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Cited by 48Cancer-related molecular mechanisms researchDNA Repair MechanismsCircular RNAs in diseases

Long Noncoding RNA NIHCOLE Promotes Ligation Efficiency of DNA Double-Strand Breaks in Hepatocellular Carcinoma

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Unfried, Juan P., Marín-Baquero, Mikel, Rivera-Calzada, Ángel, Razquin, Nerea, Martín-Cuevas, Eva M., de Bragança, Sara, Aicart-Ramos, Clara, McCoy, Christopher +11 more·2021·Cancer Research
DOI 10.1158/0008-5472.can-21-0463PMC PMC8488005

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Abstract

Long noncoding RNAs (lncRNA) are emerging as key players in cancer as parts of poorly understood molecular mechanisms. Here, we investigated lncRNAs that play a role in hepatocellular carcinoma (HCC) and identified NIHCOLE, a novel lncRNA induced in HCC with oncogenic potential and a role in the ligation efficiency of DNA double-stranded breaks (DSB). NIHCOLE expression was associated with poor prognosis and survival of HCC patients. Depletion of NIHCOLE from HCC cells led to impaired proliferation and increased apoptosis. NIHCOLE deficiency led to accumulation of DNA damage due to a specific decrease in the activity of the nonhomologous end-joining (NHEJ) pathway of DSB repair. DNA damage induction in NIHCOLE-depleted cells further decreased HCC cell growth. NIHCOLE was associated with DSB markers and recruited several molecules of the Ku70/Ku80 heterodimer. Further, NIHCOLE putative structural domains supported stable multimeric complexes formed by several NHEJ factors including Ku70/80, APLF, XRCC4, and DNA ligase IV. NHEJ reconstitution assays showed that NIHCOLE promoted the ligation efficiency of blunt-ended DSBs. Collectively, these data show that NIHCOLE serves as a scaffold and facilitator of NHEJ machinery and confers an advantage to HCC cells, which could be exploited as a targetable vulnerability. Significance: This study characterizes the role of lncRNA NIHCOLE in DNA repair and cellular fitness in HCC, thus implicating it as a therapeutic target. See related commentary by Barcena-Varela and Lujambio, p. 4899

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  • The genes with promoter DNA hypomethylation include AFP, several HCC-related lncRNAs (LINC01419, LINC02163, LINC02835), and tumor-associated antigens such as the MAGE gene family.

    “These genes included AFP, a diagnostic marker for HCC, several HCC-related lncRNAs (LINC01419, LINC02163, and LINC02835), and tumor-associated antigens such as the MAGE gene family35–37.”

    From Multi-omic analysis of hepatocellular carcinoma reveals aberrant cis-regulatory changes and dysregulated retrotransposons with prognostic potential (2025) · cites this paper as [35]

    The Results section identifies LINC02163 as NIHCOLE, an lncRNA highly expressed in HCC. It does not report promoter DNA hypomethylation for LINC02163 or the listed genes, and the passages do not establish the other named genes as members of the claimed group. No page number is available in our copy. (LLM, conf=0.82)

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Research works printed but not linked: 55

