The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Enhanced Inhibition of Neointimal Hyperplasia by Genetically Engineered Endothelial Progenitor Cells
Open accessAbstract
Background— Circulating endothelial progenitor cells (EPCs) have been reported previously. In this study, we examined the hypothesis that overexpression of vasculoprotective gene endothelial nitric oxide synthase (eNOS) and heme oxygenase-1 (HO-1) in EPCs enhances their ability to inhibit neointimal hyperplasia. Methods and Results— EPCs were isolated from rabbit peripheral blood, expanded in culture, and transduced with pseudotyped retroviral vectors expressing human eNOS (eNOS-EPCs), HO-1 (HO-1-EPCs), or green fluorescent protein (GFP-EPCs). Transduction efficiency of EPCs ex vivo was >90%. Four groups of rabbits (n=5 to 6 per group) were subjected to balloon angioplasty of the common carotid artery. Immediately after injury, ≈5×10 6 autologous eNOS-EPCs or HO-1-EPCs were transplanted into the injured vessel. Control animals received an equivalent number of GFP-EPCs or Ringer’s saline. Two weeks after transplantation, eNOS and HO-1 transgene transcripts and proteins were detected in the transduced rabbit vessels. Endothelialization was enhanced in the EPC-transplanted vessels independently of gene transfer. Neointimal thickening was significantly reduced in the GFP-EPC-treated vessels relative to the saline control. Neointima size was further reduced in vessels treated with eNOS-EPCs. Surprisingly, no additional reduction was seen in vessels treated with HO-1-EPCs relative to GFP-EPCs. Thrombosis occurred in ≈50% of the saline-treated vessels but was virtually absent in all EPC-transplanted vessels. Conclusions— We conclude that transplantation of autologous EPCs overexpressing eNOS in injured vessels enhances the vasculoprotective properties of the reconstituted endothelium, leading to inhibition of neointimal hyperplasia. This cell-based gene therapy strategy may be useful in treatment of vascular disease.
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Isolation of Putative Progenitor Endothelial Cells for Angiogenesis
Asahara, T., Murohara, T., Sullivan, A., Silver, M., van der Zee, R., Li, T., Witzenbichler, B., Schatteman, G., & Isner, J. M. (1997) · Science · doi:10.1126/science.275.5302.964 · Cited by 8,725
Asahara, T., Masuda, H., Takahashi, T., Kalka, C., Pastore, C., Silver, M., Kearne, M., Magner, M., & Isner, J. M. (1999) · Circulation Research · doi:10.1161/01.res.85.3.221 · Cited by 3,253
Takahashi, T., Kalka, C., Masuda, H., Chen, D., Silver, M., Kearney, M., Magner, M., Isner, J. M., & Asahara, T. (1999) · Nature Medicine · doi:10.1038/7434 · Cited by 2,423
Carbon monoxide has anti-inflammatory effects involving the mitogen-activated protein kinase pathway
Otterbein, L. E., Bach, F. H., Alam, J., Soares, M., Tao Lu, H., Wysk, M., Davis, R. J., Flavell, R. A., & Choi, A. M. K. (2000) · Nature Medicine · doi:10.1038/74680 · Cited by 2,153
De Caterina, R., Libby, P., Peng, H. B., Thannickal, V. J., Rajavashisth, T. B., Gimbrone, M. A., Shin, W. S., & Liao, J. K. (1995) · Journal of Clinical Investigation · doi:10.1172/jci118074 · Cited by 1,782
Evidence for Circulating Bone Marrow-Derived Endothelial Cells
Shi, Q., Rafii, S., Wu, M. H.-D., Wijelath, E. S., Yu, C., Ishida, A., Fujita, Y., Kothari, S., Mohle, R., Sauvage, L. R., et al. (1998) · Blood · doi:10.1182/blood.v92.2.362 · Cited by 1,695
Radomski, M. W., Palmer, R. M. J., & Moncada, S. (1987) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1987.tb11367.x · Cited by 1,164
Walter, D. H., Rittig, K., Bahlmann, F. H., Kirchmair, R., Silver, M., Murayama, T., Nishimura, H., Losordo, D. W., Asahara, T., & Isner, J. M. (2002) · Circulation · doi:10.1161/01.cir.0000018166.84319.55 · Cited by 918
Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo
Kaushal, S., Amiel, G. E., Guleserian, K. J., Shapira, O. M., Perry, T., Sutherland, F. W., Rabkin, E., Moran, A. M., Schoen, F. J., Atala, A., et al. (2001) · Nature Medicine · doi:10.1038/nm0901-1035 · Cited by 841
von der Leyen, H. E., Gibbons, G. H., Morishita, R., Lewis, N. P., Zhang, L., Nakajima, M., Kaneda, Y., Cooke, J. P., & Dzau, V. J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.4.1137 · Cited by 762
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Cited by
Paracrine Mechanisms in Adult Stem Cell Signaling and Therapy
Gnecchi, M., Zhang, Z., Ni, A., & Dzau, V. J. (2008) · Circulation Research · doi:10.1161/circresaha.108.176826 · Cited by 2,027
Self-citationPharmacological and Clinical Aspects of Heme Oxygenase
Abraham, N. G., & Kappas, A. (2008) · Pharmacological Reviews · doi:10.1124/pr.107.07104 · Cited by 1,115
Estrogen and the cardiovascular system
Knowlton, A. A., & Lee, A. R. (2012) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2012.03.007 · Cited by 489
Endothelial progenitor cells: characterization, pathophysiology, and possible clinical relevance
Hristov, M., & Weber, C. (2004) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2004.tb00474.x · Cited by 477
Vascular repair by endothelial progenitor cells
Zampetaki, A., Kirton, J. P., & Xu, Q. (2008) · Cardiovascular Research · doi:10.1093/cvr/cvn081 · Cited by 473
Metadata
- Authors
- Kong, D., Melo, L. G., Mangi, A. A., Zhang, L., Lopez-Ilasaca, M., Perrella, M. A., Liew, C. C., Pratt, R. E., & Dzau, V. J.
- Venue
- Circulation
- Published
- 2004-04-13
- Volume
- 109
- Issue
- 14
- Pages
- 1769-1775
- ISSN
- 0009-7322 (ISSN-L)
- 1524-4539
- OpenAlex
- W2065334138
- Cited by
- 288
- References
- 22
- FWCI
- 8.31
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kong, D., Melo, L. G., Mangi, A. A., Zhang, L., Lopez-Ilasaca, M., Perrella, M. A., Liew, C. C., Pratt, R. E., & Dzau, V. J. (2004). Enhanced Inhibition of Neointimal Hyperplasia by Genetically Engineered Endothelial Progenitor Cells. Circulation. https://doi.org/10.1161/01.cir.0000121732.85572.6f
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