Cited by 2,542Cancer Genomics and DiagnosticsCancer Cells and MetastasisMicrofluidic and Bio-sensing Technologies

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Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases

Allard, W. Jeffrey, Matera, Jeri, Miller, M. Craig, Repollet, Madeline, Connelly, Mark C., Rao, Chandra, Tibbe, Arjan G. J., Uhr, Jonathan W., Terstappen, Leon W. M. M.·2004·Clinical Cancer Research

DOI 10.1158/1078-0432.ccr-04-0378

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Abstract

PURPOSE: The purpose of this study was to determine the accuracy, precision, and linearity of the CellSearch system and evaluate the number of circulating tumor cells (CTCs) per 7.5 mL of blood in healthy subjects, patients with nonmalignant diseases, and patients with a variety of metastatic carcinomas. EXPERIMENTAL DESIGN: The CellSearch system was used to enumerate CTCs in 7.5 mL of blood. Blood samples spiked with cells from tumor cell lines were used to establish analytical accuracy, reproducibility, and linearity. Prevalence of CTCs was determined in blood from 199 patients with nonmalignant diseases, 964 patients with metastatic carcinomas, and 145 healthy donors. RESULTS: Enumeration of spiked tumor cells was linear over the range of 5 to 1,142 cells, with an average recovery of >/=85% at each spike level. Only 1 of the 344 (0.3%) healthy and nonmalignant disease subjects had >/=2 CTCs per 7.5 mL of blood. In 2,183 blood samples from 964 metastatic carcinoma patients, CTCs ranged from 0 to 23,618 CTCs per 7.5 mL (mean, 60 +/- 693 CTCs per 7.5 mL), and 36% (781 of 2,183) of the specimens had >/=2 CTCs. Detection of >/=2 CTCs occurred at the following rates: 57% (107 of 188) of prostate cancers, 37% (489 of 1,316) of breast cancers, 37% (20 of 53) of ovarian cancers, 30% (99 of 333) of colorectal cancers, 20% (34 of 168) of lung cancers, and 26% (32 of 125) of other cancers. CONCLUSIONS: The CellSearch system can be standardized across multiple laboratories and may be used to determine the clinical utility of CTCs. CTCs are extremely rare in healthy subjects and patients with nonmalignant diseases but present in various metastatic carcinomas with a wide range of frequencies.

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Self-citation
Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering
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Significant and Sustained Antitumor Activity in Post-Docetaxel, Castration-Resistant Prostate Cancer With the CYP17 Inhibitor Abiraterone Acetate
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Self-citation
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ApoStream™, a new dielectrophoretic device for antibody independent isolation and recovery of viable cancer cells from blood
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Targeting the PI3K/AKT Pathway for the Treatment of Prostate Cancer
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Circulating Tumor Cell Detection Predicts Early Metastatic Relapse After Neoadjuvant Chemotherapy in Large Operable and Locally Advanced Breast Cancer in a Phase II Randomized Trial
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Detection of circulating tumour cells with a hybrid (epithelial/mesenchymal) phenotype in patients with metastatic non-small cell lung cancer
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“While circulating tumor cells (CTC) are considered a form of liquid biopsy, and we (31) and others (47) have reported on the approaches for molecular analysis of CTCs, NSCLC patient CTCs cannot always be reliably identified (44, 48, 49).”
Background
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Authors: Allard, W. J., Matera, J., Miller, M. C., Repollet, M., Connelly, M. C., Rao, C., Tibbe, A. G. J., Uhr, J. W., & Terstappen, L. W. M. M.
Affiliations: 1Immunicon Corporation, Huntingdon Valley, Pennsylvania; and
The University of Texas Southwestern Medical Center (US)
ORCID: Miller, M. Craig
Terstappen, Leon W. M. M.
Venue: Clinical Cancer Research
Publisher: American Association for Cancer Research (AACR)
Published: 2004-10-15
Volume: 10
Issue: 20
Pages: 6897-6904
ISSN: 1078-0432 (ISSN-L)
1557-3265
DOI: 10.1158/1078-0432.ccr-04-0378
OpenAlex: W2116483002
Cited by: 2,542
References: 30
Citations by year: 2026: 66 · 2025: 81 · 2024: 87 · 2023: 135 · 2022: 117 · 2021: 158 · 2020: 169 · 2019: 166 · 2018: 171 · 2017: 193 · 2016: 197 · 2015: 165 · 2014: 177 · 2013: 161 · 2012: 132
FWCI: 7.52
Type: journal article
Language: en
Open access: closed
Locations: Clinical Cancer Research (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
Keywords: circulating tumor cells, CellSearch, metastatic carcinoma, prostate cancer, breast cancer, ovarian cancer, colorectal cancer, lung cancer, liquid biopsy
MeSH terms: Adult, Automation, Biological Assay, Carcinoma, Cytological Techniques, Female, Humans, Male, Middle Aged, Neoplastic Cells, Circulating, Neoplasm Metastasis, Sensitivity and Specificity, Reproducibility of Results, Case-Control Studies

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Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases

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