The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
AMP-Activated Protein Kinase Promotes the Differentiation of Endothelial Progenitor Cells
Open accessAbstract
OBJECTIVE: Endothelial progenitor cells (EPCs) can differentiate into endothelial cells (ECs) and participate in postnatal vasculogenesis, but the mechanism of EPC differentiation remains largely unknown. We investigated the role of AMP-activated protein kinase (AMPK) in EPC differentiation and functions. METHODS AND RESULTS: Vascular endothelial growth factor caused the phosphorylation of AMPK, acetyl-coenzymeA (CoA) carboxylase (ACC), and eNOS in human cord blood-derived EPCs. The expression of EC markers, including VE-cadherin and intercellular adhesion molecule1 (ICAM-1), was also increased but blocked by Compound C, an AMPK inhibitor. AICAR, an AMPK agonist, increased the phosphorylation of ACC and eNOS and the expression of EC markers in a time- and dose-dependent manner, which reinforces the positive effect of AMPK on EPC differentiation. The effects of AICAR could be blocked by treatment with L-NAME, an eNOS inhibitor. Functionally, AICAR increased but Compound C decreased the angiogenesis of EPCs in vitro and in vivo. Furthermore, lovastatin promoted the activation of AMPK and eNOS, the expression of EC markers, tube formation, adhesion, and in vivo vasculogenesis of EPCs, which could be blocked by treatment with Compound C. CONCLUSIONS: The activation of eNOS by AMPK during EPC differentiation provides a novel mechanism for the pleiotropic effects of statins in benefiting the cardiovascular system.
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“As discussed previously, a similar general outcome of metformin, AICAR, atheroprotective flow, and statins on ECs can be found through AMPK activation [245,250,251].”
From AMPK, Mitochondrial Function, and Cardiovascular Disease (2020) · cites this paper as [251]
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Cites
Asahara, T., Masuda, H., Takahashi, T., Kalka, C., Pastore, C., Silver, M., Kearne, M., Magner, M., & Isner, J. M. (1999) · Circulation Research · doi:10.1161/01.res.85.3.221 · Cited by 3,263
Urbich, C., & Dimmeler, S. (2004) · Circulation Research · doi:10.1161/01.res.0000137877.89448.78 · Cited by 2,388
Peichev, M., Naiyer, A. J., Pereira, D., Zhu, Z., Lane, W. J., Williams, M., Oz, M., Hicklin, D. J., Witte, L., Moore, M. A. S., et al. (2000) · Blood · doi:10.1182/blood.v95.3.952.003k27_952_958 · Cited by 2,268
Asahara, T., Takahashi, T., Masuda, H., Kalka, C., Chen, D., Iwaguro, H., Inai, Y., Silver, M., & Isner, J. M. (1999) · The EMBO Journal · doi:10.1093/emboj/18.14.3964 · Cited by 1,851
AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor Cells
Yin, A. H., Miraglia, S. J., Zanjani, E. D., Almeida-Porada, G. D., Ogawa, M., Leary, A. G., Olweus, J., Kearney, J. F., & Buck, D. W. (1997) · Blood · doi:10.1182/blood.v90.12.5002 · Cited by 1,821
Dimmeler, S., Aicher, A., Vasa‐Nicotera, M., Mildner-Rihm, C., Adler, K., Tiemann, M., Rütten, H., Fichtlscherer, S., Martin, H., & Zeiher, A. M. (2001) · Journal of Clinical Investigation · doi:10.1172/jci13152 · Cited by 1,206
Hur, J., Yoon, C., Kim, H.-S., Choi, J., Kang, H., Hwang, K.-K., Oh, B., Lee, M. M., & Park, Y.-B. (2003) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000114236.77009.06 · Cited by 1,205
AMP-activated protein kinase signaling in metabolic regulation
Long, Y. C. (2006) · Journal of Clinical Investigation · doi:10.1172/jci29044 · Cited by 1,125
Dimmeler, S., Aicher, A., Vasa‐Nicotera, M., et al. (2001) · Journal of Clinical Investigation · doi:10.1172/jci200113152 · Cited by 1,025
In vitro differentiation of endothelial cells from AC133-positive progenitor cells
Gehling, U. M., Ergün, S., Schumacher, U., Wagener, C., Pantel, K., Otte, M., Schuch, G., Schafhausen, P., Mende, T., Kilic, N., et al. (2000) · Blood · doi:10.1182/blood.v95.10.3106 · Cited by 1,018
