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Dicer‐Derived MicroRNAs Are Utilized by the Fragile X Mental Retardation Protein for Assembly on Target RNAs

Plante, Isabelle, Davidovic, Laetitia, Ouellet, Dominique L., Gobeil, Lise-Andrée, Tremblay, Sandra, Khandjian, Edouard W., Provost, Patrick·2006·BioMed Research International
DOI 10.1155/jbb/2006/64347PMC PMC1698263
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Abstract

In mammalian cells, fragile X mental retardation protein (FMRP) has been reported to be part of a microRNA (miRNA)‐containing effector ribonucleoprotien (RNP) complex believed to mediate translational control of specific mRNAs. Here, using recombinant proteins, we demonstrate that human FMRP can act as a miRNA acceptor protein for the ribonuclease Dicer and facilitate the assembly of miRNAs on specific target RNA sequences. The miRNA assembler property of FMRP was abrogated upon deletion of its single‐stranded (ss) RNA binding K‐homology domains. The requirement of FMRP for efficient RNA interference (RNAi) in vivo was unveiled by reporter gene silencing assays using various small RNA inducers, which also supports its involvement in an ss small interfering RNA (siRNA)‐containing RNP (siRNP) effector complex in mammalian cells. Our results define a possible role for FMRP in RNA silencing and may provide further insight into the molecular defects in patients with the fragile X syndrome.

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Authors
Plante, I., Davidovic, L., Ouellet, D. L., Gobeil, L.-A., Tremblay, S., Khandjian, E. W., & Provost, P.
Venue
BioMed Research International
Publisher
Wiley
Published
2006-01
Volume
2006
Issue
1
ISSN
  • 2314-6133
  • 2314-6141
DOI
10.1155/jbb/2006/64347
PMC
PMC1698263
Cited by
52
References
2
Language
en
Licence
creativecommons.org/licenses/by/3.0/ (vor, 248 d)
Funders
  • Canadian Institutes of Health Research (EOP-64706, IGI-44012, NDG-70190)
  • Institute of Neurosciences, Mental Health and Addiction (EOP-64706, IGI-44012, NDG-70190)
  • Institute of Genetics (EOP-64706, IGI-44012, NDG-70190)

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