  1. [1]Iyer MK, Niknafs YS, Malik R, Singhal U, Sahu A, Hosono Y, The landscape of long noncoding RNAs in the human transcriptome. Nat Genet [Internet]. 2015; 47: 199– 208. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25599403 25599403 PMC4417758
  2. [2]Unfried JP, Sangro P, Prats-Mari L, Sangro B, Fortes P. The Landscape of lncRNAs in Hepatocellular Carcinoma: A Translational Perspective. Cancers (Basel) [Internet]. 2021; 13: 2651. Available from: https://www.mdpi.com/2072-6694/13/11/2651 34071216 PMC8197910
  3. [3]Quinn JJ, Chang HY. Unique features of long non-coding RNA biogenesis and function. Nat Rev Genet [Internet]. Nature Publishing Group; 2016; 17: 47– 62. Available from: http://www.nature.com/articles/nrg.2015.10 26666209
  4. [4]Ulitsky I Evolution to the rescue: Using comparative genomics to understand long non-coding RNAs. Nat Rev Genet [Internet]. Nature Publishing Group; 2016; 17: 601– 14. Available from: 10.1038/nrg.2016.85 27573374 · DOI
  5. [5]Unfried JP, Serrano G, Suarez B, Sangro P, Ferretti V, Prior C, Identification of coding and long noncoding RNAs differentially expressed in tumors and preferentially expressed in healthy tissues. Cancer Res. 2019; 79. 31387921
  6. [6]Hanahan D, Weinberg RA. Hallmarks of cancer: The next generation. Cell. 2011. page 646– 74. 21376230
  7. [7]Tubbs A, Nussenzweig A. Endogenous DNA Damage as a Source of Genomic Instability in Cancer. Cell [Internet]. Cell Press; 2017 [ cited 2018 Mar 20]; 168: 644– 56. Available from: https://www.sciencedirect.com/science/article/pii/S0092867417300053?via%3Dihub 28187286 PMC6591730
  8. [8]Zhao B, Rothenberg E, Ramsden DA, Lieber MR. The molecular basis and disease relevance of non-homologous DNA end joining. Nat Rev Mol Cell Biol. Springer US; 2020; 21: 765– 81. PMC8063501 33077885
  9. [9]Williams GJ, Hammel M, Radhakrishnan SK, Ramsden D, Lees-Miller SP, Tainer JA. Structural insights into NHEJ: Building up an integrated picture of the dynamic DSB repair super complex, one component and interaction at a time. DNA Repair (Amst) [Internet]. 2014; 17: 110– 20. Available from: https://linkinghub.elsevier.com/retrieve/pii/S1568786414000469 24656613 PMC4102006
  10. [10]Chaplin AK, Hardwick SW, Liang S, Kefala Stavridi A, Hnizda A, Cooper LR, Dimers of DNA-PK create a stage for DNA double-strand break repair. Nat Struct Mol Biol. 2020; 33077952
  11. [11]Chen S, Lee L, Naila T, Fishbain S, Wang A, Tomkinson AE, Structural basis of long-range to short-range synaptic transition in NHEJ. Nature [Internet]. 2021; 593: 294– 8. Available from: http://www.nature.com/articles/s41586-021-03458-7 33854234 PMC8122075
  12. [12]Hammel M, Yu Y, Radhakrishnan SK, Chokshi C, Tsai MS, Matsumoto Y, An intrinsically disordered APLF links Ku, DNA-PKcs, and XRCC4-DNA ligase IV in an extended flexible non-homologous end joining complex. J Biol Chem. 2016; 291: 26987– 7006. 27875301 PMC5207133
  13. [13]Grundy GJ, Rulten SL, Zeng Z, Arribas-Bosacoma R, Iles N, Manley K, APLF promotes the assembly and activity of non-homologous end joining protein complexes. EMBO J [Internet]. Nature Publishing Group; 2013; 32: 112– 25. Available from: 10.1038/emboj.2012.304 23178593 PMC3545299 · DOI
  14. [14]Brouwer I, Sitters G, Candelli A, Heerema SJ, Heller I, Melo De AJ, Sliding sleeves of XRCC4-XLF bridge DNA and connect fragments of broken DNA. Nature. 2016; 535: 566– 9. 27437582
  15. [15]Chang HHY, Watanabe G, Gerodimos CA, Ochi T, Blundell TL, Jackson SP, Different DNA end configurations dictate which NHEJ components are most important for joining efficiency. J Biol Chem. 2016; 291: 24377– 89. 27703001 PMC5114395
  16. [16]Xing M, Yang M, Huo W, Feng F, Wei L, Jiang W, Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. Nat Commun. 2015; 6. PMC4339890 25670504
  17. [17]Thapar R, Wang JL, Hammel M, Ye R, Liang K, Sun C, Mechanism of efficient double-strand break repair by a long non-coding RNA. Nucleic Acids Res. 2020; 48: 10953– 72. 33045735 PMC7641761
  18. [18]Haemmig S, Yang D, Sun X, Das D, Ghaffari S, Molinaro R, Long noncoding RNA SNHG12 integrates a DNA-PK–mediated DNA damage response and vascular senescence. Sci Transl Med. 2020; 32075942
  19. [19]Wang D, Zhou Z, Wu E, Ouyang C, Wei G, Wang Y, LRIK interacts with the Ku70–Ku80 heterodimer enhancing the efficiency of NHEJ repair. Cell Death Differ. 2020; PMC7852670 32587379
