2010-10
Volume
42A
Issue
2
Pages
103-113
ISSN
  • 1094-8341 (ISSN-L)
  • 1531-2267
OpenAlex
W2072625807
Cited by
88
References
62
Citations by year
2026: 3 · 2025: 1 · 2024: 2 · 2023: 7 · 2022: 5 · 2021: 4 · 2020: 10 · 2019: 3 · 2018: 7 · 2017: 4 · 2016: 11 · 2015: 11 · 2014: 5 · 2013: 7 · 2012: 5
FWCI
3.29
Type
journal article
Language
en
Open access
Locations
  • Physiological Genomics (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (R01HL059301)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
Keywords
inbred mouse strains, genetic divergence, cardiac function, C57BL/6J mice, C3H/HeJ, DBA/2J, BALB/cJ, ischemia, acute hypoxia, esmolol, acute decompensated heart failure, genetic modifiers, mouse strain comparison
MeSH terms
Adrenergic beta-Antagonists, Animals, Hypoxia, Heart, Heart Function Tests, Hemodynamics, Mice, Inbred Strains, Mice, Transgenic, Myocardial Ischemia, Mice, Embryonic Stem Cells, In Vitro Techniques

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