Effect of Fasting on Uteroplacental Amino Acid Metabolism in the Pregnant Sheep
Abstract
In the fed state, the net utilization of most amino acids by the ovine uteroplacental unit (defined as uterine uptake minus umbilical uptake) is zero. This study was to determine if this is true for the fasted pregnant ewe also, when uteroplacental glucose uptake is diminished. Glucose and amino acid concentrations were determined in 9 pregnant ewes across the uterine and umbilical circulations. Uterine and umbilical blood flows were determined by the steady state antipyrine diffusion method, and net uptakes across each circulation calculated by application of the Fick principle. Five amino acids were found to have a consistently greater uterine uptake than umbilical uptake. In addition, glutamate had a negative net umbilical uptake coupled with no net uterine uptake. Fasting resulted in an increase in the differences between the respective uptakes for all five amino acids. No additional amino acids were found to have disparate uptakes than were seen in the fed state. Umbilical glutamate uptake became less negative during fasting, but the difference between the uterine and umbilical circulations remained unchanged, as the uterine circulation took up glutamate during fasting. The umbilical circulation took up α-ketoisocaproate (0.626 ± 0.18, fed), and the uptake was increased during fasting (1.8 ± 0.6). These findings suggest that the branched chain amino acids and glutamine are utilized by the ovine uteroplacenta to a greater degree than are most other amino acids, and at increased rates during fasting of the ewe. These amino acids, through deamination, may be the source of uteroplacental ammonia production, as well as serving as an energy substrate when glucose supply is limited.
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There are increases in the uterine uptake and uteroplacental utilisation of some branched chain amino acids in response to fasting ewes, which may provide an alternative source of energy.
“There are increases in the uterine uptake and uteroplacental utilisation of some branched chain amino acids in response to fasting ewes, which may provide an alternative source of energy (338).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [338]
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Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation.
Bell, A. W. (1995) · Journal of Animal Science · doi:10.2527/1995.7392804x · Cited by 1,363 · see citing works
Placental Origins of Chronic Disease
Burton, G. J., Fowden, A. L., & Thornburg, K. L. (2016) · Physiological Reviews · doi:10.1152/physrev.00029.2015 · Cited by 717 · see citing works
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Bell, A. W., & Ehrhardt, R. (2002) · Nutrition Research Reviews · doi:10.1079/nrr200239 · Cited by 161 · see citing works
“Thus, the appreciable net consumption of glutamate, serine, and branched-chain amino acids by the placenta (Liechty et al .”
BackgroundPlacental transport and fetal utilization of leucine in a model of fetal growth retardation
J. C. Ross, Fennessey, P. V., Wilkening, R. B., Battaglia, F. C., & Meschia, G. (1996) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1996.270.3.e491 · Cited by 146 · see citing works
Bell, A. W., Slepetis, R. M., & Ehrhardt, U. A. (1995) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(95)76821-7 · Cited by 146 · see citing works
Production and utilization of amino acids by ovine placenta in vivo
Chung, M., Teng, C. C., Timmerman, M., Meschia, G., & Battaglia, F. C. (1998) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1998.274.1.e13 · Cited by 104 · see citing works
“Thus, in the previous comparison of uterine and umbilical uptakes (15), glycine concentrations were not measured despite the significant contribution that glycine makes to umbilical uptake of amino acids (7).”
BackgroundResultGlutamine-glutamate exchange between placenta and fetal liver
Vaughn, P. R., Lobo, C., Battaglia, F. C., Fennessey, P. V., Wilkening, R. B., & Meschia, G. (1995) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1995.268.4.e705 · Cited by 102 · see citing works
Placental metabolism: substrate requirements and the response to stress
Vaughan, O. R., & Fowden, A. (2016) · Reproduction in Domestic Animals · doi:10.1111/rda.12797 · Cited by 74 · see citing works
Leucine metabolism in chronically hypoglycemic hypoinsulinemic growth-restricted fetal sheep
Carver, T., Quick, A. A., Teng, C. C., Pike, A. W., Fennessey, P. V., & Hay, W. W. (1997) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1997.272.1.e107 · Cited by 48 · see citing works
Bloomfield, F. H., van Zijl, P. L., Bauer, M. K., & Harding, J. E. (2002) · Journal of Pediatric Gastroenterology and Nutrition · doi:10.1097/00005176-200209000-00010 · Cited by 35 · see citing works
“During maternal fasting (64), or when placental growth is restricted by carunclectomy (60), placental uptake of branched chain amino acids is increased, and in the latter case this may be at the expense of the fetus.”
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Noorlander, C. W., Graan, P. N. E. D., Nikkels, P. G. J., et al. (2003) · Placenta · doi:10.1016/j.placenta.2003.10.018 · Cited by 34 · see citing works
Regulation of glutamate transport and transport proteins in a placental cell line
Novak, D. A., Quiggle, F., Artime, C., et al. (2001) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.2001.281.3.c1014 · Cited by 31 · see citing works
“” In this cycle, which has been carefully defined in the ovine model, maternal glutamine is transferred across the placenta to the fetal circulation, extracted by the fetal liver, and then deaminated for nitrogen utilization and release of glutamate (2, 22, 26, 31, 45).”
