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Preactivated Peripheral Blood Monocytes in Patients With Essential Hypertension
Open accessAbstract
Abstract —The purpose of this study was to investigate the possible involvement of human peripheral blood monocytes in the pathology of hypertensive disease. We determined the in vitro secretion patterns of proinflammatory cytokines obtained from isolated peripheral monocytes from normal controls and from hypertensive patients either after in vitro stimulation with angiotensin II (Ang II) with or without preincubation with an Ang II type 1 receptor antagonist (losartan) or after stimulation with lipopolysaccharide. Blood samples were obtained from 22 patients with essential hypertension (before any drug administration or after interruption of antihypertensive therapy) and from 24 normotensive healthy individuals used as a control group. Peripheral blood monocytes were isolated by density gradient centrifugation and plastic adherence. The state of monocyte activity was determined by the capacity to secrete tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6, (IL-6) either spontaneously or after stimulation. Cytokine concentrations were determined in culture supernatants by specific ELISA. Proinflammatory cytokine levels were assessed by semiquantitative reverse transcribed polymerase chain reaction. After stimulation with Ang II, the IL-1β secretion of peripheral blood monocytes was significantly increased in hypertensive patients versus healthy individuals ( P <0.05). In contrast, in monocytes preincubated with losartan before exposure to Ang II, IL-1β secretion was diminished in both groups to comparable levels. The secretion of IL-1β and TNF-α was significantly increased in peripheral blood monocytes from hypertensive patients versus healthy individuals after stimulation with lipopolysaccharide (TNF-α, P <0.02; IL-1β, P <0.05). Upregulation of IL-1β and TNF-α secretion in peripheral blood monocytes from hypertensive patients was also seen at the RNA level. Our results indicate preactivated peripheral blood monocytes in hypertensive patients. Ang II may be directly involved in the process of monocyte activation.
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“water retention hormones such as angiotensin II have proinflammatory activities [37].”
From Evolutionary medicine and chronic inflammatory state—known and new concepts in pathophysiology (2012) · cites this paper as [37]
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Metadata
- Authors
- Dörffel, Y., Lätsch, C., Stuhlmüller, B., Schreiber, S., Scholze, S., Burmester, G. R., & Scholze, J.
- Affiliations
- From the Outpatient Clinics of Internal Medicine (Y.D., C.L., J.S.), and the Department of Rheumatology and Clinical Immunology (B.S., S. Scholze, G.R.B.), Charité University Hospital, Humboldt University, Berlin, and First Medical Department, Christian Albrecht University, Kiel, (S. Schreiber) Germany.
- Christian-Albrechts-Universität zu Kiel (DE)
- Venue
- Hypertension
- Published
- 1999-07
- Volume
- 34
- Issue
- 1
- Pages
- 113-117
- ISSN
- 0194-911X (ISSN-L)
- 1524-4563
- OpenAlex
- W2169025417
- Cited by
- 262
- References
- 32
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 7 · 2023: 11 · 2022: 9 · 2021: 12 · 2020: 9 · 2019: 9 · 2018: 9 · 2017: 15 · 2016: 9 · 2015: 9 · 2014: 7 · 2013: 10 · 2012: 11
- FWCI
- 4.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Hypertension (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Blood Pressure and Hypertension Studies
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Keywords
- essential hypertension, peripheral blood monocytes, monocyte activation, inflammatory cytokines, angiotensin II, angiotensin II type 1 receptor, losartan, IL-1β, TNF-α, interleukin-6, lipopolysaccharide
- MeSH terms
- Adult, Aged, Angiotensin II, Cells, Cultured, Female, Humans, Hypertension, Interleukin-1, Lipopolysaccharides, Male, Middle Aged, Monocytes, Reference Values, Tumor Necrosis Factor-alpha, Interleukin-6, Cytokines, Reverse Transcriptase Polymerase Chain Reaction
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