The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases
Published version of a preprint
Abstract
PURPOSE: The purpose of this study was to determine the accuracy, precision, and linearity of the CellSearch system and evaluate the number of circulating tumor cells (CTCs) per 7.5 mL of blood in healthy subjects, patients with nonmalignant diseases, and patients with a variety of metastatic carcinomas. EXPERIMENTAL DESIGN: The CellSearch system was used to enumerate CTCs in 7.5 mL of blood. Blood samples spiked with cells from tumor cell lines were used to establish analytical accuracy, reproducibility, and linearity. Prevalence of CTCs was determined in blood from 199 patients with nonmalignant diseases, 964 patients with metastatic carcinomas, and 145 healthy donors. RESULTS: Enumeration of spiked tumor cells was linear over the range of 5 to 1,142 cells, with an average recovery of >/=85% at each spike level. Only 1 of the 344 (0.3%) healthy and nonmalignant disease subjects had >/=2 CTCs per 7.5 mL of blood. In 2,183 blood samples from 964 metastatic carcinoma patients, CTCs ranged from 0 to 23,618 CTCs per 7.5 mL (mean, 60 +/- 693 CTCs per 7.5 mL), and 36% (781 of 2,183) of the specimens had >/=2 CTCs. Detection of >/=2 CTCs occurred at the following rates: 57% (107 of 188) of prostate cancers, 37% (489 of 1,316) of breast cancers, 37% (20 of 53) of ovarian cancers, 30% (99 of 333) of colorectal cancers, 20% (34 of 168) of lung cancers, and 26% (32 of 125) of other cancers. CONCLUSIONS: The CellSearch system can be standardized across multiple laboratories and may be used to determine the clinical utility of CTCs. CTCs are extremely rare in healthy subjects and patients with nonmalignant diseases but present in various metastatic carcinomas with a wide range of frequencies.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“While circulating tumor cells (CTCs) are considered a form of liquid biopsy, and we (31) and others (47) have reported on approaches for molecular analysis of CTCs, NSCLC patient CTCs cannot always be reliably identified (44, 48, 49).”
Citations
Cites
National Committee for Clinical Laboratory Standards
Bruck, E. (1980) · PEDIATRICS · doi:10.1542/peds.65.1.187 · Cited by 14,736
Addelman, S., Box, G. E. P., Hunter, W. G., & Hunter, J. S. (1979) · Technometrics · doi:10.2307/1267766 · Cited by 6,753
Circulating Tumor Cells, Disease Progression, and Survival in Metastatic Breast Cancer
Cristofanilli, M., Budd, G. T., Ellis, M. J., Stopeck, A., Matera, J., Miller, M. C., Reuben, J. M., Doyle, G. V., Allard, W. J., Terstappen, L. W. M. M., et al. (2004) · New England Journal of Medicine · doi:10.1056/nejmoa040766 · Cited by 4,602
Detection and characterization of carcinoma cells in the blood
Racila, E. V., Euhus, D., Weiss, A. J., Rao, C., McConnell, J. D., Terstappen, L. W. M. M., & Uhr, J. W. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.8.4589 · Cited by 713
Ross, A., Cooper, B., Lazarus, H. M., Mackay, W., Moss, T., Ciobanu, N., Tallman, M. S., Kennedy, M., Davidson, N., & Sweet, D. (1993) · Blood · doi:10.1182/blood.v82.9.2605.bloodjournal8292605 · Cited by 487
Brugger, W., Bross, K., Glatt, M., Weber, F., Mertelsmann, R. H., & Kanz, L. (1994) · Blood · doi:10.1182/blood.v83.3.636.636 · Cited by 440
Brugger, W., Bross, K., Glatt, M., et al. (1994) · Blood · doi:10.1182/blood.v83.3.636.bloodjournal833636 · Cited by 357
Momburg, F., Moldenhauer, G., Hämmerling, G. J., & Möller, P. (1987) · PubMed · Cited by 328
Kugaya, A., Akechi, T., Okuyama, T., Nakano, T., Mikami, I., Okamura, H., & Uchitomi, Y. (2000) · Cancer · doi:10.1002/1097-0142(20000615)88:12 · Cited by 309
Borgen, E., Naume, B., Nesland, J. M., Kvalheim, G., Beiske, K., Fodstad, Ø., Diel, I., Solomayer, E., Theocharous, P., Coombes, R. C., et al. (1999) · Cytotherapy · doi:10.1080/0032472031000141283 · Cited by 263
Research works printed but not linked: 8
- Soria JC, Gauthier LR, Raymond E, et al Molecular detection of telomerase positive circulating epithelial cells in metastatic breast cancer patients. Clin Cancer Res 1999;5:971-5.
