The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Protein Kinase G Transmits the Cardioprotective Signal From Cytosol to Mitochondria
Abstract
Ischemic and pharmacological preconditioning can be triggered by an intracellular signaling pathway in which G i -coupled surface receptors activate a cascade including phosphatidylinositol 3-kinase, endothelial nitric oxide synthase, guanylyl cyclase, and protein kinase G (PKG). Activated PKG opens mitochondrial K ATP channels (mitoK ATP ) which increase production of reactive oxygen species. Steps between PKG and mitoK ATP opening are unknown. We describe effects of adding purified PKG and cGMP on K + transport in isolated mitochondria. Light scattering and respiration measurements indicate PKG induces opening of mitoK ATP similar to K ATP channel openers like diazoxide and cromakalim in heart, liver, and brain mitochondria. This effect was blocked by mitoK ATP inhibitors 5-hydroxydecanoate, tetraphenylphosphonium, and glibenclamide, PKG-selective inhibitor KT5823, and protein kinase C (PKC) inhibitors chelerythrine, Ro318220, and PKC-ε peptide antagonist εV 1-2 . MitoK ATP are opened by the PKC activator 12-phorbol 13-myristate acetate. We conclude PKG is the terminal cytosolic component of the trigger pathway; it transmits the cardioprotective signal from cytosol to inner mitochondrial membrane by a pathway that includes PKC-ε.
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“MitoSNO has been reported to be protective in mice with cardiomyocyte deficiency of protein kinase G18, indicating that the cardio-protection conferred by MitoSNO is not due to nonmitochondrial pathways that operate through cyclic GMP–protein kinase G signaling3,6”
From Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I (2013) · cites this paper as [6]
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Metadata
- Authors
- Costa, A. D. T., Garlid, K. D., West, I. C., Lincoln, T. M., Downey, J. M., Cohen, M. V., & Critz, S. D.
- Affiliations
- Portland State University (US)
- Venue
- Circulation Research
- Published
- 2005-08-19
- Volume
- 97
- Issue
- 4
- Pages
- 329-336
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2151589110
- Cited by
- 307
- References
- 44
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 1 · 2023: 5 · 2022: 4 · 2021: 6 · 2020: 8 · 2019: 8 · 2018: 8 · 2017: 7 · 2016: 7 · 2015: 15 · 2014: 15 · 2013: 20 · 2012: 14
- FWCI
- 22.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Circulation Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL20648, HL35673, HL-67842, R01HL050688, HL50688, R01HL020648, R01HL067842)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Ischemia and Reperfusion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
- Keywords
- protein kinase G, mitochondrial KATP channels, cardioprotection, preconditioning, cyclic GMP, protein kinase C epsilon, ischemia, reactive oxygen species, mitochondrial respiration, potassium transport, endothelial nitric oxide synthase, guanylate cyclase, phosphatidylinositol 3-kinase, signal transduction
- MeSH terms
- Adenosine Triphosphate, Animals, Brain, Cytosol, Cyclic GMP, Male, Mitochondria, Heart, Mitochondria, Liver, Oxygen Consumption, Protein Kinase C, Tetradecanoylphorbol Acetate, Tetraethylammonium Compounds, Potassium Channels, Signal Transduction, Rats, Wistar, Cyclic GMP-Dependent Protein Kinases, Ischemic Preconditioning, Myocardial, Rats
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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