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Delta-Aminolevulinic Acid Dehydratase Assay
Abstract
gamma-Aminolevulinic acid (ALA) dehydratase catalyzes the synthesis of porphobilinogen (PBG) from two molecules of ALA. A semimicro method for the colorimetric determination of ALA dehydratase is presented and applied to various tissues. The enzyme activity in adult male rat liver was 2.22 and 1.94 mumol PBG formed/g liver/h for homogenates assayed with or without dithiothreitol, respectively. The assay was linear for at least 2.5 h and for up to 2.5 mg tissue per assay. The Km for ALA was 4.0 X 10(-4)M and the pH optimum was 6.2-6.4. The effects of activators and inhibitors on enzyme activity are discussed.
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Cited by
Zhang, J., Kang, Z., Chen, J., & Du, G. (2015) · Scientific Reports · doi:10.1038/srep08584 · Cited by 148
Gibson, S., Cupriks, D., Havens, J., Nguyen, M., & Hilf, R. (1998) · British Journal of Cancer · doi:10.1038/bjc.1998.39 · Cited by 78
Hematological changes in rats chronically exposed to oral aluminum
Farina, M., Rotta, L. N., Soares, F. A. A., Jardim, F., Jacques, R., Souza, D. O., & Rocha, J. B. T. (2005) · Toxicology · doi:10.1016/j.tox.2004.12.005 · Cited by 37
The pH dependency of protoporphyrin IX formation in cells incubated with 5-aminolevulinic acid
Bech, Ø., Berg, K., & Moan, J. (1997) · Cancer Letters · doi:10.1016/s0304-3835(96)04558-2 · Cited by 32
Fuyuki, I., Galbraith, R. A., & Sassa, S. (1990) · International Journal of Biochemistry · doi:10.1016/0020-711x(90)90344-3 · Cited by 24
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Metadata
- Authors
- Sassa, S.
- Venue
- Enzyme
- Publisher
- S. Karger AG
- Published
- 1982
- Volume
- 28
- Issue
- 2-3
- Pages
- 133-145
- ISSN
- 0013-9432 (ISSN-L)
- 2504-2564
- OpenAlex
- W2400418602
- Cited by
- 437
- References
- 0
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- closed
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