Cited by 332Gut microbiota and healthColorectal Cancer Screening and DetectionBacterial Identification and Susceptibility Testing

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Fecal Bacteria Act as Novel Biomarkers for Noninvasive Diagnosis of Colorectal Cancer

Liang, Qiaoyi, Chiu, Jonathan, Chen, Yingxuan, Huang, Yanqin, Higashimori, Akira, Fang, Jingyuan, Brim, Hassan, Ashktorab, Hassan, Ng, Siew Chien, Ng, Simon Siu Man, Zheng, Shu, Chan, Francis Ka Leung, Sung, Joseph Jao Yiu, Yu, Jun·2017·Clinical Cancer Research

DOI 10.1158/1078-0432.ccr-16-1599

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Abstract

Abstract Purpose: Gut microbiota have been implicated in the development of colorectal cancer. We evaluated the utility of fecal bacterial marker candidates identified by our metagenome sequencing analysis for colorectal cancer diagnosis. Experimental Design: Subjects (total 439; 203 colorectal cancer and 236 healthy subjects) from two independent Asian cohorts were included. Probe-based duplex quantitative PCR (qPCR) assays were established for the quantification of bacterial marker candidates. Results: Candidates identified by metagenome sequencing, including Fusobacterium nucleatum (Fn), Bacteroides clarus (Bc), Roseburia intestinalis (Ri), Clostridium hathewayi (Ch), and one undefined species (labeled as m7), were examined in fecal samples of 203 colorectal cancer patients and 236 healthy controls by duplex-qPCR. Strong positive correlations were demonstrated between the quantification of each candidate by our qPCR assays and metagenomics approach (r = 0.801–0.934, all P < 0.0001). Fn was significantly more abundant in colorectal cancer than controls (P < 0.0001), with AUROC of 0.868 (P < 0.0001). At the best cut-off value maximizing sum of sensitivity and specificity, Fn discriminated colorectal cancer from controls with a sensitivity of 77.7%, and specificity of 79.5% in cohort I. A simple linear combination of four bacteria (Fn + Ch + m7-Bc) showed an improved diagnostic ability compared with Fn alone (AUROC = 0.886, P < 0.0001) in cohort I. These findings were further confirmed in an independent cohort II. In particular, improved diagnostic performances of Fn alone (sensitivity 92.8%, specificity 79.8%) and four bacteria (sensitivity 92.8%, specificity 81.5%) were achieved in combination with fecal immunochemical testing for the detection of colorectal cancer. Conclusions: Stool-based colorectal cancer–associated bacteria can serve as novel noninvasive diagnostic biomarkers for colorectal cancer. Clin Cancer Res; 23(8); 2061–70. ©2016 AACR.

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Comparing the Areas under Two or More Correlated Receiver Operating Characteristic Curves: A Nonparametric Approach
DeLong, E. R., DeLong, D. M., & Clarke-Pearson, D. L. (1988) · Biometrics · doi:10.2307/2531595 · Cited by 22,649
Enterotypes of the human gut microbiome
MetaHIT Consortium (additional members), Arumugam, M., Raes, J., Pelletier, E., Le Paslier, D., Yamada, T., Mende, D. R., Fernandes, G. R., Tap, J., Bruls, T., et al. (2011) · Nature · doi:10.1038/nature09944 · Cited by 7,548
A metagenome-wide association study of gut microbiota in type 2 diabetes
Qin, J., Li, Y., Cai, Z., Li, S., Zhu, J., Zhang, F., Liang, S., Zhang, W., Guan, Y., Shen, D., et al. (2012) · Nature · doi:10.1038/nature11450 · Cited by 6,762
Self-citation
Linking long-term dietary patterns with gut microbial enterotypes
Wu, G. D., Chen, J., Hoffmann, C., Bittinger, K., Chen, Y.-Y., Keilbaugh, S. A., Bewtra, M., Knights, D., Walters, W. A., Knight, R., et al. (2011) · Science · doi:10.1126/science.1208344 · Cited by 6,513
Human Genetics Shape the Gut Microbiome
Goodrich, J. K., Waters, J. L., Poole, A. C., Sutter, J. L., Koren, O., Blekhman, R., Beaumont, M., Van Treuren, W., Knight, R., Bell, J. T., et al. (2014) · Cell · doi:10.1016/j.cell.2014.09.053 · Cited by 3,164
Fusobacterium nucleatum Potentiates Intestinal Tumorigenesis and Modulates the Tumor-Immune Microenvironment
Kostic, A. D., Chun, E., Robertson, L., Glickman, J. N., Gallini, C. A., Michaud, M., Clancy, T. E., Chung, D. C., Lochhead, P., Hold, G. L., et al. (2013) · Cell Host & Microbe · doi:10.1016/j.chom.2013.07.007 · Cited by 2,718
Fusobacterium nucleatum Promotes Colorectal Carcinogenesis by Modulating E-Cadherin/β-Catenin Signaling via its FadA Adhesin
Rubinstein, M. R., Wang, X., Liu, W., Hao, Y., Cai, G., & Han, Y. W. (2013) · Cell Host & Microbe · doi:10.1016/j.chom.2013.07.012 · Cited by 2,476
Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma
Castellarin, M., Warren, R. L., Freeman, J. D., Dreolini, L., Krzywinski, M., Strauss, J., Barnes, R., Watson, P., Allen-Vercoe, E., Moore, R. A., et al. (2012) · Genome Research · doi:10.1101/gr.126516.111 · Cited by 2,043
Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set
Nadkarni, M. A., Martin, F. E., Jacques, N. A., & Hunter, N. (2002) · Microbiology · doi:10.1099/00221287-148-1-257 · Cited by 1,931
An increase in the Akkermansia spp. population induced by metformin treatment improves glucose homeostasis in diet-induced obese mice
Shin, N.-R., Lee, J.-C., Lee, H.-Y., Kim, M.-S., Whon, T. W., Lee, M.-S., & Bae, J.-W. (2014) · Gut · doi:10.1136/gutjnl-2012-303839 · Cited by 1,550

