Aberrant expression of splicing factors in newly diagnosed acute myeloid leukemia
Abstract
BACKGROUND: Acute myeloid leukemia (AML) is the most common type of blood cancer in adults. Emerging evidence is establishing a connection between AML and aberrant alternative splicing of pre-mRNA, which may result from aberrant expression of splicing factors, the mediators of splicing reactions. MATERIAL AND METHODS: Using quantitative real-time polymerase chain reaction, we measured mRNA expression of 7 splicing factors belonging to the serine/arginine-rich (SR) protein family, SRSF1 (SF2/ASF), SRSF2 (SC35), SRSF3 (SRp20), SRSF4 (SRp75), SRSF5 (SRp40), SRSF6 (SRp55), and SRSF7 (9G8), and 1 non-SR factor, heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), in peripheral blood mononuclear cells of 26 patients with newly diagnosed AML and 26 healthy controls. In addition, the relationship between splicing factors and the mRNA splicing patterns of the caspase-8 gene (CASP8) was investigated. RESULTS: Compared to healthy controls, the expression of splicing factors was obviously aberrant in newly diagnosed AML patients. The expression of SRSF1, SRSF3 and SRSF4 mRNAs was significantly decreased. Moreover, a significant correlation was observed between several splicing factors and caspase-8 pre-mRNA splicing in AML patients, but not in control subjects. CONCLUSION: These data suggest that aberrant expression of splicing factors in AML may potentially connect with abnormal expression of oncogenes and be useful for early diagnosis, prognosis, and therapy of AML.
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hnRNP K, SRSF3, and YB-1 show altered expression in AML and contribute to leukemia progression.
“In addition, the expression of hnRNP K (40), SRSF3 (SRp20) (41), and YB-1 (42) has been shown to be altered in AML, but also to contribute to leukemia progression.”
From Discovery of a Novel CIP2A Variant (NOCIVA) with Clinical Relevance in Predicting TKI Resistance in Myeloid Leukemias (2021) · cites this paper as [41]
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- Authors
- Liu, J., Huang, B., Xiao, Y., Xiong, H.-M., Li, J., Feng, D.-Q., Chen, X.-M., Zhang, H.-B., & Wang, X.-Z.
- Affiliations
- Second Affiliated Hospital of Nanchang University (CN)
- First Affiliated Hospital of Nanchang University (CN)
- Venue
- Onkologie
- Publisher
- S. Karger AG
- Published
- 2012
- Volume
- 35
- Issue
- 6
- Pages
- 335-340
- ISSN
- 0378-584X (ISSN-L)
- 1423-0240
- OpenAlex
- W2009001388
- Cited by
- 53
- References
- 21
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 6 · 2023: 6 · 2022: 4 · 2021: 8 · 2020: 4 · 2019: 3 · 2018: 6 · 2017: 6 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 1
- FWCI
- 0.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Onkologie (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Keywords
- acute myeloid leukemia, splicing factors, alternative splicing, SR proteins, SRSF1, SRSF3, hnRNP A1, caspase-8, pre-mRNA splicing, peripheral blood mononuclear cells, mRNA expression
- MeSH terms
- Adult, Aged, Female, Humans, Male, Middle Aged, Mutation, Oncogenes, RNA Splicing, Leukemia, Myeloid, Acute, Alternative Splicing, Genetic Predisposition to Disease, Young Adult
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