  20. [20]Hirsch TZ, Negulescu A, Gupta B, Caruso S, Noblet B, Couchy G, BAP1 mutations define a homogeneous subgroup of hepatocellular carcinoma with fibrolamellar-like features and activated PKA. J Hepatol. 2020; 72: 924– 36. 31862487
  21. [21]Zhang Y, Feng Y, Hu Z, Hu X, Yuan C-X, Fan Y, Characterization of Long Noncoding RNA-Associated Proteins by RNA-Immunoprecipitation. Genomic Elem Heal Dis Evol [Internet]. New York, NY: Springer New York; 2016. page 19– 26. Available from: http://link.springer.com/10.1007/978-1-4939-3070-8_4 PMC4773202 26721480 · DOI
  22. [22]Marín-Béjar O, Huarte M. RNA Pulldown Protocol for In Vitro Detection and Identification of RNA-Associated Proteins. In: Carmichael GG, editor. New York, NY: Springer New York; 2015. page 87– 95. Available from: http://link.springer.com/10.1007/978-1-4939-1369-5 25240889 · DOI
  23. [23]Moore S, Berger ND, Luijsterburg MS, Piett CG, Stanley FKT, Schräder CU, The CHD6 chromatin remodeler is an oxidative DNA damage response factor. Nat Commun [Internet]. Springer US; 2019; 10. Available from: 10.1038/s41467-018-08111-y PMC6335469 30651562 · DOI
  24. [24]Gilmore JL, Yoshida A, Takahashi H, Deguchi K, Kobori T, Louvet E, Analyses of nuclear proteins and nucleic acid structures using atomic force microscopy. In: Nakagawa S, Hirose T, editors. Methods Mol Biol. New York, NY: Springer New York; 2015. page 119– 53. 25555579
  25. [25]Horcas I, Fernández R, Gómez-Rodríguez JM, Colchero J, Gómez-Herrero J, Baro AM. WSXM: A software for scanning probe microscopy and a tool for nanotechnology. Rev Sci Instrum. 2007; 78: 13705. 17503926
  26. [26]Radhakrishnan SK, Lees-Miller SP. DNA requirements for interaction of the C-terminal region of Ku80 with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs). DNA Repair (Amst) [Internet]. 2017; 57: 17– 28. Available from: https://linkinghub.elsevier.com/retrieve/pii/S1568786416304347 28641126 PMC5568904
  27. [27]Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Bioconductor: open software development for computational biology and bioinformatics. Genome Biol [Internet]. 2004; 5: R80. Available from: http://www.ncbi.nlm.nih.gov/pubmed/15461798%5Cn http://www.ncbi.nlm.nih.gov/sites/entrez 15461798 PMC545600
  28. [28]Volders P-J, Anckaert J, Verheggen K, Nuytens J, Martens L, Mestdagh P, LNCipedia 5: towards a reference set of human long non-coding RNAs. Nucleic Acids Res [Internet]. 2019; 47: D135– 9. Available from: https://academic.oup.com/nar/article/47/D1/D135/5146198 30371849 PMC6323963
  29. [29]Dong L, Hong H, Chen X, Huang Z, Wu W, Wu F. LINC02163 regulates growth and epithelial-to-mesenchymal transition phenotype via miR-593–3p/FOXK1 axis in gastric cancer cells. Artif Cells, Nanomedicine Biotechnol. 2018; 46: 607– 15. 29893595
  30. [30]Ma J, Zhang L, Shang A, Song H, Huo J, Zhang M, LINC02163 promotes colorectal cancer progression via miR-511–3p/AKT3 axis. Artif Cells, Nanomedicine Biotechnol. 2020; 48: 961– 8. 32524841
  31. [31]Qin C, Jin L, Li J, Zha W, Ding H, Liu X, Long non-coding RNA LINC02163 accelerates malignant tumor behaviors in breast cancer by regulating the microRNA-511–3p/HMGA2 axis. Oncol Res Featur Preclin Clin Cancer Ther. 2020; PMC7751230 32571448
  32. [32]Unfried JP, Serrano G, Suárez B, Sangro P, Ferretti V, Prior C, Identification of Coding and Long Noncoding RNAs Differentially Expressed in Tumors and Preferentially Expressed in Healthy Tissues. Cancer Res [Internet]. 2019; 79: 5167– 80. Available from: http://cancerres.aacrjournals.org/lookup/doi/10.1158/0008-5472.CAN-19-0400 31387921 · DOI
  33. [33]Chiang DY, Villanueva A, Hoshida Y, Peix J, Newell P, Minguez B, Focal gains of VEGFA and molecular classification of hepatocellular carcinoma. Cancer Res. 2008; 68: 6779– 88. 18701503 PMC2587454
  34. [34]Cancer Genome Atlas Research Network. Electronic address: wheeler@bcm.edu A, Cancer Genome Atlas Research Network M, Carlsen R, Chuah E, Clarke A, Dhalla N, Comprehensive and Integrative Genomic Characterization of Hepatocellular Carcinoma. Cell [Internet]. Elsevier; 2017 [ cited 2018 Jul 2]; 169: 1327– 1341.e23. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28622513 28622513 PMC5680778
  35. [35]Calderaro J, Couchy G, Imbeaud S, Amaddeo G, Letouzé E, Blanc J-FF, Histological subtypes of hepatocellular carcinoma are related to gene mutations and molecular tumour classification. J Hepatol [Internet]. European Association for the Study of the Liver; 2017 [ cited 2019 Feb 13]; 67: 727– 38. Available from: 10.1016/j.jhep.2017.05.014 28532995 · DOI
