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Cited by 56Heavy Metal Exposure and ToxicitySperm and Testicular FunctionCoenzyme Q10 studies and effects

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Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats

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El-khadragy, Manal, Al-Megrin, Wafa A., AlSadhan, Norah A., Metwally, Dina M., El-Hennamy, Rehab E., Salem, Fatma Elzahraa H., Kassab, Rami B., Abdel Moneim, Ahmed E.·2020·Oxidative Medicine and Cellular Longevity
DOI 10.1155/2020/4981386PMC PMC7290879
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Abstract

Exposure to lead (Pb) causes multiorgan dysfunction including reproductive impairments. Here, we examined the protective effects of coenzyme Q10 (CoQ10) administration on testicular injury induced by lead acetate (PbAc) exposure in rats. This study employed four experimental groups (n=7) that underwent seven days of treatment as follows: control group intraperitoneally (i.p.) treated with 0.1 ml of 0.9% NaCl containing 1% Tween 80 (v:v), CoQ10 group that was i.p. injected with 10 mg/kg CoQ10, PbAc group that was i.p. treated with PbAc (20 mg/kg), and PbAc+CoQ10 group that was i.p. injected with CoQ10 2 h after PbAc. PbAc injection resulted in increasing residual Pb levels in the testis and reducing testosterone, luteinizing hormone, and follicle-stimulating hormone levels. Additionally, PbAc exposure resulted in significant oxidative damage to the tissues on the testes. PbAc raised the levels of prooxidants (malondialdehyde and nitric oxide) and reduced the amount of endogenous antioxidative proteins (glutathione and its derivative enzymes, catalase, and superoxide dismutase) available in the cell. Moreover, PbAc induced the inflammatory response as evidenced by the upregulation of inflammatory mediators (tumor necrosis factor-alpha and interleukin-1 beta). Further, PbAc treatment induced apoptosis in the testicular cells, as indicated by an increase in Bax and caspase 3 expression, and reduced Bcl2 expression. CoQ10 supplementation improved testicular function by inhibiting Pb accumulation, oxidative stress, inflammation, cell death, and histopathological changes following PbAc exposure. Our findings suggest that CoQ10 can act as a natural therapeutic agent to protect against the reproductive impairments associated with PbAc exposure.

