Expression of Amyloid-Associated miRNAs in Both the Forebrain Cortex and Hippocampus of Middle-Aged Rat
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Background Aging is associated with the gradual cognitive decline and shows the typical senile plaque formation in the brain, which results from the aggregation of beta amyloid (Aβ) peptide following the abnormal proteolytic processing of amyloid precursor protein (APP) by β-secretase (BACE1) and γ-secretase. Accumulating evidence indicates that several microRNAs (miRNAs) are involved in the Alzheimer's disease (AD) by regulating the expression of APP and BACE1 proteins. However, the cognitive ability and the expression profile of the APP- and BACE1-associated miRNAs in the middle-aged population are largely unknown. Methods The learning and memory ability in rats were determined by Morris Water Maze test. The protein levels of APP and BACE1 were detected by western blotting. The quantitative polymerase chain reaction was used to identify the miRNAs levels in forebrain cortex and the hippocampus. Results Middle-aged rats have declined learning ability without changes in the memory ability, and increased APP and BACE1 protein expression in the forebrain cortex. Computational analysis using Targetscan and Pictar databases reveals that totally 4 predicted miRNAs have conserved binding site with APP, namely miR-106b, -17-5p, -153, -101. All of them showed decreased expression in both the forebrain cortex and hippocampus. Among the 10 predicted miRNAs targeting BACE1, different expression profiles were identified in the forebrain cortex (decreased: miR-9, -19a, -135a, -15b, -16, -…
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Expression and activity of BACE1 are increased during aging in the brains of mice, rats and humans.
“Previous studies consistently reported that expression and activity of BACE1 are increased during aging in the brains mice, rats and humans (Boissonneault et al. 2009, Che et al. 2014, Fukumoto et al. 2004, Rossner et al. 2006), raising the possibility of a link between aging-associated alterations in BACE1 and the increased risk for AD.”
From miR‐186 is decreased in aged brain and suppresses <scp>BACE</scp>1 expression (2016) · cites this paper as Che et al. 2014
BACE1 protein levels are increased in the brains of mice and rats at middle age compared to young age.
“Previous reports showed that BACE1 protein levels are increased in the brains of mice and rats at middle age compared to young age (Boissonneault et al. 2009, Che et al. 2014).”
From miR‐186 is decreased in aged brain and suppresses <scp>BACE</scp>1 expression (2016) · cites this paper as Che et al. 2014
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RETRACTED ARTICLE: A specific amyloid-β protein assembly in the brain impairs memory
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RetractedmicroRNA Expression Patterns Reveal Differential Expression of Target Genes with Age
Noren Hooten, N., Abdelmohsen, K., Gorospe, M., Ejiogu, N., Zonderman, A. B., & Evans, M. K. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010724 · Cited by 357 · see citing works
β-Secretase Activity Increases with Aging in Human, Monkey, and Mouse Brain
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Synapses are lost during aging in the primate prefrontal cortex
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miR‐186 is decreased in aged brain and suppresses <scp>BACE</scp>1 expression
Kim, J., Yoon, H., Chung, D., Brown, J. L., Belmonte, K. C., & Kim, J. (2016) · Journal of Neurochemistry · doi:10.1111/jnc.13507 · Cited by 102 · see citing works
- Authors
- Che, H., Sun, L.-H., Guo, F., Niu, H.-F., Su, X.-L., Bao, Y.-N., Fu, Z. D., Liu, H.-L., Hou, X., Yang, B.-F., & Ai, J.
- Affiliations
- Harbin Medical University (CN)
- Venue
- Cellular Physiology and Biochemistry
- Publisher
- S. Karger AG
- Published
- 2014
- Volume
- 33
- Issue
- 1
- Pages
- 11-22
- ISSN
- 1015-8987 (ISSN-L)
- 1421-9778
- OpenAlex
- W2118629432
- Cited by
- 35
- References
- 6
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 6 · 2022: 4 · 2020: 3 · 2019: 3 · 2018: 2 · 2017: 3 · 2016: 5 · 2015: 4
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cellular Physiology and Biochemistry (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- Alzheimer's disease, amyloid precursor protein, BACE1, middle-aged rats, cognitive decline, learning and memory, hippocampus, miRNA expression profiling, miR-124, miR-106b, miR-17-5p, miR-153, miR-101, miR-19a, miR-135a, miR-15b, miR-16, miR-195, miR-29c, miR-214, miR-141, miRNA target prediction
- MeSH terms
- Aging, Amyloid, Animals, Cognition, Hippocampus, Male, RNA, Messenger, Aspartic Acid Endopeptidases, Amyloid beta-Protein Precursor, Rats, Wistar, Gene Expression Regulation, Developmental, Gene Expression Profiling, MicroRNAs, Amyloid Precursor Protein Secretases
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