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Repeated stress–induced activation of corticotropin–releasing factor neurons enhances vasopressin stores and colocalization with corticotropin–releasing factor in the median eminence of rats
Abstract
Stress-induced release of corticotropin-releasing factor (CRF) and vasopressin (AVP) was studied in rats by measuring the decline of CRF and AVP stores in the median eminence after blockade of fast axonal transport with colchicine (5 micrograms per rat intracisternally). Quantitative immunocytochemistry was used to detect changes in CRFi and AVPi in the external zone of the median eminence (ZEME) selectively. Immobilization stress induced a fast ACTH response to 1,000-2,000 pg/ml which was associated with a fall in both CRFi and AVPi of 34% during the first 30 min. This is followed by different time courses of further AVPi and CRFi depletion. In addition, we investigated the effect of repeated daily stress exposure on CRFi and AVPi in the ZEM 1 day after stress exposure. Repeated daily immobilization for 9 or 16 subsequent days did not affect the CRFi stores in the ZEME, but increased the AVPi stores to 161 +/- 13% and 218 +/- 11% respectively. Quantitative analysis of electron microphotographs of repeatedly handled rats showed a mean density of CRF positive profiles in the ZEME of 45.5 +/- 2.5 per 500 microns 2 of which 25% also stained for pro-AVP-derived peptides. After 9 subsequent days of immobilization the total density of CRF-positive profiles remained unchanged, but the fraction of CRF swellings that also stained for pro-AVP-derived peptides increased approximately 2-fold. We conclude that (1) the secretion of AVPi and CRFi from the ZEME are independently controlled, indicating differential activation of AVP containing and AVP deficient CRF neurons during acute immobilization, and (2) repeated stress leads to plastic changes in hypothalamic CRF neurons resulting in increased AVP stores and colocalization in CRF nerve terminals.
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“Additionally, during stress, particularly chronic stress and after adrenalectomy, the number of parvocellular CRH neurosecretory cells that co-produce AVP was found to increase considerably as do the amounts of synthesized AVP compared to CRH per cell (66, 67).”
From Influence of arginine vasopressin on the ultradian dynamics of Hypothalamic-Pituitary-Adrenal axis (2022) · cites this paper as [67]
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Self-citation
Metadata
- Authors
- de Goeij, D. C. E., Kvetnansky, R., Whitnall, M. H., Jezova, D., Berkenbosch, F., & Tilders, F. J. H.
- Venue
- Neuroendocrinology
- Publisher
- S. Karger AG
- Published
- 2008-04-04
- Volume
- 53
- Issue
- 2
- Pages
- 150-159
- ISSN
- 0028-3835 (ISSN-L)
- 1423-0194
- OpenAlex
- W2083555314
- Cited by
- 250
- References
- 0
- Citations by year
- 2025: 2 · 2023: 3 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 2 · 2017: 2 · 2016: 11 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 5
- FWCI
- 3.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroendocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroendocrine regulation and behavior
- Keywords
- corticotropin-releasing factor, vasopressin, median eminence, restraint stress, repeated stress, CRF neurons, colchicine, fast axonal transport, ACTH
- MeSH terms
- Adrenocorticotropic Hormone, Animals, Arginine Vasopressin, Colchicine, Corticotropin-Releasing Hormone, Kinetics, Male, Median Eminence, Neurons, Rats, Inbred Strains, Restraint, Physical, Stress, Physiological, Rats
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