CoChat
Sign Up
Cited by 203Atherosclerosis and Cardiovascular DiseasesCerebrovascular and Carotid Artery DiseasesCoronary Interventions and Diagnostics

The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.

Induction of Atherosclerotic Plaque Rupture in Apolipoprotein E −/− Mice After Adenovirus-Mediated Transfer of p53

von der Thüsen, Jan H., van Vlijmen, Bart J.M., Hoeben, Rob C., Kockx, Mark M., Havekes, L.M., van Berkel, Theo J.C., Biessen, Erik A.L.·2002·Circulation
DOI 10.1161/01.cir.0000015502.97828.93
Open this paper in CoChat — free account

Abstract

Background — The presence of the tumor-suppressor gene p53 in advanced atherosclerotic plaques and the sensitivity to p53-induced cell death of smooth muscle cells isolated from these plaques have fueled speculation about the role of p53 in lesion destabilization and plaque rupture. In this study, we describe a strategy to promote (thrombotic) rupture of preexisting atherosclerotic lesions using p53-induced lesion remodeling. Methods and Results — Carotid atherogenesis was initiated in apolipoprotein E knockout mice by placement of a perivascular silastic collar. The resulting plaques were incubated transluminally with recombinant adenovirus carrying either a p53 or β-galactosidase ( lacZ ) transgene. p53 transfection was restricted to the smooth muscle cell-rich cap of the plaque and led to an increase in cap cell apoptosis 1 day after transfer. p53 overexpression resulted in a marked decrease in the cellular and extracellular content of the cap, reflected by a markedly reduced cap/intima ratio (0.21±0.04 versus 0.46±0.03, P <0.001). The latter is a characteristic feature of plaque vulnerability to rupture, and whereas spontaneous rupture of p53-treated lesions was rare, it was found in 40% of cases after treatment with the vasopressor compound phenylephrine ( P =0.003). Conclusions — We have demonstrated a potential role of p53-induced remodeling in atherosclerotic plaque destabilization. Being the first example of inducible rupture at a predefined location, this model offers a unique opportunity to delineate the processes that precede rupture and to evaluate plaque-stabilizing therapies.

Overview

Integrity signals

Registry

Crossref · 10.1161/01.cir.0000015502.97828.93

Cites

32 of 32 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 15

  1. 1996, Am J Pathol, 149, 367
  2. 1998, Eur Cytokine Netw, 9, 697
  3. 10.1161/atvb.18.10.1519 · DOI
  4. 1998, Circulation, 91, 2703
  5. 10.1161/circ.96.2.424 · DOI
  6. 10.1161/atvb.17.10.2218 · DOI
  7. 10.1161/atvb.17.10.2200 · DOI
  8. 1997, Cardiovasc Res, 45, 736
  9. 10.1161/res.81.4.591 · DOI
  10. 10.1161/res.88.8.780 · DOI
  11. 10.1161/circ.103.8.1164 · DOI
  12. 1994, Surgery, 116, 197
  13. 10.1161/res.74.3.8118960 · DOI
  14. 10.1161/res.77.6.1136 · DOI
  15. 1998, Cancer Res, 58, 4282

Cited by

Sorted by citations

See all 203

Related

See all

Metadata

Authors
von der Thüsen, J. H., van Vlijmen, B. J. M., Hoeben, R. C., Kockx, M. M., Havekes, L. M., van Berkel, T. J. C., & Biessen, E. A. L.
Affiliations
  • From the Division of Biopharmaceutics (J.H.v.d.T., T.J.C.v.B., E.A.L.B.), Leiden/Amsterdam Center for Drug Research; Molecular Virology Group, Department of Molecular Cell Biology (R.C.H.) and Department of Cardiology (J.H.v.d.T.), Leiden University Medical Center; and TNO-PG (B.J.M.v.V., L.M.H.), Leiden, the Netherlands; and Department of Pathology (M.M.K.), Middelheim Academic Hospital, Antwerp, Belgium.
  • Leiden University (NL)
ORCID
Venue
Circulation
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2002-04-30
Volume
105
Issue
17
Pages
2064-2070
ISSN
  • 0009-7322 (ISSN-L)
  • 1524-4539
DOI
10.1161/01.cir.0000015502.97828.93
OpenAlex
W2122574397
Cited by
203
References
32
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 6 · 2020: 3 · 2019: 6 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 7 · 2014: 8 · 2013: 10 · 2012: 8
FWCI
5.80
Type
journal article
Language
en
Open access
closed
Locations
  • Circulation (publishedVersion)
  • PubMed (publishedVersion)
  • TNO Repository (submittedVersion)
  • Repository hosted by TU Delft Library (TU Delft) (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Funders
Koninklijke Nederlandse Akademie van Wetenschappen
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cerebrovascular and Carotid Artery Diseases
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Coronary Interventions and Diagnostics
Keywords
atherosclerotic plaque rupture, p53, plaque destabilization, plaque remodeling, smooth muscle cell apoptosis, ApoE-deficient mice, adenoviral gene transfer, phenylephrine, plaque vulnerability
MeSH terms
Adenoviridae, Animals, Apolipoproteins E, Arteriosclerosis, beta-Galactosidase, Carotid Artery Diseases, Cell Division, Genetic Vectors, Immunohistochemistry, Muscle, Smooth, Vascular, Transfection, Biomarkers, Tumor Suppressor Protein p53, Apoptosis, Mice, Knockout, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review