The pH dependency of protoporphyrin IX formation in cells incubated with 5-aminolevulinic acid
Abstract
Chinese hamster cells (V79) and human adenocarcinoma cells (WiDr) were incubated with 5-aminolevulinic acid (ALA) at different pH values and the rate of production of protoporphyrin IX (PpIX) was measured. The rate of production increased with pH in the range 6.0-7.5. Above pH 7.5 the rate decreased, possibly due to a reduced metabolic activity of the cells. The observations may be explained by the known pH-dependency of the activity of porphobilinogen deaminase (PBGD) and are in agreement with the assumption that PBGD constitutes a rate-limiting step in the synthesis of PpIX from ALA.
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Other parameters that affect PpIX accumulation include pH, oxygen availability, illumination conditions, or thickness of stratum corneum.
“Other parameters that affect PpIX accumulation include pH [63,64,65], oxygen availability [63,66], illumination conditions, or thickness of striatum corneum [67].”
From Aminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer (2011) · cites this paper as [65]
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- Authors
- Bech, Ø., Berg, K., & Moan, J.
- Corresponding author
- Moan, J.
- ORCID
- Berg, K.
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 1997-02
- Volume
- 113
- Issue
- 1-2
- Pages
- 25-29
- ISSN
- 0304-3835 (ISSN-L)
- OpenAlex
- W2172152514
- Cited by
- 32
- References
- 28
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 2 · 2020: 1 · 2016: 1 · 2013: 1
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cancer Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Porphyrin Metabolism and Disorders
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Physical Sciences › Materials Science › Materials Chemistry › Porphyrin and Phthalocyanine Chemistry
- Keywords
- protoporphyrin IX, 5-aminolevulinic acid, pH dependence, porphobilinogen deaminase, heme biosynthesis, rate-determining step, V79 cells, cell metabolic activity
- MeSH terms
- Adenocarcinoma, Aminolevulinic Acid, Animals, Cell Survival, Colonic Neoplasms, Fibroblasts, Cricetinae, Humans, Hydrogen-Ion Concentration, Lung, Proteins, Protoporphyrins, Tumor Cells, Cultured
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