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The ATM Substrate KAP1 Controls DNA Repair in Heterochromatin: Regulation by HP1 Proteins and Serine 473/824 Phosphorylation
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Abstract
The repair of DNA damage in highly compact, transcriptionally silent heterochromatin requires that repair and chromatin packaging machineries be tightly coupled and regulated. KAP1 is a heterochromatin protein and co-repressor that binds to HP1 during gene silencing but is also robustly phosphorylated by Ataxia telangiectasia mutated (ATM) at serine 824 in response to DNA damage. The interplay between HP1-KAP1 binding/ATM phosphorylation during DNA repair is not known. We show that HP1α and unmodified KAP1 are enriched in endogenous heterochromatic loci and at a silent transgene prior to damage. Following damage, γH2AX and pKAP1-s824 rapidly increase and persist at these loci. Cells that lack HP1 fail to form discreet pKAP1-s824 foci after damage but levels are higher and more persistent. KAP1 is phosphorylated at serine 473 in response to DNA damage and its levels are also modulated by HP1. Unlike pKAP1-s824, pKAP1-s473 does not accumulate at damage foci but is diffusely localized in the nucleus. While HP1 association tempers KAP1 phosphorylation, this interaction also slows the resolution of γH2AX foci. Thus, HP1-dependent regulation of KAP1 influences DNA repair in heterochromatin. Mol Cancer Res; 10(3); 401–14. ©2011 AACR.
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“post-translational modification of KAP1 plays a critical role in determining which cellular context the protein will function in; for example sumoylated KAP1 promotes regulation of gene expression, while phosphorylated KAP1 promotes DNA repair41,42.”
From SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression fails with stress and age (2014) · cites this paper as [42]
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“analysis of promoter arrays41,42 showed that SIRT6 only co-occupies approximately 10% of KAP1 bound promoters”
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Metadata
- Authors
- White, D., Rafalska-Metcalf, I. U., Ivanov, A. V., Corsinotti, A., Peng, H., Lee, S.-C., Trono, D., Janicki, S. M., & Rauscher, F. J.
- Affiliations
- The Wistar Institute (US)
- Corresponding author
- Rauscher, Frank J.
- Venue
- Molecular Cancer Research
- Published
- 2012-03-01
- Volume
- 10
- Issue
- 3
- Pages
- 401-414
- ISSN
- 1541-7786 (ISSN-L)
- 1557-3125
- PMC
- PMC4894472
- OpenAlex
- W2100197820
- Cited by
- 152
- References
- 37
- Citations by year
- 2026: 11 · 2025: 12 · 2024: 15 · 2023: 9 · 2022: 7 · 2021: 14 · 2020: 9 · 2019: 8 · 2018: 8 · 2017: 12 · 2016: 11 · 2015: 14 · 2014: 13 · 2013: 4 · 2012: 3
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular Cancer Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Funders
- National Cancer Institute (R01CA129833, CA010815, CA129833, CA126283, F32CA126283, P30CA010815)
- National Center for Research Resources (P20 RR16440, P20RR016440)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- KAP1, ATM, HP1, heterochromatin, DNA repair, γH2AX foci, gene silencing, chromatin condensation, DNA damage response
- MeSH terms
- Tripartite Motif-Containing Protein 28, Chromobox Protein Homolog 5, Amino Acid Sequence, Animals, Cell Fractionation, Chromosomal Proteins, Non-Histone, DNA Repair, DNA-Binding Proteins, Heterochromatin, Histones, Humans, Immunohistochemistry, Models, Biological, Molecular Sequence Data, Nuclear Proteins, Phosphorylation, Phosphoserine, Repressor Proteins, Substrate Specificity, Blotting, Western, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Transgenes, Tumor Suppressor Proteins, NIH 3T3 Cells, Mutant Proteins, Mice, Gene Knockdown Techniques, Ataxia Telangiectasia Mutated Proteins
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