Vasa‐Nicotera, M., Fichtlscherer, S., Adler, K., Aicher, A., Martin, H., Zeiher, A. M., & Dimmeler, S. (2001) · Circulation · doi:10.1161/hc2401.092816 · Cited by 1,010
Adiponectin Stimulates Production of Nitric Oxide in Vascular Endothelial Cells
Chen, H., Montagnani, M., Funahashi, T., Shimomura, I., & Quon, M. J. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m307878200 · Cited by 995
Statin Therapy Accelerates Reendothelialization
Walter, D. H., Rittig, K., Bahlmann, F. H., Kirchmair, R., Silver, M., Murayama, T., Nishimura, H., Losordo, D. W., Asahara, T., & Isner, J. M. (2002) · Circulation · doi:10.1161/01.cir.0000018166.84319.55 · Cited by 918
Bolster, D. R., Crozier, S. J., Kimball, S. R., & Jefferson, L. S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200171200 · Cited by 851
Ouchi, N., Kobayashi, H., Kihara, S., Kumada, M., Sato, K., Inoue, T., Funahashi, T., & Walsh, K. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310389200 · Cited by 759
HMG-CoA reductase inhibitor mobilizes bone marrow–derived endothelial progenitor cells
Llevadot, J., Murasawa, S., Kureishi, Y., Uchida, S., Masuda, H., Kawamoto, A., Walsh, K., Isner, J. M., & Asahara, T. (2001) · Journal of Clinical Investigation · doi:10.1172/jci13131 · Cited by 701
HMG-CoA reductase inhibitor mobilizes bone marrow–derived endothelial progenitor cells
Llevadot, J., Murasawa, S., Kureishi, Y., et al. (2001) · Journal of Clinical Investigation · doi:10.1172/jci200113131 · Cited by 611
AMPK: A Key Sensor of Fuel and Energy Status in Skeletal Muscle
Hardie, D. G., & Sakamoto, K. (2006) · Physiology · doi:10.1152/physiol.00044.2005 · Cited by 536
Endothelial progenitor cells for postnatal vasculogenesis
Asahara, T., & Kawamoto, A. (2004) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00330.2003 · Cited by 530
Horman, S., Browne, G. J., Krause, U., Patel, J. V., Vertommen, D., Bertrand, L., Lavoinne, A., Hue, L., Proud, C. G., & Rider, M. H. (2002) · Current Biology · doi:10.1016/s0960-9822(02)01077-1 · Cited by 467
Davis, B. J., Xie, Z., Viollet, B., & Zou, M. (2006) · Diabetes · doi:10.2337/diabetes.55.02.06.db05-1064 · Cited by 465
Landmesser, U., Engberding, N., Bahlmann, F. H., Schaefer, A., Wiencke, A., Heineke, A., Spiekermann, S., Hilfiker‐Kleiner, D., Templin, C., Kotlarz, D., et al. (2004) · Circulation · doi:10.1161/01.cir.0000143232.67642.7a · Cited by 423
Woods, A., Azzout‐Marniche, D., Foretz, M., STEIN, S. C., Lemarchand, P., Ferré, P., Foufelle, F., & Carling, D. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.18.6704-6711.2000 · Cited by 417
Morrow, V. A., Foufelle, F., Connell, J., Petrie, J. R., Gould, G. W., & Salt, I. P. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212831200 · Cited by 361
Shibata, R., Ouchi, N., Kihara, S., Sato, K., Funahashi, T., & Walsh, K. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m402558200 · Cited by 336
Iglesias, M. A., Ye, J.-M., Frangioudakis, G., Saha, A. K., Tomas, E., Ruderman, N. B., Cooney, G. J., & Kraegen, E. W. (2002) · Diabetes · doi:10.2337/diabetes.51.10.2886 · Cited by 290
Statins Activate AMP-Activated Protein Kinase In Vitro and In Vivo
Sun, W., Lee, T., Zhu, M., Gu, C., Wang, Y., Zhu, Y., & Shyy, J. Y.-J. (2006) · Circulation · doi:10.1161/circulationaha.106.630194 · Cited by 264
Self-citationA homing mechanism for bone marrow-derived progenitor cell recruitment to the neovasculature
Jin, H. (2006) · Journal of Clinical Investigation · doi:10.1172/jci24751 · Cited by 224
Zhang, Y., Lee, T.-S., Kolb, E. M., Sun, K., Lu, X., Sladek, F. M., Kassab, G. S., Garland, T., & Shyy, J. Y.-J. (2006) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000221230.08596.98 · Cited by 212
Self-citationNitric oxide modulation of human leukemia cell differentiation and gene expression
Magrinat, G., Mason, S. N., Shami, P., & Weinberg, J. (1992) · Blood · doi:10.1182/blood.v80.8.1880.1880 · Cited by 170
