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Cited by 10Genetics and Neurodevelopmental DisordersAutism Spectrum Disorder ResearchWilliams Syndrome Research

Prenatal High-Fat Diet Rescues Communication Deficits in Fmr1 Mutant Mice in a Sex-Specific Manner

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Nolan, Suzanne O., Hodges, Samantha L., Okoh, James T., Binder, Matthew S., Lugo, Joaquin N.·2020·Developmental Neuroscience
DOI 10.1159/000509797PMC PMC7864857
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Abstract

Using high-throughput analysis methods, the present study sought to determine the impact of prenatal high-fat dietary manipulations on isolation-induced ultrasonic vocalization production in both male and female Fmr1mutants on postnatal day 9. Prior to breeding, male FVB/129 Fmr1 wildtype and female Fmr1 heterozygous breeding pairs were assigned to 1 of 3 diet conditions: standard lab chow, omega-3 fatty acid-enriched chow, and a diet controlling for the fat increase. Prenatal exposure to omega-3 fatty acids improved reductions in the number of calls produced by Fmr1heterozygotes females. Moreover, diminished spectral purity in the female Fmr1homozygous mouse was rescued by exposure to both high-fat diets, although these effects were not seen in the male Fmr1knockout. Prenatal dietary fat manipulation also influenced several other aspects of vocalization production, such as the number of calls produced and their fundamental frequency, aside from effects due to loss of Fmr1.Specifically, in males, regardless of genotype, prenatal exposure to high omega-3s increased the average fundamental frequency of calls. These data support the need for future preclinical and clinical work elucidating the full potential of prenatal high-fat diets as a novel therapeutic alternative forFragile X syndrome.

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  • n-3 polyunsaturated fatty acid supplementation has beneficial effects on the behavioral phenotype of Fmr1 KO mice.

    “n-3 polyunsaturated fatty acid (n-3 PUFA) supplementation has beneficial effects on the behavioral phenotype of the Fmr1 KO mice24,25.”

    From An iPSC-derived astrocyte model of fragile X syndrome exhibits dysregulated cholesterol homeostasis (2023) · cites this paper as [25]

    The Results (page unavailable) report that prenatal omega-3-enriched diet ameliorated reduced vocalization spectral purity in female Fmr1 knockout pups. The claim is broader than this finding: the same section says a control high-fat diet also ameliorated spectral purity, while omega-3 exposure decreased the number of calls in female knockouts. The male Results section reports no genotype-by-diet interaction for the vocalization measures tested. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Nolan, S. O., Hodges, S. L., Okoh, J. T., Binder, M. S., & Lugo, J. N.
Affiliations
Baylor University (US)
ORCID
Venue
Developmental Neuroscience
Publisher
S. Karger AG
Published
2020
Volume
42
Issue
2-4
Pages
94-104
ISSN
  • 0378-5866 (ISSN-L)
  • 1421-9859
DOI
10.1159/000509797
PMC
PMC7864857
OpenAlex
W3120373616
Cited by
10
References
2
Citations by year
2026: 2 · 2024: 1 · 2023: 4 · 2022: 2 · 2021: 1
FWCI
1.03
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Neurological Disorders and Stroke (R15NS088776)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Autism Spectrum Disorder Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Williams Syndrome Research
Keywords
omega-3 fatty acids, Fmr1 knockout mice, fragile X syndrome, ultrasonic vocalizations, social communication deficits, sex differences, fundamental frequency, spectral purity, autism spectrum disorder
MeSH terms
Autism Spectrum Disorder, Animals, Disease Models, Animal, Female, Male, Mutation, Pregnancy, Sex Characteristics, Vocalization, Animal, Fatty Acids, Omega-3, Mice, Knockout, Prenatal Nutritional Physiological Phenomena, Mice, Fragile X Mental Retardation Protein, Fragile X Messenger Ribonucleoprotein 1, Diet, High-Fat

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