The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
In Vivo klotho Gene Transfer Ameliorates Angiotensin II-Induced Renal Damage
Abstract
The klotho gene, originally identified by insertional mutagenesis in mice, suppresses the expression of multiple aging-associated phenotypes. This gene is predominantly expressed in the kidney. Recent studies have shown that expression of renal klotho gene is regulated in animal models of metabolic diseases and in humans with chronic renal failure. However, little is known about the mechanisms and the physiological relevance of the regulation of the expression of the klotho gene in the kidney in some diseased conditions. In the present study, we first investigated the role of angiotensin II in the regulation of renal klotho gene expression. Long-term infusion of angiotensin II downregulated renal klotho gene expression at both the mRNA and protein levels. This angiotensin II-induced renal klotho downregulation was an angiotensin type 1 receptor-dependent but pressor-independent event. Adenovirus harboring mouse klotho gene (ad-klotho, 3.3×10 10 plaque forming units) was also intravenously administered immediately before starting angiotensin II infusion in some rats. This resulted in a robust induction of Klotho protein in the liver at day 4, which was still detectable 14 days after the gene transfer. Ad-klotho gene transfer, but not ad-lacZ gene transfer, caused an improvement of creatinine clearance, decrease in urinary protein excretion, and amelioration of histologically demonstrated tubulointerstitial damage induced by angiotensin II administration. Our data suggest that downregulation of the renal klotho gene may have an aggravative role in the development of renal damage induced by angiotensin II, and that induction of the klotho gene may have therapeutic possibilities in treating angiotensin II-induced end organ damage.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
Self-citationSeverely Reduced Production of Klotho in Human Chronic Renal Failure Kidney
Koh, N., Fujimori, T., Nishiguchi, S., Tamori, A., Shiomi, S., Nakatani, T., Sugimura, K., Kishimoto, T., Kinoshita, S., Kuroki, T., et al. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.4226 · Cited by 494
Klotho Protein Protects against Endothelial Dysfunction
Saito, Y., Yamagishi, T., Nakamura, T., Ohyama, Y., Aizawa, H., Suga, T., Matsumura, Y., Masuda, H., Kurabayashi, M., Kuro-o, M., et al. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.8943 · Cited by 274
Self-citationKlotho protein protects against endothelial dysfunction
齋藤, 勇. (1999) · Medical Entomology and Zoology · doi:10.11501/3152652 · Cited by 222
Gavras, H., Brunner, H. R., Laragh, J. H., Vaughan, E. D., Koss, M., Cote, L. J., & Gavras, I. (1975) · Circulation Research · doi:10.1161/01.res.36.2.300 · Cited by 202
Establishment of the Anti-Klotho Monoclonal Antibodies and Detection of Klotho Protein in Kidneys
Kato, Y., Arakawa, E., Kinoshita, S., Shirai, A., Furuya, A., Yamano, K., Nakamura, K., Iida, A., Anazawa, H., Koh, N., et al. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.2009 · Cited by 159
Ohyama, Y., Kurabayashi, M., Masuda, H., Nakamura, T., Aihara, Y., Kaname, T., Suga, T., Arai, M., Aizawa, H., Matsumura, Y., et al. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9576 · Cited by 154
Aizawa, T., Ishizaka, N., Taguchi, J., Nagai, R., Mori, I., Tang, S., Ingelfinger, J. R., & Ohno, M. (2000) · Hypertension · doi:10.1161/01.hyp.35.3.800 · Cited by 146
Self-citationAngiotensin II–Induced Hypertension Increases Heme Oxygenase-1 Expression in Rat Aorta
Ishizaka, N., León, H. D., Laursen, J. B., Fukui, T., Wilcox, J. N., Keulenaer, G. W. D., Griendling, K. K., & Alexander, R. W. (1997) · Circulation · doi:10.1161/01.cir.96.6.1923 · Cited by 129
Self-citationDownregulation of theKlothoGene in the Kidney under Sustained Circulatory Stress in Rats
Aizawa, H., Saito, Y., Nakamura, T., Inoue, M., Imanari, T., Ohyama, Y., Matsumura, Y., Masuda, H., Oba, S., Mise, N., et al. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9246 · Cited by 113
Self-citation
Cited by
Regulation and Function of the FGF23/Klotho Endocrine Pathways
Martin, A., David, V., & Quarles, L. D. (2012) · Physiological Reviews · doi:10.1152/physrev.00002.2011 · Cited by 594
Hu, M. C., Shiizaki, K., Kuro-o, M., & Moe, O. W. (2013) · Annual Review of Physiology · doi:10.1146/annurev-physiol-030212-183727 · Cited by 587
Doi, S., Zou, Y., Togao, O., Pastor, J. V., John, G. B., Wang, L., Shiizaki, K., Gotschall, R., Schiavi, S., Yorioka, N., et al. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.174037 · Cited by 532
de Borst, M. H., Vervloet, M. G., ter Wee, P. M., & Navis, G. (2011) · Journal of the American Society of Nephrology · doi:10.1681/asn.2010121251 · Cited by 282
Song, X., Di Giovanni, V., He, N., Wang, K., Ingram, A., Rosenblum, N. D., & Pei, Y. (2009) · Human Molecular Genetics · doi:10.1093/hmg/ddp165 · Cited by 256
Metadata
- Authors
- Mitani, H., Ishizaka, N., Aizawa, T., Ohno, M., Usui, S.-I., Suzuki, T., Amaki, T., Mori, I., Nakamura, Y., Sato, M., Nangaku, M., Hirata, Y., & Nagai, R.
- Venue
- Hypertension
- Published
- 2002-04
- Volume
- 39
- Issue
- 4
- Pages
- 838-843
- ISSN
- 0194-911X (ISSN-L)
- 1524-4563
- OpenAlex
- W2123069635
- Cited by
- 256
- References
- 13
- FWCI
- 3.32
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: In Vivo klotho Gene Transfer Ameliorates Angiotensin II-Induced Renal Damage. https://app.cochat.ai/papers/10.1161/01.hyp.0000013734.33441.ea
- The paper
- Mitani, H., Ishizaka, N., Aizawa, T., Ohno, M., Usui, S.-I., Suzuki, T., Amaki, T., Mori, I., Nakamura, Y., Sato, M., Nangaku, M., Hirata, Y., & Nagai, R. (2002). In Vivo klotho Gene Transfer Ameliorates Angiotensin II-Induced Renal Damage. Hypertension. https://doi.org/10.1161/01.hyp.0000013734.33441.ea
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