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Zhang, B., Xu, S., Xu, W., Chen, Q., Chen, Z., Yan, C., Fan, Y., Zhang, H., Liu, Q., Yang, J., et al. (2019) · Frontiers in Genetics · doi:10.3389/fgene.2019.00447 · Cited by 20
IP6 reduces colorectal cancer metastasis by mediating the interaction of gut microbiota with host genes
Lan, T., Song, Y., Liu, X., Liu, C., Zhao, H.-C., Han, Y.-S., Wang, C.-H., Yang, N., Xu, Z., Meng, T., et al. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.979135 · Cited by 20
Tissue vs. Fecal-Derived Bacterial Dysbiosis in Precancerous Colorectal Lesions: A Systematic Review
Valciukiene, J., Strupas, K., & Poškus, T. (2023) · Cancers · doi:10.3390/cancers15051602 · Cited by 18
Clostridium ramosum rapidly identified by MALDI-TOF MS. A rare gram-variable agent of bacteraemia.
Legaria, M. C., García, S. D., Tudanca, V., Barberis, C. M., Cipolla, L., Cornet, L., Famiglietti, A. M. R., Stecher, D., & Vay, C. A. (2020) · Access Microbiology · doi:10.1099/acmi.0.000137 · Cited by 18
Leveraging Fecal Microbial Markers to Improve the Diagnostic Accuracy of the Fecal Immunochemical Test for Advanced Colorectal Adenoma
Zhang, Y., Lu, M., Lu, B., Liu, C., Ma, Y., Liu, L., Miao, X., Qin, J., Chen, H., & Dai, M. (2020) · Clinical and Translational Gastroenterology · doi:10.14309/ctg.0000000000000389 · Cited by 18
A metagenomic assessment of gut microbiota in Indian colon cancer patients
Bamola, V. D., Kapardar, R., Lal, B., Sharma, A., & Chaudhry, R. (2022) · Journal of Cancer Research and Therapeutics · doi:10.4103/0973-1482.341139 · Cited by 20
The Emerging Roles of Human Gut Microbiota in Gastrointestinal Cancer
Guo, Q., Qin, H., Liu, X., Zhang, X., Chen, Z., Qin, T., Chang, L., & Zhang, W. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.915047 · Cited by 21
Periodontitis and progression of gastrointestinal cancer: current knowledge and future perspective
Nasiri, K., Moghaddam, M. A., Etajuri, E. A., Badkoobeh, A., Tavakol, O., Rafinejad, M., Mirhosseini, A. F., & Fathi, A. (2023) · Clinical & Translational Oncology · doi:10.1007/s12094-023-03162-0 · Cited by 19
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Cao, Y., Nguyen, L. H., Tica, S., Otegbeye, E. E., Zong, X., Roelstraete, B., Chan, A. T., Warner, B. B., Stephansson, O., & Ludvigsson, J. F. (2023) · JAMA Network Open · doi:10.1001/jamanetworkopen.2023.10316 · Cited by 18
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Jonaitis, P., Kiudelis, V., Streleckienė, G., Gedgaudas, R., Skiecevičienė, J., & Kupčinskas, J. (2021) · Digestive Diseases · doi:10.1159/000515522 · Cited by 17
Diet, Microbes, and Cancer Across the Tree of Life: a Systematic Review
Kapsetaki, S. E., Alcaraz, G. M., Maley, C. C., Whisner, C. M., & Aktipis, A. (2022) · Current Nutrition Reports · doi:10.1007/s13668-022-00420-5 · Cited by 19
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Brim, H., Yooseph, S., Lee, E. W., Sherif, Z. A., Abbas, M. M., Laiyemo, A. O., Varma, S., Torralba, M., Dowd, S. E., Nelson, K., et al. (2017) · Genes · doi:10.3390/genes8110314 · Cited by 17
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Pratt, M., Forbes, J. D., Knox, N., Domselaar, G. V., & Bernstein, C. N. (2022) · Gastroenterology · doi:10.1053/j.gastro.2021.12.287 · Cited by 19
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Søreide, K. (2017) · Gastroenterology · doi:10.1053/j.gastro.2017.04.028 · Cited by 17
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Wolf, M., Steinberg, T., Scholz, K. J., Kruse, A., Rezasoltani, S., Conrads, G., Al‐Ahmad, A., & Cieplik, F. (2025) · Current Research in Microbial Sciences · doi:10.1016/j.crmicr.2025.100414 · Cited by 15
Candida albicans synergizes with Fusobacterium nucleatum in colorectal cancer progression via the Flo9-RadD interaction