  36. [36]Boyault S, Rickman DS, De Reyniès A, Balabaud C, Rebouissou S, Jeannot E, Transcriptome classification of HCC is related to gene alterations and to new therapeutic targets. Hepatology. 2007; 45: 42– 52. 17187432
  37. [37]Nault JC, De Reyniès A, Villanueva A, Calderaro J, Rebouissou S, Couchy G, A hepatocellular carcinoma 5-gene score associated with survival of patients after liver resection. Gastroenterology. 2013; 145: 176– 87. 23567350
  38. [38]Caruso S, Calatayud AL, Pilet J, La Bella T, Rekik S, Imbeaud S, Analysis of Liver Cancer Cell Lines Identifies Agents With Likely Efficacy Against Hepatocellular Carcinoma and Markers of Response. Gastroenterology [Internet]. Elsevier, Inc; 2019; 157: 760– 76. Available from: 10.1053/j.gastro.2019.05.001 31063779 · DOI
  39. [39]Bennardo N, Cheng A, Huang N, Stark JM. Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. Haber JE, editor. PLoS Genet. 2008; 4: e1000110. 18584027 PMC2430616
  40. [40]Bhargava R, Sandhu M, Muk S, Lee G, Vaidehi N, Stark JM. C-NHEJ without indels is robust and requires synergistic function of distinct XLF domains. Nat Commun [Internet]. Springer US; 2018; 9. Available from: 10.1038/s41467-018-04867-5 PMC6021437 29950655 · DOI
  41. [41]Gruber AR, Lorenz R, Bernhart SH, Neuböck R, Hofacker IL. The Vienna RNA websuite. Nucleic Acids Res. 2008; 36: 70– 4. PMC2447809 18424795
  42. [42]Zuker M Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res [Internet]. 2003; 31: 3406– 15. Available from: https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkg595 12824337 PMC169194 · DOI · Paper page
  43. [43]Arosio D, Cui S, Ortega C, Chovanec M, Di Marco S, Baldini G, Studies on the Mode of Ku Interaction with DNA. J Biol Chem [Internet]. 2002; 277: 9741– 8. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0021925819360946 11796732
  44. [44]Fell VL, Schild-Poulter C. The Ku heterodimer: Function in DNA repair and beyond. Mutat Res Mutat Res [Internet]. Elsevier B.V.; 2015; 763: 15– 29. Available from: 10.1016/j.mrrev.2014.06.002 25795113 · DOI
  45. [45]Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR. RNA recognition by the DNA end-binding Ku heterodimer. Rna. 2013; 19: 841– 51. 23610127 PMC3683917
  46. [47]Zhao B, Watanabe G, Morten MJ, Reid DA, Rothenberg E, Lieber MR. The essential elements for the noncovalent association of two DNA ends during NHEJ synapsis. Nat Commun [Internet]. Springer US; 2019; 10: 3588. Available from: http://www.nature.com/articles/s41467-019-11507-z 31399561 PMC6688983
  47. [48]Jackson SP, Bartek J. The DNA-damage response in human biology and disease. Nature. 2009. page 1071– 8. PMC2906700 19847258
  48. [49]Sishc BJ, Davis AJ. The role of the core non-homologous end joining factors in carcinogenesis and cancer. Cancers (Basel). 2017; 9: 81. PMC5532617 28684677
  49. [50]Cary RB, Peterson SR, Wang J, Bear DG, Bradbury EM, Chen DJ. DNA looping by Ku and the DNA-dependent protein kinase. Proc Natl Acad Sci U S A. 1997; 94: 4267– 72. 9113978 PMC20711
  50. [51]Corbeski I, Dolinar K, Wienk H, Boelens R, Van Ingen H. DNA repair factor APLF acts as a H2A-H2B histone chaperone through binding its DNA interaction surface. Nucleic Acids Res. Oxford University Press; 2018; 46: 7138– 52. PMC6101569 29905837
  51. [52]Alberti S, Gladfelter A, Mittag T. Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates. Cell [Internet]. Elsevier Inc.; 2019; 176: 419– 34. Available from: 10.1016/j.cell.2018.12.035 30682370 PMC6445271 · DOI · Paper page
  52. [53]Ting NSY, Yu Y, Pohorelic B, Lees-Miller SP, Beattie TL. Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase. Nucleic Acids Res. 2005; 33: 2090– 8. 15824061 PMC1075923
  53. [54]Zhang Y, He Q, Hu Z, Feng Y, Fan L, Tang Z, Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer. Nat Struct Mol Biol [Internet]. Nature Publishing Group; 2016; 23: 522– 30. Available from: http://www.nature.com/doifinder/10.1038/nsmb.3211 27111890 PMC4927085 · DOI
  54. [55]Peterson SE, Stellwagen AE, Diede SJ, Singer MS, Haimberger ZW, Johnson CO, The function of a stem-loop in telomerase RNA is linked to the DNA repair protein Ku. Nat Genet. 2001; 27: 64– 7. 11138000
  55. [56]Shadrina O, Garanina I, Korolev S, Zatsepin T, Van Assche J, Daouad F, Analysis of RNA binding properties of human Ku protein reveals its interactions with 7SK snRNA and protein components of 7SK snRNP complex. Biochimie. Elsevier Ltd; 2020; 171–172: 110– 23. 32105815

