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Cited by 84Mesenchymal stem cell researchHematopoietic Stem Cell TransplantationBone Tissue Engineering Materials

Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential

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Cicione, Claudia, Muiños-López, Emma, Hermida-Gómez, Tamara, Fuentes-Boquete, Isaac, Díaz-Prado, Silvia, Blanco, Francisco J.·2013·Stem Cells International
DOI 10.1155/2013/232896PMC PMC3777136
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Abstract

Background. The interests in mesenchymal stem cells (MSCs) and their application in cell therapy have resulted in a better understanding of the basic biology of these cells. Recently hypoxia has been indicated as crucial for complete chondrogenesis. We aimed at analyzing bone marrow MSCs (BM-MSCs) differentiation capacity under normoxic and severe hypoxic culture conditions.Methods. MSCs were characterized by flow cytometry and differentiated towards adipocytes, osteoblasts, and chondrocytes under normoxic or severe hypoxic conditions. The differentiations were confirmed comparing each treated point with a control point made of cells grown in DMEM and fetal bovine serum (FBS).Results. BM-MSCs from the donors displayed only few phenotypical differences in surface antigens expressions. Analyzing marker genes expression levels of the treated cells compared to their control point for each lineage showed a good differentiation in normoxic conditions and the absence of this differentiation capacity in severe hypoxic cultures.Conclusions. In our experimental conditions, severe hypoxia affects the in vitro differentiation potential of BM-MSCs. Adipogenic, osteogenic, and chondrogenic differentiations are absent in severe hypoxic conditions. Our work underlines that severe hypoxia slows cell differentiation by means of molecular mechanisms since a decrease in the expression of adipocyte-, osteoblast-, and chondrocyte-specific genes was observed.

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  • In neurological diseases, proteomic analysis revealed that hypoxia enhances the expression of proteins associated with neuroprotection, neural survival, differentiation, and axonal and neurite growth compared to the secretome obtained from normoxia-cultured MSCs.

    “proteomic analysis revealed that hypoxia enhances the expression of proteins [128], [129], [130] associated to neuroprotection, neural survival, differentiation, and axonal and neurite growth, when compared to the secretome obtained from normoxia-cultured MSCs [128].”

    From Mesenchymal stem cell secretome for regenerative medicine: Where do we stand? (2025) · cites this paper as [129]

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Authors
Cicione, C., Muiños-López, E., Hermida-Gómez, T., Fuentes-Boquete, I., Díaz-Prado, S., & Blanco, F. J.
Affiliations
  • Universidade da Coruña (ES)
  • Rheumatology Division, INIBIC Hospital Universitario A Coruña, C/As Xubias S/N, 15006 A Coruña, Spain
  • CIBER-BBN-Cellular Therapy Area, Hospital Universitario A Coruña, C/As Xubias S/N, 15006 A Coruña, Spain
Corresponding author
Blanco, Francisco J.
ORCID
Venue
Stem Cells International
Publisher
Hindawi Limited
Published
2013
Volume
2013
Pages
1-11
ISSN
  • 1687-966X (ISSN-L)
  • 1687-9678
DOI
10.1155/2013/232896
PMC
PMC3777136
OpenAlex
W2071227792
Cited by
84
References
2
Citations by year
2025: 2 · 2024: 3 · 2023: 6 · 2022: 6 · 2021: 7 · 2020: 9 · 2019: 8 · 2018: 7 · 2017: 8 · 2016: 17 · 2015: 5 · 2014: 6
FWCI
4.60
Type
journal article
Language
en
Open access
Licence
Locations
  • Stem Cells International (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Hispana (submittedVersion, cc-by)
  • Repositorio institucional da Universidade de Santiago de Compostela (University of Santiago de Compostela) (submittedVersion, other-oa)
  • RUC (Universidade Da Coruña) (submittedVersion, cc-by)
  • RUNA. Repositorio Institucional del Sergas (Servicio Gallego de Salud (SERGAS)) (submittedVersion, cc-by)
Funders
  • Xunta de Galicia (PS07/84, CB06-01-0040, PLE2009-0144, 08/2028)
  • Universidade da Coruña and Instituto de Salud Carlos (PS07/84, CB06-01-0040, PLE2009-0144, 08/2028)
  • Ministerio Ciencia e Innovacion (PS07/84, CB06-01-0040, PLE2009-0144, 08/2028)
  • Fondo Investigacion Sanitaria-PI (PS07/84, CB06-01-0040, PLE2009-0144, 08/2028)
  • Fondo de Investigación Sanitaria (PS07/84, CB06-01-0040, PLE2009-0144, 08/2028)
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Keywords
bone marrow mesenchymal stem cells, severe hypoxia, stem cell differentiation, adipogenesis, osteogenic differentiation, chondrogenic differentiation, lineage-specific gene expression, normoxia, cell therapy

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