Li, J., Zhang, C., Huang, X., Sun, H., Yang, L., Liu, H., Zhang, L., Wu, N., Xie, Y., Qi, J., et al. (2026) · Cancer Cell · doi:10.1016/j.ccell.2026.02.001 · Cited by 19
The association between Fusobacterium nucleatum and cancer colorectal: A systematic review and meta-analysis
Villar-Ortega, P., Ruíz, M. E., Gutiérrez-Soto, M., Jiménez, M. R.-C., Navarro‐Marí, J. M., & Fernández, J. G. (2022) · Enfermedades infecciosas y microbiologia clinica (English ed ) · doi:10.1016/j.eimce.2022.02.007 · Cited by 17
16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population
Rezasoltani, S., Looha, M. A., Aghdaei, H. A., Jasemi, S., Sechi, L. A., Gazouli, M., Sadeghi, A., Torkashvand, S., Baniali, R., Schlüter, H., et al. (2024) · Gut Pathogens · doi:10.1186/s13099-024-00604-0 · Cited by 15
Fecal fusobacterium nucleatum for detecting colorectal cancer: a systematic review and meta-analysis
Huang, Q., Peng, Y., & Xie, F. (2018) · The International Journal of Biological Markers · doi:10.1177/1724600818781301 · Cited by 16
Machine learning on microbiome research in gastrointestinal cancer
Cheung, H., & Yu, J. Q. (2021) · Journal of Gastroenterology and Hepatology · doi:10.1111/jgh.15502 · Cited by 16
Gut bacteria are required for the benefits of black raspberries in ApcMin/+ mice
Pan, P., Oshima, K., Huang, Y., Yearsley, M., Zhang, J., ARNOLD, M. W., Yu, J., & Wang, L.-S. (2018) · Journal of Berry Research · doi:10.3233/jbr-180337 · Cited by 15
Gut dysbiosis in cutaneous T‐cell lymphoma is characterized by shifts in relative abundances of specific bacterial taxa and decreased diversity in more advanced disease
Hooper, M. J., LeWitt, T. M., Pang, Y., Veon, F. L., Chlipala, G. E., Feferman, L., Green, S. J., Sweeney, D., Bagnowski, K., Burns, M. B., et al. (2022) · Journal of the European Academy of Dermatology and Venereology · doi:10.1111/jdv.18125 · Cited by 16
The fecal metabolome is associated with gestational diabetes mellitus
Liang, S., Hou, Z., Li, X., Wang, J., Cai, L., Zhang, R., & Li, J. (2019) · RSC Advances · doi:10.1039/c9ra05569j · Cited by 16
Oral and Fecal Microbiota in Lynch Syndrome
Ferrarese, R., Zuppardo, R. A., Puzzono, M., Mannucci, A., Amato, V., Ditonno, I., Patricelli, M. G., Raucci, A. R., Clementi, M., Elmore, U., et al. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm9092735 · Cited by 14
Role of salt‑inducible kinase 2 in the malignant behavior and glycolysis of colorectal cancer cells
Ni, X., Feng, Y., & Fu, X. (2021) · Molecular Medicine Reports · doi:10.3892/mmr.2021.12460 · Cited by 14
Analyses of publicly available Hungatella hathewayi genomes revealed genetic distances indicating they belong to more than one species
Hernández-Juárez, L. E., Camorlinga, M., Méndez-Tenorio, A., Flores-Calderón, J., Huang, B. C., Bandoy, D. D. R., Weimer, B. C., & Torres, J. (2021) · Virulence · doi:10.1080/21505594.2021.1950955 · Cited by 14
Engineering molecular theranostic probes for antibacterial therapy
Wang, X., Gong, J., Wang, J., Zhang, F., Mao, Z., & Liu, Z. (2023) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2023.117232 · Cited by 14
Biomarkers of inflammation and colorectal cancer risk
Li, Y., Luo, Y., Ran, Y., Lu, F., & Qin, Y. (2025) · Frontiers in Oncology · doi:10.3389/fonc.2025.1514009 · Cited by 13
Fusobacterium nucleatum in Health and Disease
Yang, X., Zhang, S., Ning, T., & Wu, J. (2025) · MedComm · doi:10.1002/mco2.70465 · Cited by 15
Determination of biomarkers associated with neoadjuvant treatment response focusing on colibactin-producing Escherichia coli in patients with mid or low rectal cancer: a prospective clinical study protocol (MICARE)
Taoum, C., Carrier, G., Jarlier, M., Roche, G., Gagnière, J., Fiess, C., de Forges, H., Chevarin, C., Colombo, P., Barnich, N., et al. (2022) · BMJ Open · doi:10.1136/bmjopen-2022-061527 · Cited by 12