BackgroundWaterman, R. C., Ujazdowski, V. L., & Petersen, M. K. (2012) · Amino Acids · doi:10.1007/s00726-012-1301-3 · Cited by 26 · see citing works
Chapter 1 Regulation of metabolism and growth during prenatal life
Bell, A. W., Greenwood, P. L., & Ehrhardt, R. (2005) · Biology of growing animals · doi:10.1016/s1877-1823(09)70008-6 · Cited by 25 · see citing works
Timmerman, M., Chung, M., Wilkening, R. B., et al. (1998) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1998.275.6.e942 · Cited by 25 · see citing works
“In sheep, alanine is transported to the pregnant uterus (3, 7) and from the placenta to the fetus (2, 6, 10).”
BackgroundKatz, S. G. (1995) · Tissue and Cell · doi:10.1016/s0040-8166(05)80026-6 · Cited by 15 · see citing works
McCoard, S. A., Haack, N. A., Heiser, A., & Maclean, P. H. (2023) · Theriogenology · doi:10.1016/j.theriogenology.2023.03.009 · Cited by 9 · see citing works
Response of placental amino acid transport to gestational age and intrauterine growth retardation
Matthews, J. C., Beveridge, M. J., Malandro, M. S., et al. (1998) · Proceedings of The Nutrition Society · doi:10.1079/pns19980040 · Cited by 9 · see citing works
Extracellular breakdown of collagen by mice decidual cells. A cytochemical and ultrastructural study
Katz, S. G. (2005) · Biocell · doi:10.32604/biocell.2005.29.261 · Cited by 7 · see citing works
“There is a prompt fall in fetal glucose concentrations and, to compensate, an increase in available amino acids (Simmons et al., 1974; Johnson et al., 1986; Liechty et al., 1991).”
BackgroundGlutamate Transport by Rcho-1 Cells Derived From Rat Placenta
Novak, D. A., & Matthews, J. C. (2003) · Pediatric Research · doi:10.1203/01.pdr.0000061542.06874.2a · Cited by 6 · see citing works
Differentially expressed genes in cotyledon of ewes fed mycotoxins
Britt, J. L., Noorai, R. E., & Duckett, S. K. (2020) · BMC Genomics · doi:10.1186/s12864-020-07074-z · Cited by 4 · see citing works
Urine Metabolome during Parturition
Gevi, F., Meloni, A., Mereu, R., Lelli, V., Chiodo, A., Ragusa, A., & Timperio, A. M. (2020) · Metabolites · doi:10.3390/metabo10070290 · Cited by 5 · see citing works
“The net fluxes of glutamine into the pregnant uterus and into the fetus are large in comparison with the net fluxes of other amino acids [35,36].”
BackgroundBloomfield, F. H., & Harding, J. E. (2006) · Cambridge University Press eBooks · doi:10.1017/cbo9780511544712.002 · Cited by 7 · see citing works
Grisaru‐Granovsky, S., Hamudi, N., Samueloff, A., et al. (2008) · The Open Women s Health Journal · doi:10.2174/1874291200802010001 · Cited by 1 · see citing works
“The pathologic maternal chronic hyperglycemic/ hypoglycemic on fetal hormonal and behavior states were extensively studied [12, 13].”
BackgroundAndrogen Secretion by Rcho-1 Cells is Independent of Extracellular Glutamate Concentration
Novak, D., Quiggle, F., & Matthews, J. C. (2004) · Placenta · doi:10.1016/j.placenta.2003.12.003 · Cited by 1 · see citing works
“Placental glutamate is, in large part, derived from the fetal circulation where it exists as a product of the maternal/fetal ‘glutamine-glutamate cycle’ [9,10,12–14].”
BackgroundThongsong, B. (2013) · The Thai Journal of Veterinary Medicine · doi:10.56808/2985-1130.2479
“placenta to the fetal circulation (Liechty et al., 1991; Vaughn et al., 1995).”
in vivoand utilization of amino acids by ovine placenta
Chung, M., Teng, C. C., Timmerman, M., et al. (2016)
- Authors
- Liechty, E. A., Kelley, J., & Lemons, J. A.
- Affiliations
- Indiana University Indianapolis (US)
- Indiana University (US)
- ORCID
- Liechty, Edward A.
- Venue
- Biology of the Neonate
- Publisher
- S. Karger AG
- Published
- 2009-09-25
- Volume
- 60
- Issue
- 3-4
- Pages
- 207-214
- ISSN
- 0006-3126 (ISSN-L)
- 1421-9727
- OpenAlex
- W1980612945
- Cited by
- 27
- References
- 0
- Citations by year
- 2023: 1 · 2020: 2 · 2016: 3 · 2013: 1 · 2012: 1
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biology of the Neonate (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5k04hd000865-04, 5r01hd019089-17)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01-HD19089, KO4-HD00865)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Reproductive Physiology in Livestock
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Keywords
- fasting, uteroplacental circulation, amino acid metabolism, pregnant ewes, uterine blood flow, umbilical blood flow, amino acid uptake, glutamate, branched-chain amino acids, glutamine, alpha-ketoisocaproate, ammonia synthesis
- MeSH terms
- Amino Acids, Analysis of Variance, Animals, Blood Flow Velocity, Fasting, Female, Fetal Blood, Fetus, Glucose, Keto Acids, Maternal-Fetal Exchange, Oxygen, Placenta, Pregnancy, Pregnancy, Animal, Sheep, Uterus
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