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Figure 1 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the concentration of Pb in the testis of rats treated with lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.
Figure 1 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the concentration of Pb in the testis of rats treated with lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.CC BY · PubMed Central
Figure 2 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the absolute and relative testicular weight in rats treated with lead acetate (PbAc, 20 mg/kg). All data represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗Significant variation at p < 0.01 as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.
Figure 2 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the absolute and relative testicular weight in rats treated with lead acetate (PbAc, 20 mg/kg). All data represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗Significant variation at p < 0.01 as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.CC BY · PubMed Central
Figure 3 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the plasma levels of testosterone, LH, and FSH in rats treated with lead acetate (PbAc, 20 mg/kg). All data represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.
Figure 3 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the plasma levels of testosterone, LH, and FSH in rats treated with lead acetate (PbAc, 20 mg/kg). All data represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.CC BY · PubMed Central
Figure 4 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on lipid peroxidation (LPO), nitric oxide (NO), and reduced glutathione (GSH) concentrations in the testis of rats treated with lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.
Figure 4 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on lipid peroxidation (LPO), nitric oxide (NO), and reduced glutathione (GSH) concentrations in the testis of rats treated with lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.CC BY · PubMed Central
Figure 5 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and their corresponding mRNA expressions in the testis of rats exposed to lead acetate (PbAc, 20 mg/kg). Data of the antioxidant enzyme activity was represented as mean ± SEM (n = 7), while the values of mRNA expressions (mean ± SEM of triplicate assays) were normalized to the Actb mRNA levels and are expressed as the fold change relative to the control group. A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.
Figure 5 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and their corresponding mRNA expressions in the testis of rats exposed to lead acetate (PbAc, 20 mg/kg). Data of the antioxidant enzyme activity was represented as mean ± SEM (n = 7), while the values of mRNA expressions (mean ± SEM of triplicate assays) were normalized to the Actb mRNA levels and are expressed as the fold change relative to the control group. A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.CC BY · PubMed Central
Figure 6 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the mRNA levels of Nfe212 and Hmox1 in the testes of rats exposed to lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM of triplicate assays. Data was first normalized to the Actb mRNA levels then was expressed as a fold change relative to the control group. A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively.
Figure 6 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the mRNA levels of Nfe212 and Hmox1 in the testes of rats exposed to lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM of triplicate assays. Data was first normalized to the Actb mRNA levels then was expressed as a fold change relative to the control group. A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively.CC BY · PubMed Central
Figure 7 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the testis of rats treated with lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively.
Figure 7 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the testis of rats treated with lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM (n = 7). A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively.CC BY · PubMed Central
Figure 8 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the mRNA expression of Bcl2, Bax, and Casp3 in the testes of rats exposed to lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM of triplicate assays. Data was first normalized to the Actb mRNA levels then was expressed as the fold change relative to the control group. A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.
Figure 8 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the mRNA expression of Bcl2, Bax, and Casp3 in the testes of rats exposed to lead acetate (PbAc, 20 mg/kg). Data was represented as mean ± SEM of triplicate assays. Data was first normalized to the Actb mRNA levels then was expressed as the fold change relative to the control group. A,BSignificant change at p < 0.05; ∗,∗∗Significant variation at p < 0.01 and p < 0.001, respectively, as compared to the control and PbAc groups, respectively. Data was analyzed by one-way ANOVA using Duncan's post hoc test.CC BY · PubMed Central
Figure 9 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the caspase-3 expression in the testis tissue after lead acetate (PbAc, 20 mg/kg) exposure. (a) Control group, (b) CoQ10-administered group, (c) PbAc-exposed group, and (d) PbAc+CoQ10-treated group. Scale bar = 100 μm.
Figure 9 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the caspase-3 expression in the testis tissue after lead acetate (PbAc, 20 mg/kg) exposure. (a) Control group, (b) CoQ10-administered group, (c) PbAc-exposed group, and (d) PbAc+CoQ10-treated group. Scale bar = 100 μm.CC BY · PubMed Central
Figure 10 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the histopathological changes in the testis tissue after lead acetate (PbAc, 20 mg/kg) exposure. (a) Control group, (b) CoQ10-administered group, (c) PbAc-exposed group, and (d) PbAc+CoQ10-treated group. Scale bar = 100 μm.
Figure 10 Effects of coenzyme Q10 (CoQ10, 10 mg/kg) administration on the histopathological changes in the testis tissue after lead acetate (PbAc, 20 mg/kg) exposure. (a) Control group, (b) CoQ10-administered group, (c) PbAc-exposed group, and (d) PbAc+CoQ10-treated group. Scale bar = 100 μm.CC BY · PubMed Central

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Research works printed but not linked: 4

  1. 2010, African Journal of Biotechnology, 9, 7216
  2. 2005, Bulletin of the Veterinary Institute in Pulawy, 49, 89
  3. 1967, The Journal of Laboratory and Clinical Medicine, 70, 158
  4. 2019, Avicenna journal of phytomedicine

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Metadata

Authors
El-khadragy, M., Al-Megrin, W. A., AlSadhan, N. A., Metwally, D. M., El-Hennamy, R. E., Salem, F. E. H., Kassab, R. B., & Abdel Moneim, A. E.
Affiliations
  • Biology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh 84428, Saudi Arabia
  • Almaarefa University (SA)
  • Zagazig University (EG)
  • Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo 11795, Egypt
  • Taibah University (SA)
  • Al Baha University (SA)
ORCID
Venue
Oxidative Medicine and Cellular Longevity
Publisher
Wiley
Published
2020-06-03
Volume
2020
Pages
1-12
ISSN
  • 1942-0994 (ISSN-L)
  • 1942-0900
DOI
10.1155/2020/4981386
PMC
PMC7290879
OpenAlex
W3033636297
Cited by
56
References
57
Citations by year
2026: 4 · 2025: 9 · 2024: 9 · 2023: 13 · 2022: 12 · 2021: 7 · 2020: 2
FWCI
3.40
Type
journal article
Language
en
Open access
Licence
Locations
  • Oxidative Medicine and Cellular Longevity (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Princess Nourah Bint Abdulrahman University
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Coenzyme Q10 studies and effects
Keywords
coenzyme Q10, lead acetate, lead toxicity, testicular damage, oxidative stress, reproductive toxicity, antioxidant enzymes, inflammation, apoptosis, testosterone, luteinizing hormone, follicle-stimulating hormone, lead accumulation, neuroprotection
MeSH terms
Animals, Follicle Stimulating Hormone, Glutathione, Male, Nitric Oxide, Organometallic Compounds, RNA, Messenger, Testis, Testosterone, Tumor Necrosis Factor-alpha, Ubiquinone, Lipid Peroxidation, Rats, Wistar, bcl-2-Associated X Protein, Caspase 3, Interleukin-1beta

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