Authors: Liang, Q., Chiu, J., Chen, Y., Huang, Y., Higashimori, A., Fang, J., Brim, H., Ashktorab, H., Ng, S. C., Ng, S. S. M., Zheng, S., Chan, F. K. L., Sung, J. J. Y., & Yu, J.
Affiliations: Chinese University of Hong Kong (HK)
Shanghai Jiao Tong University (CN)
Second Affiliated Hospital of Zhejiang University (CN)
1Institute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China.
2Division of Gastroenterology & Hepatology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Howard University (US)
Corresponding author: Liang, Qiaoyi
Yu, Jun
ORCID: Liang, Qiaoyi
Chen, Yingxuan
Huang, Yanqin
Brim, Hassan
Ashktorab, Hassan
Ng, Siew Chien
Ng, Simon Siu Man
Zheng, Shu
Chan, Francis Ka Leung
Sung, Joseph Jao Yiu
Yu, Jun
Venue: Clinical Cancer Research
Publisher: American Association for Cancer Research (AACR)
Published: 2017-04-13
Volume: 23
Issue: 8
Pages: 2061-2070
ISSN: 1078-0432 (ISSN-L)
1557-3265
DOI: 10.1158/1078-0432.ccr-16-1599
OpenAlex: W2528647554
Cited by: 332
References: 31
Citations by year: 2026: 28 · 2025: 25 · 2024: 29 · 2023: 37 · 2022: 44 · 2021: 45 · 2020: 41 · 2019: 33 · 2018: 32 · 2017: 16
FWCI: 14.37
Type: journal article
Language: en
Open access: closed
Locations: Clinical Cancer Research (publishedVersion)
PubMed (publishedVersion)
Funders: China 863 program (2012AA02A506)
China 973 program (2013CB531401)
National Nature and Science Foundation of China (81272304)
The National Key Technology R&D Program (2014BAI09B05)
Shenzhen Municipal Science and Technology R&D funding (JCYJ20130401151108652)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Health Sciences › Medicine › Oncology › Colorectal Cancer Screening and Detection
Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Keywords: colorectal cancer, fecal bacteria, non-invasive diagnosis, biomarkers, gut microbiota, Fusobacterium nucleatum, Roseburia intestinalis, metagenomics, fecal immunochemical test, cancer screening
MeSH terms: Gastrointestinal Microbiome, Adult, Aged, Feces, Female, Humans, Male, Middle Aged, ROC Curve, Sensitivity and Specificity, Biomarkers, Tumor, Colorectal Neoplasms, Area Under Curve, Real-Time Polymerase Chain Reaction

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Fecal Bacteria Act as Novel Biomarkers for Noninvasive Diagnosis of Colorectal Cancer

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