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Authors
Unfried, J. P., Marín-Baquero, M., Rivera-Calzada, Á., Razquin, N., Martín-Cuevas, E. M., de Bragança, S., Aicart-Ramos, C., McCoy, C., Prats-Mari, L., Arribas-Bosacoma, R., Lee, L., Caruso, S., Zucman-Rossi, J., Sangro, B., Williams, G., Moreno-Herrero, F., Llorca, O., Lees-Miller, S. P., & Fortes, P.
Affiliations
  • Centro de Investigación Médica Aplicada (ES)
  • Consejo Superior de Investigaciones Científicas (ES)
  • Spanish National Cancer Research Centre (ES)
  • 2Department of Macromolecular Structures, Spanish National Centre for Biotechnology (CNB), Spanish National Research Council (CSIC), Madrid, Spain.
  • University of Calgary (CA)
  • University of Sussex (GB)
  • Inserm (FR)
  • 6Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, INSERM, Functional Genomics of Solid Tumors laboratory, Équipe Labellisée Ligue Nationale Contre le Cancer, Labex OncoImmunology, Paris, France.
  • Navarre Institute of Health Research (ES)
  • 3Structural Biology Program, Spanish National Cancer Research Center (CNIO), Madrid, Spain.
  • 4Department of Biochemistry and Molecular Biology, Robson DNA Science Centre, Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, Calgary, Canada.
Corresponding author
  • Unfried, Juan P.
  • Fortes, Puri
ORCID
Venue
Cancer Research
Publisher
American Association for Cancer Research (AACR)
Published
2021-07-28
Volume
81
Issue
19
Pages
4910-4925
ISSN
  • 0008-5472 (ISSN-L)
  • 1538-7445
DOI
10.1158/0008-5472.can-21-0463
PMC
PMC8488005
OpenAlex
W3183730523
Cited by
48
References
0
Citations by year
2026: 2 · 2025: 5 · 2024: 13 · 2023: 12 · 2022: 11 · 2021: 5
FWCI
4.18
Type
journal article
Language
en
Open access
Locations
  • Cancer Research (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
Funders
  • Ministry of Economy and Competitiveness (SAF2015-70971-R, BFU2017-83794-P)
  • MCIU/AEI/FEDER/UE (RTI2018-101759-B-I00)
  • NIH (P01CA092584)
  • NSERC (RGPIN-2018-04327)
  • CFI (RCP-18-023-SEG)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Circular RNAs in diseases
Keywords
long non-coding RNA, hepatocellular carcinoma, DNA double-strand break repair, non-homologous end joining, Ku70/Ku80, DNA ligase IV, DNA damage response, cell proliferation, apoptosis, prognostic factors, therapeutic target
MeSH terms
Carcinoma, Hepatocellular, Humans, Liver Neoplasms, Models, Biological, Nucleic Acid Conformation, Prognosis, Biomarkers, Tumor, Gene Expression Regulation, Neoplastic, Gene Expression Profiling, Cell Line, Tumor, DNA Breaks, Double-Stranded, High-Throughput Nucleotide Sequencing, Nucleotide Motifs, DNA End-Joining Repair, RNA, Long Noncoding

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