The pulsatility of ACTH secretion in the rat anterior pituitary cell perifusion system
Open accessAbstract
Aims: This study aimed to examine the physiological mechanism whereby the corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) exert their influence on adrenocorticotropic hormone (ACTH) secretion in pituitary cells. Methods: Anterior pituitary cells were harvested from male rats and placed in the perifusion system. Cells were perifused with serum-free medium for 6 hours before fraction collection. After 30-minute of baseline collection, perifusion media was changed to expose the cells to CRH with or without AVP. ACTH concentration in each fraction was measured using enzyme immunoassay chemiluminescent kit. Results: The lowest physiological concentration of CRH (10 pM) or AVP (10 pM) was not able to induce a marked increase in ACTH secretion. Higher concentration of CRH (30 pM) or AVP (100 pM) in the physiological range caused sustained elevation of ACTH secretion (P < 0.001), while the secretion remained at similar levels for up to 1 hour with continuous stimulation. Perifusion with 10 pM AVP and 10 pM or 30 pM CRH caused a 2.38-fold and 2.99-fold increase in pulsatile ACTH secretion in pituitary cells, respectively. The duration of pulsatility caused by perifusion with 10 pM AVP and 30 pM CRH was close to that observed under physiological condition. Conclusions: By using the rat anterior pituitary cell perifusion system, we found that CRH and AVP potentiate the effect of each other on ACTH secretion, but AVP was a less potent agonist than CRH. The data suggest that CRH and AVP are essential for the pulsatility of ACTH, and AVP acts like a switch of the pulsatility.
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CRH and AVP are capable of potentiating each other's activity.
“Moreover, CRH and AVP are also capable of potentiating each other’s activity (37, 38).”
From Influence of arginine vasopressin on the ultradian dynamics of Hypothalamic-Pituitary-Adrenal axis (2022) · cites this paper as [38]
Synergistic stimulation of ACTH production and secretion by corticotrope cells by CRH and AVP could yield a several-fold higher output than either one individually.
“Their synergistic stimulation of ACTH production and secretion by corticotrope cells could yield a several-fold higher output than due to the action of either one individually (26, 34–41, 43–47).”
From Influence of arginine vasopressin on the ultradian dynamics of Hypothalamic-Pituitary-Adrenal axis (2022) · cites this paper as [38]
CRH and AVP are both required as stimulatory inputs to corticotrope cells for a complete ACTH response in physiologically normal and various stressful conditions.
“In essence, CRH and AVP are both required as stimulatory inputs to corticotrope cells for a complete ACTH response in physiologically normal as well as in various stressful conditions (32, 38, 48–51).”
From Influence of arginine vasopressin on the ultradian dynamics of Hypothalamic-Pituitary-Adrenal axis (2022) · cites this paper as [38]
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“There are results in several studies in vitro in rat anterior pituitary cells and in vivo in rats, indicating the necessity for much higher concentrations of exogenously administered AVP in order to induce similar effects on ACTH secretion as certain injected CRH concentrations (37, 38, 40, 41).”
BackgroundResultAMOKRANE-FERRAH, A., ANANE, A., Boukenaoui-Ferrouk, N., Khaldoun, M., Amirat, Z., Mormède, P., & Khammar, F. (2022) · Biodiversitas Journal of Biological Diversity · doi:10.13057/biodiv/d230140
Stojiljković, A., Čupić, Ž., Maćešić, S. R., Ivanović-Šašić, A., & Mojović, M. (2025) · Royal Society Open Science · doi:10.1098/rsos.251323
- Authors
- Deng, Q., Zhang, Z., & Wu, Y.
- Affiliations
- Peking University Shenzhen Hospital (CN)
- Anhui Medical University (CN)
- Venue
- Cellular Physiology and Biochemistry
- Publisher
- S. Karger AG
- Published
- 2017
- Volume
- 41
- Issue
- 1
- Pages
- 154-162
- ISSN
- 1015-8987 (ISSN-L)
- 1421-9778
- OpenAlex
- W2594337357
- Cited by
- 6
- References
- 39
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 2 · 2021: 1 · 2017: 1
- FWCI
- 0.15
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cellular Physiology and Biochemistry (publishedVersion, cc-by-nc-nd)
- Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- China Postdoctoral Science Foundation
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- ACTH secretion, pulsatile hormone secretion, corticotropin-releasing factor, vasopressin, anterior pituitary cells
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