- Fehm T, Sagalowsky A, Clifford E, et al Cytogenetic evidence that circulating epithelial cells in patients with carcinoma are malignant. Clin Cancer Res 2002;8:2073-84.
- Kagan M, Howard D, Bendele T, et al A sample preparation and analysis system for identification of circulating tumor cells. J Clin Ligand Assay 2002;25:104-10.
- Rao C, Rutner H, Hermann M, Russell T, Terstappen LWMM. Preservation of blood specimens to prevent breakdown of circulating tumor cells. Proc Am Assoc Cancer Res 2002;43:734
- Allard WJ, Hayes DF, Repollet MI, et al A cellular preservative improves the specificity and yield of circulating tumor cells in carcinoma patients[abstract A3482]. Proc Am Soc Clin Oncol 2003;22:866
- Motulsky H. The confidence interval of counted variables. Intuitive biostatistics 1995p. 245-9. Oxford University Press New York
- Miller MC, Gross S, Allard WJ, et al Circulating tumor cells (CTCs) predict survival in patients with metastatic prostate cancer[abstract A5270]. Proc Am Assoc Cancer Res 2004;45:1215
- Cristofanilli M, Reuben JM, Fritsche HA, et al Circulating tumor cells (CTCs): a novel prognostic factor for newly diagnosed metastatic breast cancer (MBC)[abstract A1349]. Proc Am Assoc Cancer Res 2003;44:1349
Cited by
Sorted by citations
Circulating Tumor Cells, Disease Progression, and Survival in Metastatic Breast Cancer
Cristofanilli, M., Budd, G. T., Ellis, M. J., Stopeck, A., Matera, J., Miller, M. C., Reuben, J. M., Doyle, G. V., Allard, W. J., Terstappen, L. W. M. M., et al. (2004) · New England Journal of Medicine · doi:10.1056/nejmoa040766 · Cited by 4,602
Isolation of rare circulating tumour cells in cancer patients by microchip technology
Nagrath, S., Sequist, L. V. D., Maheswaran, S., Bell, D. W., Irimia, D., Ulkus, L., Smith, M. R., Kwak, E. L., Digumarthy, S. R., Muzikansky, A., et al. (2007) · Nature · doi:10.1038/nature06385 · Cited by 3,595
Bono, J. S. D., Scher, H. I., Montgomery, B., Parker, C. C., Miller, M. C., Tissing, H., Doyle, G. V., Terstappen, L. W. M. M., Pienta, K. J., & Raghavan, D. (2008) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-08-0872 · Cited by 2,189
Integrating liquid biopsies into the management of cancer
Siravegna, G., Marsoni, S., Siena, S., & Bardelli, A. (2017) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2017.14 · Cited by 1,985
Cohen, S. J., Punt, C. J. A., Iannotti, N. O., Saidman, B. H., Sabbath, K. D., Gabrail, N. Y., Picus, J., Morse, M., Mitchell, E. P., Miller, M. C., et al. (2008) · Journal of Clinical Oncology · doi:10.1200/jco.2007.15.8923 · Cited by 1,876
Metadata
- Authors
- Allard, W. J., Matera, J., Miller, M. C., Repollet, M., Connelly, M. C., Rao, C., Tibbe, A. G. J., Uhr, J. W., & Terstappen, L. W. M. M.
- Affiliations
- 1Immunicon Corporation, Huntingdon Valley, Pennsylvania; and
- The University of Texas Southwestern Medical Center (US)
- Venue
- Clinical Cancer Research
- Published
- 2004-10-15
- Volume
- 10
- Issue
- 20
- Pages
- 6897-6904
- ISSN
- 1078-0432 (ISSN-L)
- 1557-3265
- OpenAlex
- W2116483002
- Cited by
- 2,542
- References
- 30
- Citations by year
- 2026: 66 · 2025: 81 · 2024: 87 · 2023: 135 · 2022: 117 · 2021: 158 · 2020: 169 · 2019: 166 · 2018: 171 · 2017: 193 · 2016: 197 · 2015: 165 · 2014: 177 · 2013: 161 · 2012: 132
- FWCI
- 7.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Cancer Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Keywords
- circulating tumor cells, CellSearch, metastatic carcinoma, prostate cancer, breast cancer, ovarian cancer, colorectal cancer, lung cancer, liquid biopsy
- MeSH terms
- Adult, Automation, Biological Assay, Carcinoma, Cytological Techniques, Female, Humans, Male, Middle Aged, Neoplastic Cells, Circulating, Neoplasm Metastasis, Sensitivity and Specificity, Reproducibility of Results, Case-Control Studies
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
