The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
Expression of Multiple Isoforms of Nitric Oxide Synthase in Normal and Atherosclerotic Vessels
Abstract
Abstract Atherosclerosis is associated with reduced endothelium-derived relaxing factor bioactivity. To determine whether this is due to decreased synthesis of nitric oxide synthase (NOS), we examined normal and atherosclerotic human vessels by in situ hybridization and immunocytochemistry by using probes specific for endothelial (ecNOS), inducible (iNOS), and neuronal (nNOS) NOS isoforms. ecNOS was detected in endothelial cells overlying normal human aortas, fatty streaks, and advanced atherosclerotic lesions. A comparison of the relative expression of ecNOS to von Willebrand factor on serial sections of normal and atherosclerotic vessels indicated that there was a decrease in the number of endothelial cells expressing ecNOS in advanced lesions. iNOS and nNOS were not detected in normal vessels, but widespread production of these isoforms was found in early and advanced lesions associated with macrophages, endothelial cells, and mesenchymal-appearing intimal cells. These data suggest that there is (1) a loss of ecNOS expression by endothelial cells over advanced atherosclerotic lesions and (2) a significant increase in overall NOS synthesis by other cell types in advanced lesions composed of the ecNOS, nNOS, and iNOS isoforms. We hypothesize that the increased expression of NOS and presumably NO in atherosclerotic plaques may be related to cell death and necrosis in these tissues.
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Cites
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Cited by
Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress
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Self-citationRoles of vascular oxidative stress and nitric oxide in the pathogenesis of atherosclerosis
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Nitric oxide signalling in cardiovascular health and disease
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Gender, sex hormones, and vascular tone
Orshal, J. M., & Khalil, R. A. (2004) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00338.2003 · Cited by 650
Endothelium-derived nitric oxide and vascular physiology and pathology
Arnal, J.-F., Dinh-Xuan, A.-T., Pueyo, M., Darblade, B., & Rami, J. (1999) · Cellular and Molecular Life Sciences · doi:10.1007/s000180050358 · Cited by 313
Metadata
- Authors
- Wilcox, J. N., Subramanian, R. R., Sundell, C. L., Tracey, W. R., Pollock, J. S., Harrison, D. G., & Marsden, P. A.
- Venue
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Published
- 1997-11
- Volume
- 17
- Issue
- 11
- Pages
- 2479-2488
- ISSN
- 1079-5642 (ISSN-L)
- 1524-4636
- OpenAlex
- W2131750066
- Cited by
- 478
- References
- 56
- FWCI
- 11.10
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Expression of Multiple Isoforms of Nitric Oxide Synthase in Normal and Atherosclerotic Vessels. https://app.cochat.ai/papers/10.1161/01.atv.17.11.2479
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- Wilcox, J. N., Subramanian, R. R., Sundell, C. L., Tracey, W. R., Pollock, J. S., Harrison, D. G., & Marsden, P. A. (1997). Expression of Multiple Isoforms of Nitric Oxide Synthase in Normal and Atherosclerotic Vessels. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.atv.17.11.2479
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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