Drew, B. G., Fidge, N., Beaumier-Gallon, G., Kemp, B. E., & Kingwell, B. A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0306266101 · Cited by 169
Statin Therapy in Acute Coronary Syndromes
Sposito, A. C., & Chapman, M. J. (2002) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000032033.39301.6a · Cited by 157
Urbich, C., & Dimmeler, S. (2005) · Kidney International · doi:10.1111/j.1523-1755.2005.00261.x · Cited by 146
Kalinowski, L., Dobrucki, L. W., Brovkovych, V. M., & Maliñski, T. (2002) · Circulation · doi:10.1161/hc0802.104283 · Cited by 134
Ni, W., Egashira, K., Kataoka, C., Kitamoto, S., Koyanagi, M., Inoue, S., & Takeshita, A. (2001) · Circulation Research · doi:10.1161/hh1701.096614 · Cited by 129
Maycock, C. A. A., Muhlestein, J. B., Horne, B. D., Carlquist, J. F., Bair, T. L., Pearson, R. R., Li, Q., & Anderson, J. L. (2002) · Journal of the American College of Cardiology · doi:10.1016/s0735-1097(02)02477-4 · Cited by 127
Schulz, E., Anter, E., Zou, M., & Keaney, J. F. (2005) · Circulation · doi:10.1161/circulationaha.105.546812 · Cited by 114
Nitric oxide promotes differentiation of rat white preadipocytes in culture
Yan, H., Aziz, E., Shillabeer, G., Wong, A., Shanghavi, D. S., Kermouni, A., Abdel-Hafez, M., & Lau, D. C. W. (2002) · Journal of Lipid Research · doi:10.1194/jlr.m200305-jlr200 · Cited by 89
Liu, C., Wang, S., Deb, A., Nath, K. A., Katušić, Z. S., McConnell, J. P., & Caplice, N. M. (2005) · Circulation Research · doi:10.1161/01.res.0000174612.90094.fd · Cited by 82
Fißlthaler, B., Fleming, I., Keserü, B., Walsh, K., & Busse, R. (2007) · Circulation Research · doi:10.1161/01.res.0000257747.74358.1c · Cited by 75
Arginine Vasopressin-mediated Cardiac Differentiation
Gassanov, N., Jankowski, M., Danalache, B. A., Wang, D., Grygorczyk, R., Hoppe, U. C., & Gutkowska, J. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m610769200 · Cited by 44
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Donato, A. J., Morgan, R. G., Walker, A. E., & Lesniewski, L. A. (2015) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2015.01.021 · Cited by 538
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Metadata
- Authors
- Li, X., Han, Y., Pang, W., Li, C., Xie, X., Shyy, J. Y.-J., & Zhu, Y.
- Affiliations
- University of California, Riverside (US)
- Venue
- Arteriosclerosis Thrombosis and Vascular Biology
- Published
- 2008-10
- Volume
- 28
- Issue
- 10
- Pages
- 1789-1795
- ISSN
- 1079-5642 (ISSN-L)
- 1524-4636
- PMC
- PMC2737684
- OpenAlex
- W2121972161
- Cited by
- 113
- References
- 39
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 8 · 2019: 3 · 2018: 9 · 2017: 5 · 2016: 3 · 2015: 6 · 2014: 9 · 2013: 8 · 2012: 20
- FWCI
- 4.39
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Arteriosclerosis Thrombosis and Vascular Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL-77448, R01HL089940, R01HL077448)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- AMPK, endothelial progenitor cells, endothelial differentiation, endothelial nitric oxide synthase, acetyl-CoA carboxylase, AICAR, compound C, lovastatin, statins, angiogenesis, vasculogenesis, vascular endothelial growth factor, VE-cadherin, ICAM-1, L-NAME, tube formation
- MeSH terms
- Acetyl-CoA Carboxylase, Aminoimidazole Carboxamide, Animals, Cell Differentiation, Cells, Cultured, Enzyme Inhibitors, Fetal Blood, Humans, Lovastatin, Mice, Nude, Multienzyme Complexes, Nitric Oxide, Phosphorylation, Pyrazoles, Pyrimidines, Ribonucleotides, Signal Transduction, Biomarkers, Protein Serine-Threonine Kinases, Neovascularization, Physiologic, Hydroxymethylglutaryl-CoA Reductase Inhibitors, NG-Nitroarginine Methyl Ester, Enzyme Activators, Cord Blood Stem Cell Transplantation, Vascular Endothelial Growth Factor A, Endothelial Cells, Mice, Nitric Oxide Synthase Type III, Fetal Stem Cells, AMP-Activated Protein Kinases
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