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Cited by 21Hypothalamic control of reproductive hormonesNeuroendocrine regulation and behaviorNeuropeptides and Animal Physiology

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Central Stimulatory Influence of Oxytocin on Preovulatory Gonadotropin-Releasing Hormone Requires More than the Median Eminence

Selvage, Daniel, Johnston, Craig A.·2001·Neuroendocrinology
DOI 10.1159/000054678
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Abstract

The study was designed to determine whether the ability of central oxytocin (OT) to stimulate gonadotropin-releasing hormone (GnRH) on the afternoon of proestrus (PE) in the cycling female rat is mediated at the level of GnRH terminals within the median eminence (ME), or at higher hypothalamic levels where GnRH cell bodies and axons are located. Determining the location of this OT effect in vivo has proven difficult. Therefore, an in vitro system utilizing ME or basal hypothalamic (BH) explants containing GnRH terminals, or GnRH neurons including the cell bodies, axons and terminals, respectively, were harvested from regular cycling female rats at 15:00 h on PE or diestrus (DI). The explants were allowed to preincubate in Krebs Ringer Bicarbonate Buffer containing glucose, ascorbic acid, calcium, and a metalloprotease inhibitor (KRBG) and enriched with 95% O 2/5% CO 2 at 37°C until a stable baseline release of GnRH was achieved (30 min). The 0.05 level of probability was used as the minimum criterion of significance in all experiments. The ability of OT (10–15–10–9M) to stimulate the release of GnRH was determined in both ME and BH explants on PE and DI. The results demonstrated a sensitive, dose-dependent ability of OT to stimulate GnRH release from PE BH explants which was observed only in PE. Furthermore, OT failed to significantly stimulate GnRH release from ME explants on either PE or DI. The data indicate that the PE BH explant paradigm can be used to examine the manner and mechanisms by which OT influences GnRH release on the afternoon of PE. Furthermore, the results indicate for the first time that the stimulatory action of OT by itself on preovulatory GnRH release in cycling female rats is not mediated at the level of the GnRH terminals within the ME, but requires neuronal interactions and mechanisms within the BH explants.

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Metadata

Authors
Selvage, D., & Johnston, C. A.
Affiliations
University of Montana (US)
ORCID
Johnston, Craig A.
Venue
Neuroendocrinology
Publisher
S. Karger AG
Published
2001
Volume
74
Issue
2
Pages
129-134
ISSN
  • 0028-3835 (ISSN-L)
  • 1423-0194
DOI
10.1159/000054678
OpenAlex
W2059720695
Cited by
20
References
21
Citations by year
2026: 1 · 2020: 2 · 2017: 2 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 1
FWCI
0.71
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Neuroendocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p20rr011796-03)
  • National Center for Research Resources (P20RR11796)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroendocrine regulation and behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
Keywords
oxytocin, gonadotropin-releasing hormone, median eminence, basal hypothalamus, GnRH release, proestrus, hypothalamic explants, GnRH neurons, female rats, menstrual cycle, neuroendocrine regulation of reproduction
MeSH terms
Animals, Axons, Female, Hypothalamus, Gonadotropin-Releasing Hormone, Median Eminence, Nerve Endings, Neurons, Oxytocin, Proestrus, Rats, Sprague-Dawley, Rats, In Vitro Techniques

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